数字农科院2.0

Evolution of mitogen-activated protein kinase in plants and AtMAPK6's role in heat stress response in Arabidopsis

文献类型： 外文期刊

作者： Min Jiang;Pedro García-Caparrós;Zhengwei Wang;Yuxin Zhang;Yiying Liao;Yiqing Gong;Changling Zhao;Guosong Wen

作者机构：

关键词： Evolution;Heat stress;Hypocotyl elongation;MAPK;PIF4;Protein-protein interaction

期刊名称： Plant Science

ISSN： 1873-2259

年卷期： 2025 年 362 卷

页码：

收录情况： SCIE（2025版）

摘要： Mitogen-activated protein kinases (MAPKs) are essential components of tightly regulated signaling pathways that play critical roles in various aspects of plant biology, including growth, development, and defense responses. Despite their significance, the evolutionary origins and processes that have shaped their functional diversity across different plant species still require further investigation. This study elucidated the evolutionary history of MAPK homologs, tracing their lineage to green algae and conducting a comprehensive analysis of their evolutionary trajectory. Our results revealed that MAPK6 orthologs emerged as a spermatophyte-specific innovation, marked by specific patterns of motif gain and/or loss, as well as an increased purifying selection pressure. These features reflect their lineage specific adaptations and functional specialization. Synteny analysis revealed that whole-genome duplication (WGD) events occurred independently in the ancestors of monocotyledons and dicotyledons, contributing substantially to the diversification and formation of the major extant plant lineages. Codon usage analysis demonstrated that different species exhibit varying species-specific preferences, with algae and mosses showing a distinct bias toward G3s, C3s, GC3s, and overall GC content, suggesting potential adaptations or optimizations for translational efficiency. Furthermore, the results of weighted gene co-expression network analysis (WGCNA) reported that the lightcyan module was significantly negatively associated with heat stress response. Within this module, Arabidopsis thaliana MAPK6 (AthMAPK6, commonly known as AtMAPK6) was identified as a hub gene in the co-expression network. Functional validation using mutant lines demonstrated that AtMAPK6 was involved in thermomorphogenesis under high-temperature conditions. Notably, the N-terminal region of AtPIF4 was essential for this interaction between these two proteins. This study collectively provides significant insights into the functional role of AtMAPK6 in modulating plant responses to heat stress and offers a broader understanding of the evolutionary perspective on the diversification and specialization of MAPK homologs across plant lineages.

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