数字农科院2.0

The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella

文献类型： 外文期刊

作者： Sun, Pei;Wang, Chaoyue;Xie, Fujie;Chen, Linlin;Zhang, Yuanyuan;Tang, Xinming;Hu, Dandan;Gao, Yang;Zhang, Ning;Hao, Zhenkai;Yu, Yonglan;Suo, Jingxia;Suo, Xun;Liu, Xianyong

作者机构：

关键词： Eimeria tenella;salinomycin;drug resistance;point mutation

期刊名称： VETERINARY RESEARCH

ISSN： 0928-4249

年卷期： 2024 年 55 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches. Whole-genome sequencing of salinomycin-resistant Eimeria tenella isolates revealed single nucleotide polymorphisms (SNPs), with 12 candidate genes harboring nonsynonymous mutations identified. To confirm the candidate gene responsible for conferring salinomycin resistance, we leveraged reverse genetic strategies and identified a key amino acid substitution (F204S) in adrenodoxin oxidoreductase (EtADR), which markedly reduced susceptibility to salinomycin. Our results elucidate the complex interactions among salinomycin resistance, parasite fitness, point mutations, and the structure of EtADR, laying the foundation for future studies on drug resistance in Eimeria and contributing to the development of targeted control strategies.

分类号：

  • 相关文献

[1]Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase from Eimeria tenella. Huang, Wenhao,Zhu, Shunhai,Chen, Ting,Zhao, Qiping,Dong, Hui,Huang, Bing,Yao, Yawen,Liu, Zhan,Yu, Yu,Han, Hongyu. 2022

[2]Label-free quantitative proteomic analysis of ethanamizuril-resistant versus -sensitive strains of Eimeria tenella. Peipei Cheng,Chunmei Wang,Lifang Zhang,Chenzhong Fei,Yingchun Liu,Mi Wang,Keyu Zhang,Xiaoyang Wang,Feng Gu,Feiqun Xue. 2022

[3]Distinct non-synonymous mutations in cytochrome b highly correlate with decoquinate resistance in apicomplexan parasite Eimeria tenella. Zhenkai Hao,Junmin Chen,Pei Sun,Linlin Chen,Yuanyuan Zhang,Wenxuan Chen,Dandan Hu,Feifei Bi,Zhenyan Han,Xinming Tang,Jingxia Suo,Xun Suo,Xianyong Liu. 2023

[4]Integrated application of transcriptomics and metabolomics provides insight into the mechanism of Eimeria tenella resistance to maduramycin. Huanzhi Zhao,Hui Dong,Qiping Zhao,Shunhai Zhu,Liushu Jia,Sishi Zhang,Qian Feng,Yu Yu,Jinwen Wang,Bing Huang,Hongyu Han. 2024

[5]Molecular characterization and functional analysis of Eimeria tenella ankyrin repeat-containing protein. Huilin Guo,Qiping Zhao,Haixia Wang,Shunhai Zhu,Hui Dong,Xinrui Xie,Lihui Wang,Lang Chen,Hongyu Han. 2024

[6]Molecular characterization of methionine aminopeptidase1 from Eimeria tenella. Ke Xiao,Qiping Zhao,Haixia Wang,Shunhai Zhu,Hui Dong,Bing Huang,Hongyu Han. 2023

[7]Molecular characterization and analysis of the drug resistance-associated protein phosphoglycerate kinase of Eimeria tenella. Yu, Yu,Huang, Wenhao,Zhao, Qiping,Zhu, Shunhai,Dong, Hui,Han, Hongyu. 2024

[8]Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella. Yu Yu,Guo Huilin,Liujia Li,Guiquan Guan,Qiping Zhao,Shunhai Zhu,Jinwen Wang,Fanghe Zhao,Hui Dong,Hongyu Han. 2025

[9]Effects of salinomycin and ethanamizuril on the three microbial communities in vivo and in vitro. Cheng, Xiaolei,Zheng, Haihong,Wang, Chunmei,Wang, Xiaoyang,Fei, Chenzhong,Zhou, Wen,Zhang, Keyu. 2022

[10]Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum. Qiu, Lingling,Ma, Teng,Chang, Guobin,Guo, Xiaomin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[11]Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING. Li, Wenjie,Zhao, Baocun,Li, Wenjie,Guo, Huijun,Xie, Yongdun,Zhao, Linshu,Gu, Jiayu,Zhao, Shirong,Liu, Luxiang,Wang, Yongbin,Wang, Guangjin.

[12]Artificial selection for determinate growth habit in soybean. Lee, Rian,McClean, Phillip E.,Lee, Rian,McClean, Phillip E.,Wang, Xiaobo,Li, Yinghui,Qiu, Lijuan,Tian, Zhixi,Ma, Jianxin,Specht, James E.,Nelson, Randall L.,Nelson, Randall L..

[13]Changing a conserved amino acid in R2R3-MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis. Zhou, Meiliang,Sun, Zhanmin,Wang, Chenglong,Tang, Yixiong,Wu, Yanmin,Wang, Chenglong,Shao, Jirong,Zhang, Xinquan,Zhu, Xuemei.

[14]Continuing evolution of canine parvovirus in China: Isolation of novel variants with an Ala5Gly mutation in the VP2 protein. Wang, Jianke,Lin, Peng,Zhao, Hang,Cheng, Yuening,Zhu, Hongwei,Wu, Hua,Cheng, Shipeng,Cheng, Yuening,Jiang, Zhong.

[15]D27E mutation of VTC1 impairs the interaction with CSN5B and enhances ascorbic acid biosynthesis and seedling growth in Arabidopsis. Li, Shenghui,Yu, Yanwen,Wang, Fengru,Dong, Jingao,Li, Shenghui,Wang, Juan,Yu, Yanwen,Huang, Rongfeng,Wang, Juan,Huang, Rongfeng.

[16]A conserved lysine/arginine-rich motif is essential for the autophagic degradation of potyviral 6K1 protein and virus infection. Hu, Weiyao,Deng, Changhui,Qin, Li,Liu, Peilan,Wang, Linxi,Wang, Xaioqing,Shi, Wei,Aziz, Asma,Li, Fangfang,Cheng, Xiaofei,Wang, Aiming,Dai, Zhaoji,Xiang, Xiaohua,Cui, Hongguang. 2025

[17]Resistance to drug by different isolates Trypanosoma evansi in China. Zhou, JL,Shen, J,Liao, DJ,Zhou, YZ,Lin, JJ. 2004

[18]Changes in antimicrobial resistance among Salmonella enterica subspecies enterica serovar Pullorum isolates in China from 1962 to 2007. Pan, Zhiming,Wang, Xiaoquan,Zhang, Xiaoming,Geng, Shizhong,Chen, Xiang,Pan, Weijuan,Cong, Qiuxia,Jiao, Xinan,Liu, Xiufan,Liu, Xuexian.

[19]Prevalence and Antimicrobial Resistance of Salmonella enterica Serovar Indiana in China (1984-2016). Gong, J.,Gong, J.,Wang, C.,Kelly, P.,Wang, C.,Wang, C..

[20]Characterization of avian pathogenic Escherichia coli isolated in eastern China. Dou, Xinhong,Gong, Jiansen,Xu, Ming,Shen, Haiyu,Zhang, Di,Liu, Jiasheng,Zou, Jianmin,Dou, Xinhong,Gong, Jiansen,Shen, Haiyu,Zou, Jianmin,Han, Xiangan,Zhuang, Linlin. 2016

作者其他论文 更多>>