数字农科院2.0

Transcriptome analysis and autophagy investigation of lovo cells stimulated with exosomes derived from t. Asiatica adult worms

文献类型： 外文期刊

作者： Liang Panhong;Li Yanping;Mao Li;Liu Tingli;Zhang Shaohua;Ehsan Muhammad;Wang Liqun;Guo Aimin;Chen Guoliang;Luo Xuenong

作者机构：

关键词： Autophagy; Exosome; Interaction; RNA-seq; Taenia asiatica

期刊名称： Microorganisms

ISSN： 2076-2607

年卷期： 2021 年 9 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： Taenia asiatica is a zoonotic parasite found in the human intestine and pig liver that evolved various strategies to survive the host’s defenses. Exosomes are membranous vesicles released by cells and are an important vehicle in parasite-host interactions. However, no literature exists on the specific infection mechanisms of T. asiatica against the host defense response, and further research is required to understand the parasite-host interaction. In this study, we investigated the host’s differentially expressed genes (DEGs) while stimulating them with exosomes derived from the T. asiatica adult worm (Tas-exo) on LoVo by RNA-seq analysis. Our results identified 348 genes as being significantly differentially expressed for the Tas-exo group when comparing with that of the NC group. Some of these genes are related to modulation of cell proliferation and cell autophagy. Surprisingly, autophagy and cell proliferation have crucial roles in the defense against parasites; accordingly, we detected cell proliferation and autophagy in LoVo cells by CCK8, immunofluorescence, and Western blotting, demonstrating that Tas-exo could inhibit LoVo cell proliferation and autophagy via AMPK pathway. When P62 and p-mTOR/mTOR expression were significantly increased, BeclinI and pAMPK/AMPK were significantly decreased. These results expand our understanding of parasite-host interactions mediated by exosomes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

分类号：

  • 相关文献

[1]Exosomes promote caprine parainfluenza virus type 3 infection by inhibiting autophagy. Li, Huixia,Zhang, Wenwen,Sun, Min,Li, Jizong,Luo, Xuenong,Wang, Liqun,Hao, Fei,Mao, Li,Mao, Li,Li, Wenliang,Liu, Maojun,Zhang, Shaohua,Yang, Leilei,Liang, Panhong,Li, Wenliang,Cai, Xuepeng,Liu, Maojun,Luo, Xuenong. 2020

[2]甘蓝型油菜角果数突变体基因的定位及候选基因分析. 赵改会,,李书宇,, 詹杰鹏,,李晏斌,,师家勤,,王新发,,王汉中. 2021

[3]基于高通量测序的水貂胚胎滞育期和激活期卵巢转录组分析. 韩玉萍,赵向远,范冰峰,刘理想,邵静,许保增. 2021

[4]Comparative Analysis Of Mirnas In Exosomes Released By Sheeppox Virus-Infected Ovine Testicular Cells. He, GT, Wu, JE, Kong, HL, Zhang, YE, Li, YT, Cai, MT, Shaduhan, G, Yan, YT, Zheng, YD, Ding, JT. 2019

[5]Different Diets Change The Expression Of Bovine Serum Extracellular Vesicle-Mirnas. Quan, SY, Nan, XM, Wang, K, Jiang, LS, Yao, JH, Xiong, BH. 2019

[6]Bioinformatics Analysis Of Aberrantly Expressed Exosomal Lncrnas In Oral Squamous Cell Carcinoma (Cal-27 Vs. Oral Epithelial) Cells. Jin, JJ, Huang, ZX, Lu, XY, Wu, SR, Jia, ME, Li, X, Li, ZY, He, XY. 2020

[7]Echinococcus multilocularis infection induces UBE2N suppression via exosomal emu-miR-4989. Cai, Mengting,Ding, Juntao,Li, Yating,He, Guitian,Yang, Jing,Liu, Tingli,Guo, Xiaola,Yang, Xing,Wang, Xiaoqiang,Cho, William C.,Harandi, Majid Fasihi,Zheng, Yadong. 2021

