数字农科院2.0

Enhancing bread wheat resilience to cadmium and drought stress: insights from physiological, morphological, and transcriptomic responses to biochar and 24-epibrassinolide application

文献类型： 外文期刊

作者： Haider, Fasih Ullah;Ain, Noor-ul-;Siddique, Kadambot H. M.;Farooq, Muhammad;Li, Yuelin

作者机构：

关键词： Cadmium toxicity;Biochar;24-epibrassinolide;Gene expression

期刊名称： BIOCHAR

ISSN： 2524-7972

年卷期： 2025 年 7 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 农林核心（2024版）

摘要： Drought and cadmium (Cd) stress threaten sustainable crop production, highlighting the need for resilent agricultural practices. Individual application of biochar (BC) and brassinosteroids (24-epibrassinolide; EBL) can mitigate drought and Cd stress. However, their synergistic effects on alleviating drought and Cd stress at transcriptomic level in wheat (Triticum aestivum L.) remain underexplored. This study investigated the combined impact of BC (B0 = 0% w/w and B1 = 5% w/w) and EBL (H0 = control and H1 = 10-6 M) on wheat physiology, biomass, and digital gene expression under Cd (30 ppm) and drought stress (D0 = 75% water holding capacity (WHC) and D1 = 35% WHC). Drought and Cd stress significantly reduced biomass and photosynthetic activity while increasing oxidative stress and Cd uptake. However, the combined application of BC and EBL treatments showed notable improvements: root fresh biomass, leaf area, and shoot fresh biomass were increased by 39.41%, 66.49%, and 78.25% under D0 and by 48.24%, 63.76%, and 73.49% under D1, respectively, compared to the control. Moreover, Cd uptake by wheat leaves decreased by 71.42% under D0 and 184.10% under D1 with BC and EBL combined application. Transcriptome analysis identified 6,174 differentially expressed genes linked to detoxification, carbon and nitrogen metabolism, and stress responses. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses highlighted metabolic processes and catalytic functions. Weighted gene co-expression network analysis revealed key modules for stress adaptation, including secondary metabolite biosynthesis and signaling pathways. Transcription factor profiling showed upregulation of AP2/ERF, MYB, and WRKY families in the combined BC and EBL treatments. qPCR validation of RNA-seq data confirmed significant changes in gene expression, with the nitrate transporter and photosystem II CP47 exhibiting increased expression levels by 53.60% and 29.66%, respectively, under BC + EBL treatment at optimal moisture, and 53.38% and 48.82% under drought stress. In contrast, heavy metal transporter genes PMPCB and YCF1 were downregulated, which correlated with a reduction in Cd uptake. Interestingly, the regression analysis demonstrated that Cd concentration in leaves negatively correlated with (dehydrin-/LEA group) and (cadmium tolerance factor). Overall, this study confirms that combining BC and EBL effectively mitigates Cd stress in drought-affected wheat, enhancing growth and resilience.

分类号：

  • 相关文献

[1]Alleviation of Cadmium Stress in Wheat through the Combined Application of Boron and Biochar via Regulating Morpho-Physiological and Antioxidant Defense Mechanisms. Sajjad Hussain,Muhammad Irfan,Abdul Sattar,Shabir Hussain,Sami Ullah,Tahira Abbas,Haseeb Ur-Rehman,Farukh Nawaz,Abdulrahman Al-Hashimi,Mohamed S. Elshikh,Mumtaz Cheema,Jianjun Yang. 2022

[2]24-epibrassinolide mitigates cadmium toxicity in rice plants by activating the plant detoxification system and regulating genes expression involved in Cd/Fe uptake and translocation. Qina Huang,Yan Zhang,Song Liu,Han Wang,Fangyuan Gao,Guosheng Shao. 2024

[3]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

[4]Field-based evaluation of sugarcane bagasse biochar for managing root-knot nematodes in tomato. Maria Fayyaz,Amjad Abbas,Usman Arshad,Farrukh Azeem,Faiz Ahmad Joyia,Abdelaziz Hajjaji,Muhammad Amjad Ali. 2025

[5]Glutamate Alleviates Cadmium Toxicity In Rice Via Suppressing Cadmium Uptake And Translocation. Li, Mei,Jiang, Meng,Jiang, Jie,Li, Shan,Huang, Jianzhong,Shu, Qingyao,Jiang, Meng,Huang, Jianzhong,Jiang, Meng,Tan, Yuanyuan,Li, Mei,Shu, Qingyao,Song, Shiyong,Li, Shan. 2020

[6]Brassinosteroids Improve Quality of Summer Tea (Camellia sinensis L.) by Balancing Biosynthesis of Polyphenols and Amino Acids. Li, Xin,Ahammed, Golam J.,Li, Zhi-Xin,Zhang, Lan,Wei, Ji-Peng,Shen, Chen,Yan, Peng,Zhang, Li-Ping,Han, Wen-Yan,Ahammed, Golam J.,Li, Zhi-Xin,Shen, Chen. 2016

