数字农科院2.0

Dihydromyricetin ameliorates lipopolysaccharide‒induced hepatic injury in chickens by activating the Nrf2/Keap1 pathway and regulating mitochondrial dynamics

文献类型： 外文期刊

作者： Liang Yuan;Xinru Jiang;Yani Ren;Bingke Ma;Zhenghua Ji;Shibo Wang;Beili Hao;Changwen Li;Rui Li;Fangping Liu

作者机构：

关键词： Dihydromyricetin;Nrf2/Keap1 pathway, Mitochondrial dynamics, Oxidative stress, Hepatic injury

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2025 年 104 卷 5 期

页码：

收录情况： SCIE（2025版）

摘要： Dihydromyricetin (DHM) is a flavonoid found in vine tea that exhibits various pharmacological characteristics, including antibacterial, antiapoptotic, and antioxidant effects. Our previous study revealed that DHM can alleviate chicken hepatic injury, but the underlying mechanism has not been elucidated. To further investigate the protective mechanism of DHM, this study firstly predicted by network pharmacology that the potential regulatory pathways of DHM on hepatic injury. Subsequently, the experimental models were replicated in vivo and in vitro using Hy‒Line white broiler chickens and chicken primary hepatocytes treated with DHM and with/ without LPS. Network pharmacology results showed that the effect of DHM on hepatic injury might be related to oxidative stress and mitochondrial function. Further experiments showed that DHM significantly reduced LPS‒elicited serum ALT and AST activities, promoted antioxidant enzyme activities and scavenged ROS in chicken liver or primary hepatocytes. Molecular docking studies showed that DHM could directly bind to Nrf2 and Keap1. Furthermore, DHM treatment regulated the expression of Nrf2 and Keap1, thereby upregulating the downstream expression of antioxidant factors, including HO‒1 and NQO1, in vivo and in vitro. Moreover, DHM modulated the expression of mitochondrial dynamics related factors, including Mfn1/2, Opa1, Drp1, and Fis1, meanwhile, DHM ameliorated mitochondrial structural damage and increased the MMP. Overall, these results suggested that DHM activated the Nrf2/Keap1 pathway and regulated the balance between mitochondrial fusion and fission, ultimately alleviating chicken hepatic injury induced by LPS.

分类号：

  • 相关文献

[1]Optimization Of Dynamic Microwave-Assisted Extraction O.f Dihydromyricetin From Ampelopsis G rossedentata Using Response Surface Methodology. Li, W, Chen, YZ, Xu, X, Zheng, C, Zhao, J. 2018

[2]Dihydromyricetin alleviates Escherichia coli lipopolysaccharide-induced hepatic injury in chickens by inhibiting the NLRP3 inflammasome. Chenxi Shi,Jiaqi Wang,Ruichen Zhang,Muhammad Ishfaq,Ying Li,Ruihui Zhang,Chuanbiao Si,Rui Li,Changwen Li,Fangping Liu. 2022

[3]Dihydromyricetin Suppresses Lipopolysaccharide-Induced Intestinal Injury Through Reducing Reactive Oxygen Species Generation and NOD-Like Receptor Pyrin Domain Containing 3 Inflammasome Activation. Chang, Yicong,Jiang, Xinru,Ji, Zhenghua,Gong, Yingchao,Fan, Xianan,Hao, Beili,Yuan, Liang,Muhammad, Ishfaq,Li, Rui,Li, Changwen,Liu, Fangping. 2024

[4]Development of a reliable and convenient lateral flow immunoassay for dihydromyricetin detection in Ampelopsis grossedentata samples. Cui, Xueyan,Cao, Zhen,Yu, Hailong,Li, Hui,Ying, Ying,Luo, Ting,Wang, Jing. 2025

作者其他论文 更多>>