数字农科院2.0

Rinderine suppresses African swine fever virus replication in porcine alveolar macrophages by dual targeting of membrane-associated profilin 1 and phosphatidylethanolamine

文献类型： 外文期刊

作者： Di Liu;Mengxiang Cao;Yijing Wang;Yan Yan;Meilin Li;Huanjie Zhai;Tao Wang;Jing Lan;Yongfeng Li;Feng Liao;Yuan Sun;Lian Feng Li;Hua Ji Qiu

作者机构：

关键词： African swine fever virus;antiviral activities;phosphatidylethanolamine;profilin 1;rinderine

期刊名称： Emerging Microbes and Infections

ISSN： 2222-1751

年卷期： 2025 年 14 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： African swine fever (ASF) is a highly contagious disease caused by African swine fever virus (ASFV), posing a serious threat to the global pig industry. Natural small molecules have been reported to exhibit anti-ASFV potential. This study utilized the previously established high-throughput platform for screening ASFV inhibitors to identify rinderine (RIN), a pyrrolizidine alkaloid that not only significantly inhibits ASFV replication but also maintains sustained anti-ASFV activity at 24, 48, and 72 hours postinfection. Moreover, RIN mainly suppresses the early stages of ASFV replication, particularly by inhibiting ASFV attachment to primary porcine alveolar macrophages (PAMs). Mechanistically, mass spectrometry-based cellular thermal shift assay, further experimental validation and molecular docking analysis indicate that profilin 1 (PFN1) is a key target protein mediating the inhibitory effects of RIN on ASFV attachment to PAMs. Structural prediction analysis, co-immunoprecipitation assay, and confocal microscopy demonstrate that RIN disrupts the interaction between PFN1 and actin, thereby decreasing the actin remodelling. Notably, untargeted metabolomics profiling and further experiments reveal that RIN reduces the phosphatidylethanolamine level derived from the glycerophospholipid metabolism pathway and decreases the membrane permeability of PAMs, contributing to its anti-ASFV activity. In conclusion, integrative proteomics and untargeted metabolomics analyses demonstrate that RIN exerts the antiviral effects against ASFV primarily by targeting proteins and metabolites associated with cell membrane formation and function, providing a novel insight into the prevention and control of ASF.

分类号：

  • 相关文献

[1]Profiling and relative quantification of phosphatidylethanolamine based on acetone stable isotope derivatization. Wang, Xiang,Wei, Fang,Xu, Ji-qu,Lv, Xin,Dong, Xu-yan,Huang, Feng-hong,Chen, Hong,Wang, Xiang,Wei, Fang,Xu, Ji-qu,Lv, Xin,Dong, Xu-yan,Huang, Feng-hong,Chen, Hong,Han, Xianlin,Han, Xianlin,Quek, Siew-young.

[2]Molecular cloning and functional characterization of eleven subtypes of interferon-alpha in Amur tigers (Panthera tigris altaica). Zhao, Hongjing,Ma, Jian,Wang, Yu,Liu, Juanjuan,Shao, Yizhi,Li, Jinglun,Jiang, Guangshun,Xing, Mingwei,Ma, Jian.

[3]RNA interference screening of interferon-stimulated genes with antiviral activities against classical swine fever virus using a reporter virus. Wang, Xiao,Li, Yongfeng,Li, Lian-Feng,Shen, Liang,Zhang, Lingkai,Yu, Jiahui,Luo, Yuzi,Sun, Yuan,Li, Su,Qiu, Hua-Ji.

[4]Inhibition of virus replication and induction of human tetherin gene expression by equine IFN-alpha 1. Hu, Zhe,Wu, Xingliang,Ge, Jinying,Wang, Xiaojun.

[5]Replication And Virulence In Pigs O.f The First African S wine Fever Virus Isolated In China. Wang, Xijun,Wu, Xinzhou,Bu, Zhigao,Chen, Weiye,Liu, Xing,Wang, Lulu,Bu, Zhigao,Liu, Renqiang,Wang, Jingfei,Zhang, Jiwen,Zhang, Xianfeng,Zhao, Dongming,Li, Fang,Zhang, Jiaoer,Guan, Yuntao,He, Xijun. 2019

[6]Pre-Clinical Evaluation of a Real-Time PCR Assay on a Portable Instrument as a Possible Field Diagnostic Tool: Experiences from the Testing of Clinical Samples for African and Classical Swine Fever Viruses. Liu, L.,Stahl, K.,Belak, S.,Liu, L.,Stahl, K.,Belak, S.,Liu, L.,Luo, Y.,Qiu, H. -J.,Luo, Y.,Qiu, H. -J.,Accensi, F.,Ganges, L.,Rodriguez, F.,Accensi, F.,Rodriguez, F.,Shan, H.,Belak, S..