[7]Exogenous 24-Epibrassinolide Alleviates The Detrimental E.ffects Of Suboptimal Root Z one Temperature In Cucumber Seedlings. Anwar, A, Di, QH, Yan, Y, He, CX, Li, YS, Yu, XC. 2019

[8]24-Epibrassinolide regulates volatile metabolic pathways to improve tomato flavor quality during postharvest storage. Haoran Liu,Yuanyuan Li,Zhiyong Shao,Lihong Liu,Hao Chen,Songwen Li,Fanliang Meng,Dongyi Liang,Changqing Zhu,Qiaomei Wang. 2023

[9]24-Epibrassinolide Promotes No3- And Nh4+ I.on Flux Rate And N rt1 Gene Expression In Cucumber Under Suboptimal Root Zone Temperature. Anwar, A, Li, YS, He, CX, Yu, XC. 2019

[10]24-Epibrassinolide Facilitates Adventitious Root Formation by Coordinating Cell-Wall Polyamine Oxidase- and Plasma Membrane Respiratory Burst Oxidase Homologue-Derived Reactive Oxygen Species in Capsicum annuum L.. Wen, Zhengyang,Chen, Zhifeng,Liu, Xinyan,Sun, Jingbo,Zhang, Feng,Zhang, Mengxia,Dong, Chunjuan. 2023

[11]Deciphering proteomic mechanisms explaining the role of glutathione as an aid in improving plant fitness and tolerance against cadmium-toxicity in Brassica napus L.. Mittra P.K.,Rahman M.A.,Roy S.K.,Kwon S.-J.,Yun S.H.,Kun C.,Zhou M.,Katsube-Tanaka T.,Shiraiwa T.,Woo S.-H.. 2024

[12]Chemotaxis of rhizosphere Pseudomonas sp. induced by foliar spraying of lanthanum reduces cadmium uptake by pakchoi. Wang, Meng,Yu, Lei,Wang, Jing,Qin, Luyao,Sun, Xiaoyi,Liu, Jiaxiao,Han, Yun,Chen, Shibao. 2025

[13]Nutrient-Element-Mediated Alleviation of Cadmium Stress in Plants: Mechanistic Insights and Practical Implications. Sun, Xichao,Zhang, Liwen,Gu, Yingchen,Wang, Peng,Liu, Haiwei,Qiang, Liwen,Huang, Qingqing. 2025

[14]cis-Regulatory variation affecting gene expression contributes to the improvement of maize kernel size. Li Y.-X., Lu J., He C., Wu X., Cui Y., Chen L., Zhang J., Xie Y., An Y., Liu X., Zhen S., Liu Y., Li C., Zhang D., Shi Y.-S., Song Y., Wang J., Li Y., Wang G., Fu J., Wang T.. 2022

[15]Biochar Suppresses Bacterial Wilt Of Tomato By Improving Soil Chemical Properties And Shifting Soil Microbial Community. Lu, Yang,Cai, Kunzheng,Tian, Jihui,Lin, Weipeng,Tian, Jihui,Cai, Kunzheng,Gao, Yang,Lu, Yang,Lin, Weipeng,Gao, Yang. 2019

[16]Effect Of Rice-Straw Biochar Application O.n Rice (Oryza Sativa) R oot Growth And Nitrogen Utilization In Acidified Paddy Soil. Anwar, Sumera,Chen, Huizhe,Ji, Guangmei,Zhu, Defeng,Xiang, Jing,Zhang, Yuping,Zhang, Yikai,Ji, Guangmei. 2018

[17]The Global Warming Potential Of S.traw-Return Can Be Reduced B y Application Of Straw-Decomposing Microbial Inoculants And Biochar In Rice-Wheat Production Systems. Liu, De Li,Ma, Yuchun,Yang, Bo,Liu, De Li,Schwenke, Graeme,Ma, Yuchun,Liu, De Li,Ma, Yuchun. 2019

[18]Effect Of Biochar On Growth, P.hotosynthetic Characteristics And Nutrient D istribution In Sugarcane. Liao, Fen,Yang, Litao,Huang, Dongliang,Huang, Dongliang,Xue, Jianjun,Yang, Liu,Yang, Liu,Yang, Liu,Li, Qiang,Li, Yangrui,Liao, Fen,Huang, Dongliang,Liao, Fen,Li, Yangrui,Li, Yangrui,Liao, Fen. 2019

[19]Biochar Alleviated The Salt Stress O.f Induced Saline Paddy S oil And Improved The Biochemical Characteristics Of Rice Seedlings Differing In Salt Tolerance. Huang, Jie,Zhu, Lianfeng,Zhao, Futao,Jin, Qianyu,Hussain, Sajid,Zhang, Junhua,Cao, Xiaochuang,Zhu, Chunquan,Bai, Zhigang. 2019

[20]The Effect Of Two Types O.f Biochars On The E fficacy, Emission, Degradation, And Adsorption Of The Fumigant Methyl Isothiocyanate. Liu, Xiaoman,Li, Jun,Yan, Dongdong,Wang, Qiuxia,Huang, Bin,Fang, Wensheng,Cao, Aocheng,Han, Dawei,Guo, Meixia. 2017

作者其他论文 更多>>