数字农科院2.0

Genome-Wide Analysis of Callose Synthase (CALS) Genes in Cabbage (Brassica oleracea var. capitata L.): Identification and Expression Profiling During Hyaloperonospora parasitica Infection

文献类型： 外文期刊

作者： Li, Jiamin;Wu, Yuankang;Yao, Xuehui;Yang, Limei;Zhuang, Mu;Lv, Honghao;Wang, Yong;Ji, Jialei;Zhang, Yangyong

作者机构：

关键词： Brassica oleracea;callose synthase enzyme;downy mildew;gene family;expression analysis

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1661-6596

年卷期： 2025 年 26 卷 21 期

页码：

收录情况： SCIE（2025版）

摘要： Callose synthase (CALS) genes are known to play critical roles in microspore development and in plant responses to diverse biotic and abiotic stresses. While the role of CALS genes has been extensively characterized in several plant species, their homologs in Brassica oleracea (BoCALS) remain understudied. In this study, 15 BoCALS genes were identified in B. oleracea genome, distributed across eight chromosomes. All BoCALS proteins contain Glucan-synthase and Fks1 domains. Phylogenetic analysis grouped BoCALS and their homologs from Arabidopsis thaliana and Brassica rapa into three distinct Clusters (I-III), revealing conserved evolutionary relationships within the Brassicaceae family. Collinearity analysis showed that AtCALS genes of Arabidopsis have multiple orthologs in B. oleracea. Analysis of RNA-Seq data from public databases suggested that most of the BoCALS genes exhibit tissue-specific expression patterns, indicating their potential roles in organ differentiation and development. QRT-PCR analysis elucidated a different expression level of BoCALS genes in response to Hyaloperonospora parasitica infection. Notably, BoCALS6 expression was significantly higher in resistant varieties compared to susceptible varieties and further up-regulated following H. parasitica infection, indicating its potential role in downy mildew resistance. This study presents the first comprehensive characterization of BoCALS gene family in B. oleracea and provides a foundation for further functional investigations into their roles in downy mildew resistance.

分类号：

  • 相关文献

[1]Genome-Wide Characterization of CaM/CML Gene Family in Cabbage (Brassica oleracea var. capitata): Expression Profiling and Functional Implications During Hyaloperonospora parasitica Infection. Yuankang Wu,Bin Zhang,Xuehui Yao,Limei Yang,Mu Zhuang,Honghao Lv,Yong Wang,Jialei Ji,Xilin Hou,Yangyong Zhang. 2025

[2]Genome-wide identification and analysis of the MADS-box gene family in apple. Tian, Yi,Dong, Qinglong,Ji, Zhirui,Chi, Fumei,Cong, Peihua,Zhou, Zongshan.

[3]Genome-wide identification of SWEET genes reveals their roles during seed development in peanuts. Yang Li,Mengjia Fu,Jiaming Li,Jie Wu,Zhenyang Shua,Tiantian Chen,Wen Yao,Dongxin Huai. 2024

[4]Genome-Wide Identification and Expression Profile Analysis of the NADPH Oxidase Gene Family in Avena sativa L.. Jiang, Qingxue,Zhou, Xinyue,Tang, Jun,Yi, Dengxia,Ma, Lin,Wang, Xuemin. 2025

[5]Molecular Evolution of Plant SULTR Proteins and Expression Analysis of HvSULTR Under Heat Stress in Barley. Chunmeng Zhu,Xuan Chen,Li Hao,Wessam A. Abdelrady,Tao Tong,Fenglin Deng,Fanrong Zeng,Zhong Hua Chen,Xiaojian Wu,Wei Jiang. 2025

[6]Identification and Molecular Mapping of the RsDmR Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish (Raphanus sativus L.). Xu Liang,Jiang Qiu-wei,Wang Yan,Gong Yi-qin,Wang Xian-li,Limera Cecilia,Liu Li-wang,Wu Jian. 2014

[7]Proteomic Analysis of the Resistant Responses of Two Vitis amurensis Cultivars to Plasmopara viticola Infections. Xu, Pei-lei,Liu, Ying-xue,Qin, Hong-yan,Ai, Jun,Fan, Shu-tian,Yang, Yi-ming,Zhao, Ying,Li, Xiao-yan,Li, Xiao-hong.

[8]Cloning and Characterization of a GmSAGT1 Gene Whose Transcriptional Expression Is Associated with the Level of Resistance to Soybean Downy Mildew. Sun, Wenli,Yin, Chao,Li, Mei,Zhang, Huaguang,Shi, Shuangfeng,Jia, Shirong,Wang, Zhixing,Liu, Yuhui,Sun, Wenli,Wu, Yuanhua,Wu, Qiao.

