数字农科院2.0

Draft genome sequence analysis of a mcr-1-producing Klebsiella pneumoniae ST661 isolated from a mink in China

文献类型： 外文期刊

作者： Na Sun;Yilin Luo;Yaru Zhao;Guisheng Wang;Li Guo;Liming Liu;Zhihao San;Cuiqing Zhao;Yuening Cheng;Qiang Chen

作者机构：

关键词： Klebsiella pneumoniae;mcr-1;Mink;Multidrug resistance

期刊名称： Journal of Global Antimicrobial Resistance

ISSN： 2213-7165

年卷期： 2024 年 37 卷

页码：

收录情况： SCIE（2024版）

摘要： Objectives: Klebsiella pneumoniae is a major opportunistic pathogen that is a member of the Enterobacteriaceae. Klebsiella pneumoniae causes pneumonia in mink and has become the primary infectious disease that limits mink farming. In this study, we report the draft genome sequence of a multidrug-resistant (MDR) strain of K. pneumoniae that harbours the mcr-1 gene isolated from a mink in China. Methods: The agar microdilution method was used to determine the minimum inhibitory concentration of the strain. The entire genomic DNA was sequenced using an Illumina MiSeq platform. A multilocus sequence type (MLST) and a core genome SNP phylogenetic tree analysis with a heatmap of the resistance genes and virulence genes were performed. Results: The size of the genome was 5451.826 kb, and it included one chromosome and one plasmid. The draft genome of K. pneumoniae indicated that the isolate was a member of MLST 661. Four types of virulence genes were detected. The results of antimicrobial susceptibility testing showed multiple drug resistance, and 17 resistance genes were identified. Conclusion: The genome sequence reported in this study will help to reveal the key role of antibiotic resistance and pathogenic mechanisms. It will provide useful information for the role of mobile genetic elements in the adaptive translocation and spread of antimicrobial resistance.

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