[7]A comparative analysis on the synonymous codon usage pattern in viral functional genes and their translational initiation region of ASFV. Zhou, Jian-hua,Gao, Zong-liang,Sun, Dong-jie,Ding, Yao-zhong,Zhang, Jie,Liu, Yong-sheng,Stipkovits, Laszlo,Szathmary, Susan,Pejsak, Zygmunt. 2013

[8]Recombinant Newcastle disease virus expressing African swine fever virus protein 72 is safe and immunogenic in mice. Chen, Xinxin,Yang, Jifei,Ji, Yanhong,Liu, Bin,Li, Xiaoyang,Yin, Hong,Zhu, Qiyun,Yin, Hong,Okoth, Edward. 2016

[9]Genome Sequences Derived From Pig A.nd Dried Blood Pig F eed Samples Provide Important Insights Into The Transmission Of African Swine Fever Virus In China In 2018. Wen, XX, He, XJ, Zhang, X, Zhang, XF, Liu, LL, Guan, YT, Zhang, Y, Bu, ZG. 2019

[10]Pre-Clinical Evaluation Of A Real-Time P.cr Assay On A P ortable Instrument As A Possible Field Diagnostic Tool: Experiences From The Testing Of Clinical Samples For African And Classical Swine. Liu, L, Luo, Y, Accensi, F, Ganges, L, Rodriguez, F, Shan, H, Stahl, K, Qiu, HJ, Belak, S. 2017

[11]Roles Of African Swine Fever V.irus Structural Proteins In V iral Infection. Jia, N, Ou, YW, Pejsak, Z, Zhang, YG, Zhang, J. 2017

[12]CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis. Xin Chen,Jun Zheng,Chuanxia Liu,Tingting Li,Xiao Wang,Xuewen Li,Miaofei Bao,Jiangnan Li,Li Huang,Zhaoxia Zhang,Zhigao Bu,Changjiang Weng. 2023

[13]Recombinant porcine interferon cocktail delays the onset and lessens the severity of African swine fever. Pengtao Jiao,Shuchao Wang,Wenhui Fan,He Zhang,Hongyan Yin,Yingli Shang,Hongfei Zhu,Wenjun Liu,Rongliang Hu,Lei Sun. 2023

[14]The African swine fever virus I10L protein inhibits the NF-κB signaling pathway by targeting IKKβ. Xing Chen,Lian Feng Li,Zhong Yuan Yang,Meilin Li,Shuai Fan,Lan Fang Shi,Zi Yu Ren,Xue Jing Cao,Yuhang Zhang,Shichong Han,Bo Wan,Hua Ji Qiu,Gaiping Zhang,Wen Rui He. 2023

[15]Development of a TaqMan-Probe-Based Multiplex Real-Time PCR for the Simultaneous Detection of African Swine Fever Virus, Porcine Circovirus 2, and Pseudorabies Virus in East China from 2020 to 2022. Huaicheng Liu,Jianwen Zou,Rongchao Liu,Jing Chen,Xiaohan Li,Haixue Zheng,Long Li,Bin Zhou. 2023

[16]Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches. Gao Zhan,Shao Jun-Jun,Zhang Guang-Lei,Ge Su-Dan,Chang Yan-Yan,Xiao Lei,Chang Hui-Yun. 2021

[17]Mechanism of interaction between virus and host is inferred from the changes of gene expression in macrophages infected with African swine fever virus CN/GS/2018 strain. Bo Yang,Chaochao Shen,Dajun Zhang,Ting Zhang,Xijuan Shi,Jinke Yang,Yu Hao,Dengshuai Zhao,Huimei Cui,Xingguo Yuan,Xuehui Chen,Keshan Zhang,Haixue Zheng,Xiangtao Liu. 2021

[18]Development and in vivo evaluation of MGF100-1R deletion mutant in an African swine fever virus Chinese strain. Yingnan Liu,Yao Li,Zhenhua Xie,Qingying Ao,Dongdong Di,Wanqi Yu,Lu Lv,Qiuping Zhong,Yingying Song,Xinxin Liao,Qingqing Song,Heng Wang,Hongjun Chen. 2021

[19]Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI. Guanglei Zhang,Wei Liu,Zhan Gao,Yanyan Chang,Sicheng Yang,Qian Peng,Sudan Ge,Bijing Kang,Junjun Shao,Huiyun Chang. 2022

[20]Emergence and prevalence of naturally occurring lower virulent African swine fever viruses in domestic pigs in China in 2020. Encheng Sun,Zhenjiang Zhang,Zilong Wang,Xijun He,Xianfeng Zhang,Lulu Wang,Wenqing Wang,Lianyu Huang,Fei Xi,Haoyue Huangfu,Ghebremedhin Tsegay,Hong Huo,Jianhong Sun,Zhijun Tian,Wei Xia,Xuewu Yu,Fang Li,Renqiang Liu,Yuntao Guan,Dongming Zhao,Zhigao Bu. 2021

作者其他论文 更多>>