数字农科院2.0

The Response of the miRNA Profiles of the Thyroid Gland to the Artificial Photoperiod in Ovariectomized and Estradiol-Treated Ewes

文献类型： 外文期刊

作者： Zizhen Ren;Wei Wang;Xiaoyun He;Mingxing Chu

作者机构：

关键词： miRNA;photoperiod;sheep;thyroid gland

期刊名称： Animals

ISSN： 2076-2615

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： The photoperiod has been considered to be a key environmental factor in sheep reproduction, and some studies have shown that the thyroid gland plays an important role in mammalian reproduction, but the molecular mechanism is still unclear. In this study, we used the artificial-light-controlled, ovariectomized, and estradiol-treated model (OVX + E2 model); healthy and consistent 2–3-year-old Sunite multiparous ewes were collected; and thyroids were collected for testing, combined with RNA-seq technology and bioinformatics analysis, to analyze the effects of different photoperiods (long photoperiod treatment for 42 days, LP42; short photoperiod treatment for 42 days, SP42; SP42 transferred to LP42, SPLP42) on the variations in the miRNA profiles of the thyroid gland. A total of 105 miRNAs were differentially expressed in the thyroid gland, most of which were new miRNAs. Through GO and KEGG enrichment analysis, the results showed that the photoperiod response characteristics of Sunite ewes were affected by Olfactory transduction, Wnt signaling pathways, and Apelin signaling pathways. A different illumination time may have a certain influence on the downstream of these pathways, which leads to the change in animal estrus state. In addition, lncRNA-mRNA-miRNA network analysis revealed the target binding sites of identified miRNAs in DE-circRNA and DE-mRNA, such as Novel_369, Novel_370, Novel_461, and so on. The results of this study will provide some new insights into the function of miRNA and the changes in sheep thyroid glands under different photoperiods.

分类号：

  • 相关文献

[1]Transcriptome Analysis Revealed Long Non-Coding RNAs Associated with mRNAs in Sheep Thyroid Gland under Different Photoperiods. Wang W.,He X.,Di R.,Wang X.,Chu M.. 2022

[2]Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes. Cheng Chang,Xiaoyun He,Ran Di,Xiangyu Wang,Miaoceng Han,Chen Liang,Mingxing Chu. 2023

[3]Comparative Transcriptomics Reveals the Key lncRNA and mRNA of Sunite Sheep Adrenal Gland Affecting Seasonal Reproduction. Xiaolong Du,Xiaoyun He,Qiuyue Liu,Ran Di,Qingqing Liu,Mingxing Chu. 2022

[4]Identification of Photoperiod-Induced LncRNAs and mRNAs in Pituitary Pars Tuberalis of Sheep. Qing Xia,Mingxing Chu,Xiaoyun He,Xiaosheng Zhang,Jinlong Zhang,Xiaofei Guo,Ran Di. 2021

[5]Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries). Xiaolong Du,Xiaoyun He,Qingqing Liu,Qiuyue Liu,Ran Di,Mingxing Chu. 2022

[6]Screening of Differentially Expressed Genes and miRNAs in Hypothalamus and Pituitary Gland of Sheep under Different Photoperiods. Qingqing Liu,Ran Di,Chunhuan Ren,Xiaoyun He,Xiangyu Wang,Qing Xia,Mingxing Chu,Zijun Zhang. 2022

[7]Photoperiod Induces the Epigenetic Change of the GNAQ Gene in OVX+E2 Ewes. Wei Wang,Xiaolong Du,Mingxing Chu,Xiaoyun He. 2023

[8]Integrated analysis of differential expression profile of miRNA in the uterus of seasonal estrus sheep. Yang, Fan,Liu, Jiannan,La, Yongfu,Di, Ran,He, Xiaoyun,Wang, Pingqing,Liu, Yufang,Chu, Mingxing. 2025

[9]Photoperiodic responsiveness in the DNA methylation and gene expression in the hypothalamus of ovariectomized and estradiol-treated ewes. Xiaoyun He,Ran Di,Xiangyu Wang,Xiaofei Guo,Xiaosheng Zhang,Jinlong Zhang,Qiuyue Liu,Mingxing Chu. 2025

[10]Characterization and comparative profiling of ovarian microRNAs during ovine anestrus and the breeding season. Di, Ran,He, Jianning,Liu, Qiuyue,Cao, Guiling,Wang, Jinxin,Yang, Zhimin,Ge, Ying,Chu, Mingxing,Song, Shuhui,Tian, Dongmei,Sun, Min,Zhao, Wenming,Liang, Xiaojun,Ma, Qing,Wang, Jiandong. 2014

[11]Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity. Miao, Xiangyang,Qingmiao Luo Xiaoyu Qin. 2015

[12]Genome-wide analysis reveals the differential regulations of mRNAs and miRNAs in Dorset and Small Tail Han sheep muscles. Miao, Xiangyang,Luo, Qingmiao,Qin, Xiaoyu.

[13]Epigenetic Regulation of miR-25 and Lnc107153 on Expression of Seasonal Estrus Key Gene CHGA in Sheep. Ran Di,Yekai Fan,Xiaoyun He,Qiuyue Liu,Xiangyu Wang,Yiming Gong,Joram Mwashigadi Mwacharo,Caihong Wei,Yufang Liu,Mingxing Chu. 2023

[14]Global DNA Methylation, miRNA, and mRNA Profiles in Sheep Skeletal Muscle Promoted by Hybridization. Chen, Bowen,Yue, Yaojing,Li, Jianye,Yuan, Chao,Guo, Tingting,Zhang, Dan,Liu, Jianbin,Yang, Bohui,Lu, Zengkui. 2023

[15]Integrated analysis of microRNA and mRNA interactions regulating fecundity in the ovaries of two distinct sheep breeds. Salsabeel Yousuf,Waqar Afzal Malik,Hui Feng,Tianyi Liu,Lingli Xie,Xiangyang Miao. 2025

[16]赖氨酸对绵羊GHR基因表达的影响(英文). 李建升,程胜利,韩向敏,冯瑞林,岳耀敬,杨博辉,刘建斌. 2009

[17]基于ssRNA-seq研究差异基因、NATs对藏羊高原低氧适应的调控. 岳耀敬,冯瑞林,王天翔,郭婷婷,李桂英,孙晓萍,刘建斌,李文辉,牛春娥,郭健,李范文,杨博辉. 2015

[18]Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with FecB BB genotype. Chang, Cheng,He, Xiaoyun,Di, Ran,Wang, Xiangyu,Han, Miaoceng,Liang, Chen,Chu, Mingxing. 2024

[19]Transcriptome Analysis Reveals Differentially Expressed circRNAs Associated with Fecundity in Small-Tail Han Sheep Thyroid with Different FecB Genotypes. Chang C.,He X.,Di R.,Wang X.,Han M.,Liang C.,Chu M.. 2024

[20]猪流行性腹泻病毒感染猪小肠上皮细胞miRNA表达谱分析及验证. 张宸语,陈佳宁,温建新,刘光亮. 2018

作者其他论文 更多>>