数字农科院2.0

Genome-wide identification and analysis of the abiotic stress responsiveness of the heat shock protein 90 gene family in Medicago sativa L

文献类型： 外文期刊

作者： Liu, Hao;Zhang, Yuqi;Li, Xianyang;Zhao, Li;Ma, Xinyue;He, Fei;Li, Mingna;Wang, Xue;Long, Ruicai;Kang, Junmei;Yang, Qingchuan;Chen, Lin

作者机构：

关键词： Medicago sativa L.;MsHSP90 gene family;Genome-wide analysis;Tissue-specific expression;Abiotic stress response

期刊名称： BMC PLANT BIOLOGY

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： BackgroundHeat shock protein 90 (HSP90) is a highly conserved class of cellular chaperone proteins that are widely present in plants and are involved in the response to various biotic and abiotic stresses. The HSP90 gene has been extensively studied across numerous species, and several investigations on alfalfa (Medicago sativa L.) have demonstrated that HSP90 gene is implicated in plant-pathogen interactions. However, the HSP90 gene has not been fully identified in alfalfa.ResultsIn this study, the HSP90 genes in alfalfa were identified and characterized throughout the genome. A total of 29 MsHSP90s were identified from the alfalfa Xinjiangdaye genome and were categorized into three subgroups, I, II, and III, with 15, 9, and 5 members in each group, respectively. The MsHSP90 family members in each subgroup have similar intron and exon structures, and motif analysis revealed that the MsHSP90 gene family is highly conserved. Analysis of RNA-seq data of MsHSP90 genes in different tissues (roots, elongated stems, pre-elongated stems, leaves, flowers, and nodules) revealed that the expression of three MsHSP90 genes (MsHSP90-4, MsHSP90-14, MsHSP90-18) had tissue specificity and that 17 MsHSP90 genes were expressed in all six tissues, but their expression levels varied significantly. Analysis of MsHSP90 RNA-seq data under three stresses (salt, drought, cold) revealed that four MsHSP90 genes (MsHSP90-10, MsHSP90-11, MsHSP90-16, and MsHSP90-27) respond to all three stresses. In this study, the HSP90 gene family of alfalfa was comprehensively identified and analyzed, and the candidate genes related to adverse stress conditions were identified by analyzing RNA-seq data.ConclusionThe findings of this study provide critical information for further analysis of the HSP90 gene function in alfalfa, laying a foundation for enhancing its stress resistance.

分类号：

  • 相关文献

[1]Genome-wide identification and analysis of abiotic stress responsiveness of the mitogen-activated protein kinase gene family in Medicago sativa L.. Hao Liu,Xianyang Li,Fei He,Mingna Li,Yunfei Zi,Ruicai Long,Guoqing Zhao,Lihua Zhu,Ling Hong,Shiqing Wang,Junmei Kang,Qingchuan Yang,Lin Chen. 2024

[2]Molecular Characteristics and Expression Patterns of Carotenoid Cleavage Oxygenase Family Genes in Rice (Oryza sativa L.). Hanjing Dai,Hao Ai,Yingrun Wang,Jia Shi,Lantian Ren,Jieqin Li,Yulu Tao,Zhaoshi Xu,Jiacheng Zheng. 2024

[3]Comprehensive profiling of Bcl-2-associated athanogene (BAG) genes and their genetic potential role under cold stress in Cotton. Aamir Ali Abro,Cong Sun,Mubashir Abbas,Qiankun Liu,Zheng Jie,Yanchao Xu,Yuqing Hou,Zhongli Zhou,Rashid Iqbal,Fang Liu,Xiaoyan Cai. 2025

[4]Molecular cloning and functional analysis of NAC family genes associated with leaf senescence and stresses in Gossypium hirsutum L.. Shah, Syed Tariq,Pang, Chaoyou,Hussain, Anwar,Fan, Shuli,Song, Meizhen,Zamir, Roshan,Yu, Shuxun.

