数字农科院2.0

Implication of Two Small Heat Shock Proteins in the Thermotolerance of Bradysia odoriphaga (Diptera: Sciaridae) Yang et Zhang

文献类型： 外文期刊

作者： Jiaxu Cheng;Huixin Zheng;Shuo Feng;Weiping Cao;Qingjun Wu;Jian Song

作者机构：

关键词： Bradysia odoriphaga Yang et Zhang;gene expression;heat stress;RNA interference;small heat shock proteins (sHsps)

期刊名称： Insects

ISSN： 2075-4450

年卷期： 2025 年 16 卷 11 期

页码：

收录情况： SCIE（2025版）

摘要： Bradysia odoriphaga Yang et Zhang damages roots of 30 plant species, resulting in >50% yield loss. Heat stress can not only affect the survival but also affect the expression of heat shock proteins of B. odoriphaga. In this study, two small heat shock protein genes, Hsp21.9 and Hsp22.3, were cloned from B. odoriphaga. The full-length cDNA sequences of BoHsp21.9 and BoHsp22.3 were 749 and 941 bp in length and contained a 588 and 594 bp open reading frame (ORF), encoding a protein of 196 and 198 amino acids with a calculated molecular weight of 21.9 and 22.3 kDa and an isoelectric point of 6.84 and 6.91. Phylogenetic tree analysis showed that BoHsp21.9 and BoHsp22.3 clustered into one branch with flies. qRT-PCR analyses indicated that BoHsp21.9 and BoHsp22.3 were expressed in all tested developmental stages and body segments, especially induced by heat stress. RNAi-mediated silencing of BoHsp21.9 and BoHsp22.3 significantly decreased the survival rate of fourth-instar larvae when exposed to 38 °C. This is the first study on small heat shock proteins in B. odoriphaga, and BoHsp21.9, and BoHsp22.3 play important roles in the molecular mechanism of B. odoriphaga to theromotolerance.

分类号：

  • 相关文献

[1]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[2]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

[3]Genome wide identification, classification and functional characterization of heat shock transcription factors in cultivated and ancestral cottons (Gossypium spp.). Abdul Rehman,Rana Muhammad Atif,Muhammad Tehseen Azhar,Zhen Peng,Hongge Li,Guangyong Qin,Yinhua Jia,Zhaoe Pan,Shoupu He,Abdul Qayyum,Xiongming Du. 2021

[4]Comprehensive Genome-Wide Analysis of Histone Acetylation Genes in Roses and Expression Analyses in Response to Heat Stress. Quanshu Wu,Qiuyue Huang,Huilin Guan,Xiaoni Zhang,Manzhu Bao,Mohammed Bendahmane,Xiaopeng Fu. 2022

[5]Comparative Transcriptome Analysis Of Differentially Expressed Genes In Bradysia Odoriphaga Yang Et Zhang (Diptera: Sciaridae) At Different Acute Stress Temperatures. Cheng, JX, Su, Q, Xia, JX, Yang, ZZ, Shi, CH, Wang, SL, Wu, QJ, Li, CN, Zhang, YJ. 2020

[6]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[7]cis-Regulatory variation affecting gene expression contributes to the improvement of maize kernel size. Li Y.-X., Lu J., He C., Wu X., Cui Y., Chen L., Zhang J., Xie Y., An Y., Liu X., Zhen S., Liu Y., Li C., Zhang D., Shi Y.-S., Song Y., Wang J., Li Y., Wang G., Fu J., Wang T.. 2022

[8]Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Wan, Pin-Jun.

[9]Effective inhibition of Japanese encephalitis virus replication by small interfering RNAs targeting the NS5 gene. Qi, Wen-Bao,Hua, Rong-Hong,Yan, Li-Ping,Tong, Guang-Zhi,Wu, Dong-Lai,Qi, Wen-Bao,Tong, Guang-Zhi,Zhang, Gui-Hong,Ren, Tao,Liao, Ming. 2008

[10]The effect of silencing arginine kinase by RNAi on the larval development of Helicoverpa armigera. Su, X. -F.,Lu, G. -Q.,Cheng, H. -M.,Liu, C. -X.,Liang, G. -M..

[11]Silencing in Apolygus lucorum of the olfactory coreceptor Orco gene by RNA interference induces EAG response declining to two putative semiochemicals. Zhu, Xiao-Qiang,Gu, Shao-Hua,Cui, Huan-huan,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhou, Jing-Jiang. 2014

[12]In vitro inhibition of transmissible gastroenteritis coronavirus replication in swine testicular cells by short hairpin RNAs targeting the ORF 7 gene. He, Lei,Zhang, Yan-ming,Dong, Ling-juan,Cheng, Min,Wang, Jing,Tang, Qing-hai,Wang, Gang,Tang, Qing-hai. 2012

[13]Knockdown of CDK2AP1 by RNA interference inhibits cell growth and tumorigenesis of human glioma. Xu, Yonggang,Wang, Zhi,Wang, Jie,Fu, Songbin. 2014

[14]Reduction of infectious bursal disease virus replication by shRNAs targeting the VP1 and VP2 genes driven by chicken U6 promoter. Wei Ouyang,Ma, Jin-rong,Jiang, Jie-yuan,Wang, Yong-shan,Wang, Yong-qiang,Qin, Li-ting,Wang, Xiao-mei,Fan, Hong-jie. 2013

[15]In vitro and in vivo evaluation of small interference RNA-mediated gynaecophoral canal protein silencing in Schistosoma japonicum. Cheng, Guofeng,Fu, Zhiqiang,Lin, Jiaojiao,Cai, Youmin,Shi, Yi,Zhou, Yuancong,Jin, Youxin. 2009

[16]RNAi in the striped stem borer, Chilo suppressalis, establishes a functional role for aminopeptidase N in Cry1Ab intoxication. Du, L. X.,Liu, C. X.,Han, L. Z.,Peng, Y. F.,Gong, L..

[17]Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus. Zhang, Shu-zhen,Wu, Su-ran,Yu, Nai-tong,Wang, Jian-hua,Liu, Zhi-xin,Kulye, Mahesh,Zeng, Hong-mei. 2012

[18]Role of non-structural protein 2 in the regulation of the replication of the porcine reproductive and respiratory syndrome virus in MARC-145 cells: Effect of gene silencing and over expression. Wang, Feng-Xue,Wen, Yong-Jun,Yang, Bo-Chao,Liu, Zhun,Shi, Xin Chuan,Leng, Xue,Song, Ni,Wu, Hua,Chen, Li-Zhi,Cheng, Shi-Peng.

[19]Effects of injecting cadherin gene dsRNA on growth and development in diamondback moth Plutella xylostella (Lep.: Plutellidae). Yang, Z. -X.,Wu, Q. -J.,Wang, S. -L.,Xu, B. -Y.,Chang, X. -L.,Zhu, G. -R.,Zhang, Y. -J.,Yang, Z. -X.,Wen, L. -Z..

[20]Chrysopa septempunctata (Neuroptera: Chrysopidae) Vitellogenin Functions Through Effects on Egg Production and Hatching. Liu, C.,Mao, J.,Zeng, F.,Liu, C..

作者其他论文 更多>>