The Study on the Transformation and Selection of Insect-resistant Cotton Harboring Double-gene
文献类型: 外文期刊
作者: LI Fu-guang;GUO San-dui;LIU Chuan-liang;LI Feng -lian;CU I Hongzhi;ZHOU Yong;LI Xiu-lan
作者机构:
关键词: 双价抗虫基因;陆地棉;遗传转化;棉铃虫;抗性鉴定
期刊名称: 棉花学报
ISSN: 1002-7807
年卷期: 1999 年 02 期
页码:
收录情况: 北大核心 ; CSCD
摘要: 采用花粉管通道法,将人工合成的GFMCryIA和修饰的CpTI杀虫基因导入棉花优良品种(系)中棉所23、中棉所27、中9409和中90系。经田间选择和室内生物鉴定,已获得了转基因抗虫植株。依据生物鉴定结果,对所获得的抗虫材料进行了PCR分析,结果证明Bt和CpTI基因均整合于相应的抗虫材料染色体基因组中,通过自交选育,双价抗虫基因能够传递至ZGK9708抗虫材料R3代。抗虫鉴定表明,双价抗虫基因能够遗传,后代抗虫性稳定;在获得的R1代植株中,不同转化体对棉铃虫的抗性存在着差异。
分类号: S562.032
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