数字农科院2.0

Integration of Green and Far-Red Light with Red-Blue Light Enhances Shoot Multiplication in Micropropagated Strawberry

文献类型： 外文期刊

作者： Yali Li;Ping Huang;Xia Qiu;Feiyu Zhu;Hongwen Chen;Si Wang;Jiaxian He;Yadan Pang;Hui Ma;Fang Wang

作者机构：

关键词： circadian rhythm;light spectrum;phytochrome signaling;tissue culture;transcriptome analysis

期刊名称： Horticulturae

ISSN： 2311-7524

年卷期： 2025 年 11 卷 6 期

页码：

收录情况： SCIE（2025版）

摘要： Light spectral composition critically regulates plant morphogenesis and molecular adaptation in controlled environments. This study investigated the synergistic effects of three light spectra, red-blue (RB, 7:3), red-blue-green (RGB, 7:3:1), and red-blue-far-red (RBFR, 7:3:1), on multiplication, morphogenesis, physiological traits, and transcriptomic dynamics in tissue-cultured strawberry (Fragaria × ananassa cv. ‘Benihoppe’). After 28 days of cultivation under controlled conditions (25 °C/22 °C day/night, 50 μmol·m−2·s−1 PPFD), RBFR and RGB treatments significantly enhanced shoot multiplication (38.8% and 24.2%, respectively), plant height, and callus biomass compared to RB light. RGB elevated chlorophyll a and b by 1.8- and 1.6-fold, respectively, while RBFR increased soluble protein content by 16%. Transcriptome analysis identified 144 and 376 differentially expressed genes (DEGs) under RGB and RBFR, respectively, enriched in pathways linked to circadian rhythm, auxin transport, and photosynthesis. Far-red light upregulated light signaling and photomorphogenesis genes, whereas green light enhanced chlorophyll biosynthesis while suppressing stress-responsive genes. These findings elucidate the spectral-specific regulatory mechanisms underlying strawberry micropropagation and provide a framework for optimizing multispectral LED systems in controlled-environment horticulture.

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