数字农科院2.0

Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice

文献类型: 外文期刊

作者: Wang Tao;Lou Lijuan;Li Zeyu;Shang Lianguang;Wang Quan

作者机构:

关键词: Plant development;Pollen viability;Protein prenylation;Rice

期刊名称: Rice Science

ISSN: 1672-6308

年卷期: 2021 年 28.0 卷 6.0 期

页码:

收录情况: JCR(2021版) ; CSCD(2020-2021年度) ; 农林核心(2020版) ; 科技核心(2020版)

摘要: Protein prenylation plays a crucial role in plant development and stress response. We report the function of prenyltransferase α-subunit in rice. Protein-protein interactions showed that the farnesyl- transferase (OsPFT)/geranylgeranyltransferase-I (OsPGGT I-α) protein interacted together with OsPFT-β and OsPGGT I-β. The α- and β-subunits of OsPFT formed a heterodimer for the transfer of a farnesyl group from farnesyl pyrophosphate to the CaaX-box-containing peptide N-dansyl-GCVLS. Furthermore, the tissue expression patterns of the OsPFT and OsPGGT I subunits were similar, and these subunits were localized in the cytoplasm and nucleus. Moreover, OsPFT/OsPGGT I-α-deletion homozygous rice mutants had a lethal phenotype, and the heterozygous mutants exhibited reduced pollen viability. These results indicated that prenylation plays an important role in rice development.

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