数字农科院2.0

Transactivation of Dek601 by the Opaque2-OHP2-EREB58-EREB177 module regulates kernel development in maize

文献类型： 外文期刊

作者： Wei, Qianhan;Fan, Kaijian;Zhao, Anqi;Fu, Qinghui;Miao, Rong;Zhang, Yawen;Dou, Xupeng;Su, Yuhao;Ren, Zhenjing;Peng, Yunling;Wang, Jianhua;Liu, Yunjun

作者机构：

关键词： maize; DEK601; kernel development

期刊名称： PLANT PHYSIOLOGY

ISSN： 0032-0889

年卷期： 2025 年 199 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： BAHD acyltransferases are a specialized class of plant enzymes involved in the acylation of secondary metabolites, yet their roles in seed development remain poorly understood. In this study, we identified the defective kernel 601 (dek601) mutant in the maize (Zea mays) W22 genetic background, which exhibits defective embryo and endosperm development, leading to smaller and shrunken kernels compared to the wild type. Using positional cloning, we localized the causal gene, Zm00001d039535 (also known as Disorganized wall 1, Dow1), to chromosome 3. A mutation in this gene causes a frameshift and disrupts protein function, which was confirmed through complementation tests. Dek601 encodes a BAHD family acyltransferase, and its expression is regulated by the transcription factors Opaque2 (O2), Ethylene-Responsive Element Binding Protein 58 (EREB58), and Ethylene-Responsive Element Binding Protein 177 (EREB177). These factors bind to the Dek601 promoter and modulate its expression. Furthermore, DEK601 interacts with the acyl-activating enzyme 19 (ZmAAE19), suggesting their potential functional synergy in downstream enzymatic processes. Metabolomic analysis of dek601 kernels revealed significant shifts in metabolic profiles, including a downregulation of carbohydrate compounds and an upregulation of amino acids and metabolites from the phenylpropanoid and flavonoid pathways. This study demonstrates that the Opaque2-OHP2-EREB58-EREB177 regulatory module controls Dek601 expression, and DEK601 and ZmAAE19 likely collaborate to influence metabolite biosynthesis, thereby regulating kernel development in maize. The transcription factor module directly activates the Defective Kernel 601 acyltransferase, which influences metabolite changes during maize kernel development.

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