数字农科院2.0

Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV)

文献类型： 外文期刊

作者： Zhigang Cao;Li Yi;Xiangnan Liu;Jinyuan Shang;Yuening Cheng;Erkai Feng;Xingyu Liu;Yuping Fan;Xiaoliang Hu;Wenlong Cai;Feng Cong;Shipeng Cheng

作者机构：

关键词： CDV;detection;FLFA;fluorescent;microspheres

期刊名称： Frontiers in Veterinary Science

ISSN： 2297-1769

年卷期： 2024 年 11 卷

页码：

收录情况： SCIE（2024版）

摘要： Canine distemper virus (CDV) is a highly contagious and potentially lethal virus that affects dogs and other members of the Canidae family, including wolves, foxes, and coyotes. Here, we present a fluorescent lateral flow immunoassay (FLFA) platform for the detection of CDV, which utilizes fluorescent microspheres - fusion protein monoclonal antibody (mAb)-labeled monoclonal antibody. The assay detected CDV within 5 min, with a detection limit threshold of 3 × 102 TCID50/mL. Notably, the assay demonstrated no cross-reactivity with canine parvovirus, canine coronavirus, canine adenovirus, feline calicivirus, feline herpesvirus, or feline parvovirus. Field and clinical applicability of the assay was evaluated using 63 field samples, including 30 canine fecal samples, 18 swab samples, and 15 blood samples. The coincidence rate between the detection results of clinical samples obtained through FLFA and reverse transcription polymerase chain reaction (RT-PCR) was 96.83%. Thus, this assay offers a significant advancement for the rapid diagnosis of CDV at the point of care.

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