数字农科院2.0

A rapid and cost-effective approach for quantifying the content of genetically modified organism to facilitate the enforcement of labeling regulation

文献类型： 外文期刊

作者： Xiaoqing Yao;Jun Li;Danfeng Wan;Hongfei Gao;Yunjing Li;Fang Xiao;Shanshan Zhai;Gang Wu;Yuhua Wu

作者机构：

关键词： 2-ΔΔCt method;Genetically modified organism;Labeling;Rapid quantification;T-test

期刊名称： Microchemical Journal

ISSN： 0026-265X

年卷期： 2025 年 214 卷

页码：

收录情况： SCIE（2025版）

摘要： The current accurate quantitative technology, such as standard curve-based quantitative real time PCR (qPCR) and digital PCR (dPCR), is time-consuming and expensive, resulting in high costs for the implementation of the GMO labeling policy. This study proposed a rapid quantitative strategy that combines the 2-ΔΔCt method with the t-test of ΔCt values between the test samples and the reference control. This method allowed for an approximate estimation of the GMO content of test samples while identifying the GM events. GMO labeling or exempt for most samples can be determined based on the rapid estimation of GMO content and the t-test result of the ΔCt values between the test samples and the reference control. This approach streamlines the quantitative analysis process, makes the quantification more accessible and affordable, and supports compliance with GMO labeling regulations worldwide.

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