数字农科院2.0

NbWRKY57 activates helper nucleotide-binding leucine-rich repeat receptor transcription to promote TNL-mediated effector-triggered immunity in Nicotiana benthamiana

文献类型： 外文期刊

作者： Jipeng Xie;Yin Yang;Hanling Wang;Tianxue Song;Qiangsheng Yu;Yuejuan Zeng;Changzhen Liu;Susheng Song;Tiancong Qi

作者机构：

关键词： TNL immune receptor; NRG1; transcriptional regulation; WRKY57

期刊名称： Plant Physiology

ISSN： 1532-2548

年卷期： 2025 年 199 卷 3 期

页码：

收录情况： SCIE（2025版）

摘要： Plants deploy intracellular nucleotide-binding leucine-rich repeat receptors (NLRs) to detect pathogenic effectors and trigger effector-triggered immunity (ETI). Helper NLRs, such as N required gene 1 (NRG1), act downstream of sensor NLRs to transduce immune signals. NRG1s have been implicated to oligomerize to form Ca2⁺-permeable channels at the plasma membrane, playing a critical role in TIR-NLR (TNL)-mediated ETI. Although its biochemical function is well understood, the transcriptional regulation of NRG1 remains unclear. Here, we show that ETI activation by various TNLs upregulates NbNRG1 expression in Nicotiana benthamiana. We identify NbWRKY57 as a key transcriptional activator that directly binds the W-box motif in the NbNRG1 promoter. Knockdown or knockout of NbWRKY57 compromises TNLRoq1-mediated hypersensitive response (HR) cell death and pathogen resistance, while its overexpression restores mutant HR phenotypes, underscoring its positive regulatory role in TNL-mediated ETI. Moreover, NbWRKY57 is essential not only for TNLRoq1-mediated ETI but also for ETI mediated by other TNLs, including N and RPP1. We further demonstrate that calcium signaling contributes to NbNRG1 activation and TNL-mediated ETI. Together, these findings uncover a previously unknown regulatory mechanism controlling helper NLR transcription and provide insights into the transcriptional activation of NRG1 during ETI.

分类号：

  • 相关文献

[1]Histone Tales: Lysine Methylation, A Protagonist In Arabidopsis Development. Wu, Hualing,Cheng, Kai,Ouellette, Luc,Chu, Liutian,Zhuang, Feng,Liu, Jin,Wu, Hualing,Zhuang, Feng,Luo, Ming,Yang, Chao,Chu, Liutian,Cheng, Kai,Xu, Yingchao,Zhou, Xiaochen,Zhou, Xiaochen,Liu, Jin,Charron, Jean-Benoit,Niu, Longjian,Xu, Yingchao. 2020

[2]Anthocyanin Biosynthesis Regulation Of Dhmyb2 A.nd Dhbhlh1 In Dendrobium H ybrids Petals. Yang, Guangsui,Qiu, Flan,Li, Chonghui,Yin, Junmei,Yin, Junmei,Yang, Guangsui,Li, Chonghui,Huang, Mingzhong,Ding, Ling,Ding, Ling,Huang, Surong,Huang, Surong,Huang, Mingzhong. 2017

[3]Molecular Mechanisms Determining The Differential Accumulation Of Carotenoids In Plant Species And Varieties. Lu, Pengjun,Grierson, Donald,Grierson, Donald,Fu, Xiumin,Luan, Yuting,Xu, Changjie. 2020

[4]Crispr Editing In Biological And B.iomedical Investigation. Huang, JJ, Wang, YF, Zhao, JG. 2018

[5]Dry Storage Improves The Vase Quality Of Cut Peony By Increasing Water Uptake Efficiency Through Aquaporins Regulation. Xue, JQ, Huang, Z, Wang, SL, Xue, YQ, Ren, XX, Zeng, XL, Zhang, XX. 2020

[6]Dissecting Conserved Cis-Regulatory Modules Of G.lu-1 Promoters Which Confer T he Highly Active Endosperm-Specific Expression Via Stable Wheat Transformation. Li, JH, Wang, K, Li, GY, Li, YL, Zhang, Y, Liu, ZY, Ye, XG, Xia, XC, He, ZH, Cao, SH. 2019

[7]Tobacco Transcription Factor Ntbhlh123 Confers T.olerance To Cold Stress B y Regulating The Ntcbf Pathway And Reactive Oxygen Species Homeostasis. Zhao, Q, Xiang, XH, Liu, D, Yang, AG, Wang, YY. 2018

