数字农科院2.0

Novel ACE2 binding in bat merbecoviruses expands potential host range

文献类型： 外文期刊

作者： Xiaoguang Zhang; Xing Ma; Ye Chen2; Yabo You; Xin-Xin Li;Jie Chen; Shuhan Kong; Dawei Guo;Xiaoling Li;Michael Veit; Xiaofeng Zhai;Jin Tian

关键词： ACE2; merbecoviruses; bat

期刊名称： PLoS Pathogens

ISSN： 1553-7366

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Coronaviruses often cross species barriers, with receptor binding dictating their host range and zoonotic potential. Merbecoviruses, such as MERS-CoV, typically utilize DPP4 as their receptor, whereas Sarbecoviruses, like SARS-CoV, rely on ACE2. This study explores the receptor usage of four merbecoviruses identified in Vespertilionidae bats: HKU5, BtVs-SC2013, HKU25, and P. khulii-2011. Our findings reveal species-specific binding to bat ACE2: HKU5 binds exclusively to Pipistrellus abramus ACE2, P. khulii-2011 interacts solely with Murina aurata ACE2, BtVs-SC2013 recognizes ACE2 from Murina aurata and Myotis myotis, and HKU25 displays the broadest binding range. Beyond bats, BtVs-SC2013 binds to mink ACE2, while HKU25 interacts with both mink and pangolin ACE2, hinting at potential intermediate hosts for cross-species transmission. We also elucidated the mechanism behind HKU5’s selective binding preference for P. abramus ACE2. Structural analysis and mutagenesis revealed that a carbohydrate attached at position 329 play a crucial role. Introducing the N-glycosylation site into P. abramus ACE2 eliminated binding, while its removal from P. pipistrellus ACE2, combined with two additional mutations, restored it. Moreover, we pinpointed key residues in mink ACE2 essential for binding the receptor-binding domain (RBD) of BtVs-SC2013 and HKU25. These findings illuminate the receptor usage and host specificity of bat merbecoviruses, enhancing our understanding of their potential for cross-species transmission and adaptation.

分类号：

  • 相关文献

[1]新冠病毒SARS-CoV-2的感染机制研究进展. 鲁荣光,武婧,白雪,刘维全. 2020

[2]Molecular Docking and Dynamics Simulation Studies of Ginsenosides with SARS-CoV-2 Host and Viral Entry Protein Targets. Pang Shifeng,Vinothini Boopathi,Mohanapriya Murugesan,Ramya Mathiyalagan,Jong Chan Ahn,Chen Xiaolin,Dong Uk Yang,Gi Young Kwak,Byoung Man Kong,Deok Chun Yang,Se Chan Kang,Zhang Hao. 2022

作者其他论文 更多>>