[5]Genomic survey of NPF and NRT2 transporter gene families in five inbred maize lines and their responses to pathogens infection. Xinyao Xia, Qiuhe We, Chunxia Xiao, Yiping Ye, Zhiqiang Li, Cécile Marivingt-Mounir, Jean-François Chollet, Wende Liu, Hanxiang Wu. 2023

[6]Expression Profiles and Characteristics of Apple lncRNAs in Roots, Phloem, Leaves, Flowers, and Fruit. Wang, Dajiang,Gao, Yuan,Sun, Simiao,Li, Lianwen,Wang, Kun. 2022

[7]Characterization and Expression Profiling of Orphan Genes in Rapeseed (Brassica napus) Provide Insights into Tissue Development and Cold Stress Adaptation. Hong Lang,Yuting Zhang,Baofeng Wang,Kexin Li,Mingliang Jiang. 2025

[8]Comprehensive Characterization of Long Non-Coding RNAs in Porcine Tissues: Expression Patterns and Functional Insights During Oocyte Development. Yao Jiang,Yipeng Li,Qingpeng Shen,Xiaolong Yuan,Fei Gao,Bin Ma. 2025

[9]A putative maize zinc-finger protein gene, ZmAN13, participates in abiotic stress response. Xuan, Ning,Jin, Ying,Zhang, Hongwei,Wang, Guoying,Xie, Yuanhong,Liu, Yunjun,Wang, Guoying.

[10]Genetic diversity analysis of abiotic stress response gene TaSnRK2.7-A in common wheat. Zhang, Hongying,Mao, Xinguo,Zhang, Jianan,Chang, Xiaoping,Jing, Ruilian,Zhang, Hongying,Wang, Chengshe.

[11]Overexpression of a Wheat CCaMK Gene Reduces ABA Sensitivity of Arabidopsis thaliana During Seed Germination and Seedling Growth. Yang, Cui,Kang, Zhensheng,Yang, Cui,Kang, Zhensheng,Yang, Cui,Li, Aili,Zhang, Zenglin,Zhu, Yuanfang,Tan, Xiaomei,Guo, Hanzi,Zhang, Xueyong,Mao, Long,Zhao, Yongliang,Geng, Shuaifeng.

[12]Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress. Xin Zhou,Ling Su,Rui Tang,Yuxuan Dong,Fei Wang,Rong Li,Quanliang Xie,Xianliang Zhang,Guanghui Xiao,Hongbin Li. 2023

[13]Functional Identification Reveals That TaTGA16-2D Promotes Drought and Heat Tolerance. Jingna Ru,Jiamin Hao,Xiaoqian Ji,Bingqing Hao,Jiale Yang,Hongtao Wang,Baoquan Quan,Pengyan Guo,Jiping Zhao,Chao Wang,Huawei Shi,Zhaoshi Xu. 2025

[14]Genome-wide identification, expression analysis, and response to abiotic stress of the phosphate transporter gene family in cucumber (Cucumis sativus L.). Kunhao Xie,Mintao Sun,Xiaoqin Wang,Xiaoya Zhou,Qinghua Di,Yang Li,Guoxiu Wu,Yansu Li,Chaoxing He,Shengli Li. 2025

[15]Protein Post-Translational Modifications in Plant Abiotic Stress Responses. Li, Gengmi,Feng, Baohua,Zhu, Qian-Hao,Jiang, Kaifeng,Zhang, Tao. 2025

[16]Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.). Kong, W. D.,Zhu, Y. G.,Liang, Y. C.,Zhang, J.,Smith, F. A.,Yang, A.. 2007

[17]Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.. Chao, Yuehui,Kang, Junmei,Yang, Qingchuan,Wu, Mingsheng,Li, Yan,Chao, Yuehui,Wang, Pingqing,Long, Ruicai,Qin, Zhihui,Sun, Yan. 2009

[18]Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2. Teng, K.,Guo, W. E.,Yuan, J. B.,Chao, Y. H.,Han, L. B.,Xiao, G. Z.,Li, J.. 2016

[19]A novel statistical method for assessing SSR variation in autotetraploid alfalfa (Medicago sativa L.). Liu, Zhi-Peng,Liu, Gong-She,Yang, Qing-Chuan.

[20]Cloning and function analysis of an alfalfa (Medicago sativa L.) zinc finger protein promoter MsZPP. Yang, Qingchuan,Kang, Junmei,Zhang, Tiejun,Sun, Yan,Gruber, Margaret Yvonne,Fang, Feng.

作者其他论文 更多>>