数字农科院2.0

Haplotype-resolved assembly of a pig genome using single-sperm sequencing

文献类型： 外文期刊

作者： Yongchao Niu;Xinhao Fan;Yalan Yang;Jiang Li;Jinmin Lian;Liu Wang;Yongjin Zhang;Yijie Tang;Zhonglin Tang

作者机构：

关键词： （1-1-1）

期刊名称： Communications Biology

ISSN： 2399-3642

年卷期： 2024 年 7 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Single gamete cell sequencing together with long-read sequencing can reliably produce chromosome-level phased genomes. In this study, we employed PacBio HiFi and Hi-C sequencing on a male Landrace pig, coupled with single-sperm sequencing of its 102 sperm cells. A haplotype assembly method was developed based on long-read sequencing and sperm-phased markers. The chromosome-level phased assembly showed higher phasing accuracy than methods that rely only on HiFi reads. The use of single-sperm sequencing data enabled the construction of a genetic map, successfully mapping the sperm motility trait to a specific region on chromosome 1 (105.40–110.70 Mb). Furthermore, with the assistance of Y chromosome-bearing sperm data, 26.16 Mb Y chromosome sequences were assembled. We report a reliable approach for assembling chromosome-level phased genomes and reveal the potential of sperm population in basic biology research and sperm phenotype research.

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