[8]Bioavailability Of Tea Catechins And I.ts Improvement. Cai, Zhuo-Yu,Li, Xu-Min,Xiang, Li-Ping,Shi, Yun-Long,Liang, Jin-Pei,Shi, Meng,Ye, Jian-Hui,Zheng, Xin-Qiang,Yang, Rui,Wang, Kai-Rong,Liang, Yue-Rong,Lu, Jian-Liang. 2018

[9]Overexpression Of The Peat1 Elicitor G.ene From Alternaria Tenuissima I mproves Drought Tolerance In Rice Plants Via Interaction With A Myo-Inositol Oxygenase. Shi, Fachao,Yang, Xiufeng,Zhang, Yi,Dong, Yijie,Qiu, Dewen,Shi, Fachao. 2017

[10]Golden Promise Barley (Hordeum Vulgare) I.s A Suitable Candidate M odel Host For Investigation Interaction With Heterodera Avenae. Peng Huan,Huang Wen-kun,Cui Jiang-kuan,Luo Shu-jie,Liu Jing,Peng De-liang,Kong Ling-an,Qiao Fen,Luo Shu-jie,Jian Heng. 2017

[11]Overlooked Side Effects Of Organic Farming Inputs Attract Soil Insect Crop Pests. Nyamwasa, Innocent,Nyamwasa, Innocent,Yin, Jiao,Li, Ertao,Nyamwasa, Innocent,Li, Xiaofeng,Liu, Jie,Zhang, Shuai,Li, Kebin,Sun, Xiulian. 2020

[12]Transition challenge to organic agriculture: A course for advancing belowground insect pest management. Li, Jin-qiao,Li, Kebin,Li, Xiaofeng,Qin, Jinhui,Sun, Xiulian,Nyamwasa, Innocent,Nyamwasa, Innocent,Zhang, Shuai,Nyamwasa, Innocent,Yin, Jiao. 2020

[13]The Interactions Between Zno Nanoparticles (.Nps) And Alpha-Linolenic Acid ( Lna) Complexed To Bsa Did Not Influence The Toxicity Of Zno Nps On Hepg2 Cells. Zhou, YW, Fang, X, Gong, Y, Xiao, AP, Xie, YX, Liu, LL, Cao, Y. 2017

[14]A Pilot Study On Interaction B.etween Donkey Tetherin And E iav Stains With Different Virulent And Replication Characteristics. Yao, QC, Ma, J, Wang, XF, Guo, MM, Li, YF, Wang, XJ. 2017

[15]Nine Facultative Endosymbionts In Aphids. A. Review. Chen, Julian,Wang, Zhenying,Hatt, Severin,Hatt, Severin,Guo, Jianqing,Guo, Jianqing,Hatt, Severin,He, Kanglai,Francis, Frederic,Guo, Jianqing. 2017

[16]Interactions Between Selenite And Different F.orms Of Antimony And T heir Effects On Root Morphology Of Paddy Rice. Wu, QH, Feng, RW, Guo, JK, Wang, RG, Xu, YM, Fan, ZL, Mo, LY. 2017

[17]Molecular Structure-Affinity Relationship Of Flavonoids I.n Lotus Leaf (Nelumbo N ucifera Gaertn.) On Binding To Human Serum Albumin And Bovine Serum Albumin By Spectroscopic Method. Tang, XS, Tang, P, Liu, LL. 2017

[18]Intramuscular Preadipocytes Impede Differentiation And P.romote Lipid Deposition Of M uscle Satellite Cells In Chickens. Guo, LP, Cui, HX, Zhao, GP, Liu, RR, Li, QH, Zheng, MQ, Guo, YM, Wen, J. 2018

[19]The Effects Of Baicalein Or B.aicalin On The Colloidal S tability Of Zno Nanoparticles (Nps) And Toxicity Of Nps To Caco-2 Cells. Li, YN, Zhang, C, Liu, LL, Gong, Y, Xie, YX, Cao, Y. 2018

[20]Interaction Between Wheat Gliadin And Quercetin Under Different Ph Conditions Analyzed By Multi-Spectroscopy Methods. Wang, QM, Tang, YW, Yang, YX, Zhao, JC, Zhang, YH, Li, L, Wang, Q, Ming, J. 2020

作者其他论文 更多>>