[9]Downy Mildew Resistance Identification And S.sr Molecular Marker Screening O f Different Grape Germplasm Resources. Zhao, H, Ju, YL, Jiang, JF, Min, Z, Fang, YL, Liu, CH. 2019

[10]Fine Mapping and Identification of a Candidate Gene of Downy Mildew Resistance, RPF2, in Spinach (Spinacia oleracea L.). Shuo Gao,Tiantian Lu,Hongbing She,Zhaosheng Xu,Helong Zhang,Zhiyuan Liu,Wei Qian. 2022

[11]A Whole-Genome Assembly for Hyaloperonospora parasitica, A Pathogen Causing Downy Mildew in Cabbage (Brassica oleracea var. capitata L.). Yuankang Wu,Bin Zhang,Shaobo Liu,Zhiwei Zhao,Wenjing Ren,Li Chen,Limei Yang,Mu Zhuang,Honghao Lv,Yong Wang,Jialei Ji,Fengqing Han,Yangyong Zhang. 2023

[12]Comparative Analysis of Transcriptomics and Metabolomics Reveals Defense Mechanisms in Melon Cultivars against Pseudoperonospora cubensis Infection. Ling, Yueming,Xiong, Xianpeng,Yang, Wenli,Liu, Bin,Shen, Yue,Xu, Lirong,Lu, Fuyuan,Li, Meihua,Guo, Yangdong,Zhang, Xuejun. 2024

[13]Fine mapping and identification of the downy mildew resistance gene BoDMR2 in Cabbage (Brassica oleracea L. var. capitata). Yuankang Wu,Bin Zhang,Limei yang,Mu zhuang,Honghao Lv,Yong wang,Jialei Ji,Xilin Hou,Yangyong Zhang. 2024

[14]Grapevine phyllosphere pan-metagenomics reveals pan-microbiome structure, diversity, and functional roles in downy mildew resistance. Jin, Jingyun,Wang, Xiangfeng,Zhang, Xuenan,Mei, Junjie,Zheng, Wei,Guo, Linling,Sun, Haisheng,Zhang, Lili,Liu, Chonghuai,Ye, Wenxiu,Guo, Li. 2025

[15]Identification Of Quantitative Trait Loci F.or Clubroot Resistance In B rassica Oleracea With The Use Of Brassica Snp Microarray. Peng, Lisha,Peng, Lisha,Peng, Lisha,Peng, Lisha,Zhou, Lili,Zhou, Lili,Zhou, Lili,Wei, Dayong,Wei, Dayong,Wei, Dayong,Song, Hongyuan,Song, Hongyuan,Song, Hongyuan,Ren, Xuesong,Ren, Xuesong,Ren, Xuesong,Qian, Wei,Qian, Wei,Li, Qinfei,Li, Qinfei,Li, Qinfei,Si, Jun,Si, Jun,Si, Jun,Mei, Jiaqin,Mei, Jiaqin. 2018

[16]Cytogenetic analysis of F-1, F-2 and BC, plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization. Wei, W. H.,Zhang, S. F.,Wang, L. J.,Li, J.,Chen, B.,Wang, Z.,Luo, L. X.,Fang, X. P.. 2007

[17]Assessing and broadening genetic diversity of a rapeseed germplasm collection. Wu, Jinfeng,Li, Feng,Xu, Kun,Cao, Guizhen,Chen, Biyun,Yan, Cuixin,Wang, Nian,Qiao, Jiangwei,Li, Jun,Li, Hao,Zhang, Tianyao,Song, Weiling,Wu, Xiaoming.

[18]Linkage map construction using InDel and SSR markers and QTL analysis of heading traits in Brassica oleracea var. capitata L.. Lv, Honghao,Wang, Qingbiao,Zhang, Yangyong,Yang, Limei,Fang, Zhiyuan,Wang, Xiaowu,Liu, Yumei,Zhuang, Mu,Lin, Yan,Yu, Hailong,Liu, Bo.

[19]Genetics and fine mapping of a yellow-green leaf gene (ygl-1) in cabbage (Brassica oleracea var. capitata L.). Liu, Xiao-ping,Yang, Chong,Han, Feng-qing,Fang, Zhi-yuan,Yang, Li-mei,Zhuang, Mu,Lv, Hong-hao,Liu, Yu-mei,Li, Zhan-sheng,Zhang, Yang-yong,Yang, Chong.

[20]Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea. Wang, XW,Lou, P,Bonnema, G,Yang, BJ,He, HJ,Zhang, YG,Fang, ZY. 2005

作者其他论文 更多>>