[8]Exogenous Melatonin Alleviates Cadmium Uptake And Toxicity In Apple Rootstocks. He, JL, Zhuang, XL, Zhou, JT, Sun, LY, Wan, HX, Li, HF, Lyu, DG. 2020

[9]SET DOMAIN GROUP 721 protein functions in saline–alkaline stress tolerance in the model rice variety Kitaake. Yutong Liu,Xi Chen,Shangyong Xue,Taiyong Quan, Di Cui, Longzhi Han,Weixuan Cong,Mengting Li, Dae-Jin Yun, Bao Liu,Zheng-Yi Xu. 2021

[10]Transcriptome regulation of carotenoids in five flesh-colored watermelons (Citrullus lanatus). Yuan Pingli,Umer Muhammad Jawad,He Nan,Zhao Shengjie,Lu Xuqiang,Zhu Hongju,Gong Chengsheng,Diao Weinan,Gebremeskel Haileslassie,Kuang Hanhui,Liu Wenge. 2021

[11]The inhibitory mechanism of methyl jasmonate on Aspergillus flavus growth and aflatoxin biosynthesis and two novel transcription factors are involved in this action. Xu Li,Yaoyao Ren,Jin Jing,Yiran Jiang,Qingli Yang,Shujuan Luo,Fuguo Xing. 2021

[12]The Light-Induced WD40-Repeat Transcription Factor DcTTG1 Regulates Anthocyanin Biosynthesis in Dendrobium candidum. Ning Jia,Jingjing Wang,Yajuan Wang,Wei Ye,Jiameng Liu,Jinlan Jiang,Jing Sun,Peipei Yan,Peiyu Wang,Fengzhong Wang,Bei Fan. 2021

[13]Ethylene Response Factor Nterf91 Positively R.egulates Alkaloid Accumulations In T obacco (Nicotiana Tabacum L.). Sui, XY, Zhang, HB, Song, ZB, Gao, YL, Li, WZ, Li, MY, Zhao, L, Li, YP, Wang, BW. 2019

[14]Reversible Histone H2B Monoubiquitination Fine-Tunes A.bscisic Acid Signaling And D rought Response In Rice. Ma, SQ, Tang, N, Li, X, Xie, YJ, Xiang, DH, Fu, J, Shen, JQ, Yang, J, Tu, HF, Li, XH, Hu, HH, Xiong, LZ. 2019

[15]DcTT8, a bHLH transcription factor, regulates anthocyanin biosynthesis in Dendrobium candidum. Ning Jia,Jing-Jing Wang,Jiameng Liu,Jinlan Jiang,Jing Sun,Peipei Yan,Yufeng Sun,Peiyu Wan,Wei Ye,Bei Fan. 2021

[16]Sbbr/Sbba, An Important Arpa/Afsa-Like System, R.egulates Milbemycin Production In S treptomyces Bingchenggensis. He, Hairong,Ye, Lan,Li, Chuang,Wang, Haiyan,Li, Chuang,Zhang, Yanyan,Guo, Xiaowei,Xiang, Wensheng,Wang, Xiangjing,He, Hairong,Ye, Lan,Xiang, Wensheng. 2018

[17]Molecular regulation of zinc deficiency responses in plants. Zeng Houqing,Wu Haicheng,Yan Feng,Yi Keke,Zhu Yiyong. 2021

[18]Transcriptome And Metabolome Reveal Redirection Of Flavonoids In A White Testa Peanut Mutant. Wan, LY, Lei, Y, Yan, LY, Liu, Y, Pandey, MK, Wan, X, Varshney, RK, Fang, JH, Liao, BS. 2020

[19]SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake. Liu, Yutong,Chen, Xi,Xue, Shangyong,Quan, Taiyong,Cui, Di,Han, Longzhi,Cong, Weixuan,Li, Mengting,Yun, Dae-Jin,Liu, Bao,Xu, Zheng-Yi. 2021

[20]The landscape of lncRNAs in Cydia pomonella provides insights into their signatures and potential roles in transcriptional regulation. Longsheng Xing,Yu Xi,Xi Qiao,Cong Huang,Qiang Wu,Nianwan Yang,Jianyang Guo,Wanxue Liu,Wei Fan,Fanghao Wan,Wanqiang Qian. 2021

作者其他论文 更多>>