数字农科院2.0

Molecular characterization and immune protective efficacy of 3 Eimeria tenella antigens

文献类型： 外文期刊

作者： Zhang, Lei;Song, Zhixuan;Li, Peiyao;Song, Xingju;Tang, Xinming;Hu, Dandan

作者机构：

关键词： Eimeria tenella;recombinant proteins;protective efficacy;IFN-g

期刊名称： POULTRY SCIENCE

ISSN： 0032-5791

年卷期： 2024 年 103 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Avian coccidiosis caused by Eimeria is a serious parasitic disease that poses a threat to the poultry industry. Currently, prevention and treatment mainly rely on the administration of anticoccidials and live oocyst vaccines. However, the prevalence of drug resistance and the inherent limitations of live vaccines have driven the development of novel vaccines. In this study, the surface protein (Et-SAG14), a previously annotated rhoptry protein (Eten5-B), and a gametocyte phosphoglucomutase (Et-PGM1) were characterized and the vaccine potential of the recombinant proteins were evaluated. Et-SAG14 was dispersed in the form of particles in the sporozoite and merozoite stages, whereas Et-PGM1 was distributed in the apical part of the sporozoite and merozoite stages. The previously annotated rhoptry Eten5-B was found not to be located in the rhoptry but distributed in the cytoplasm of sporozoites and merozoites. Immunization with rEten5-B significantly elevated host interferon gamma (IFN-gamma) and interleukin 10 (IL-10) transcript levels and exhibited moderate anticoccidial effects with an anticoccidial index (ACI) of 161. Unexpectedly, both recombinant Et-SAG14 and Et-PGM1 immunization significantly reduced host IFN-gamma and IL-10 transcription levels, and did not show protection against E. tenella challenge (ACI < 80). These results suggest that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.

分类号：

  • 相关文献

[1]Construction of recombinant proteins for reprogramming of endangered Luxi cattle fibroblast cells. Hu, P. F.,Guan, W. J.,Li, X. C.,Ma, Y. H..

[2]Molecular cloning and characterization of three isoprenyl diphosphate synthase genes from alfalfa. Sun, Yan,Zhou, He,Zhang, Ze,Kang, Junmei,Zhang, Tiejun,Yang, Qingchuan.

[3]Molecular cloning and characterization of two alanine aminotransferase genes in the white-backed planthopper Sogatella furcifera. Fan, Jin-Mei,Wan, Pin-Jun,Li, Na,Mu, Li-Li,Li, Guo-Qing,Fu, Qiang.

[4]High-yield production of acidic pectin lyase PNLZJ5B for juice processing. Liu C., Qin X., Liu B., Xu X., Deng A., Zhang Y., Zhang Z., Zhang W.. 2022

[5]Harnessing Transient Expression Systems with Plant Viral Vectors for the Production of Biopharmaceuticals in Nicotiana benthamiana. Sayed Abdul Akher,Kevin Yueju Wang,Kylie Hall,Oluwaseyi Setonji Hunpatin,Muhammad Shan,Zenglin Zhang,Yongfeng Guo. 2025

[6]Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Zhao, Yu,Li, Zhong-Yuan,Chen, Jia,Liu, Shan-Shan,Zhu, Xing-Quan,Zhou, Dong-Hui,Zhao, Yu,Sun, Xiao-Lin,Li, Zhong-Yuan,Liu, Shan-Shan,Zhu, Xing-Quan.

[7]Deletion of the BAC sequences from recombinant meq-null Marek's disease (MD) virus increases immunosuppression while maintaining protective efficacy against MD. Su, S.,Cui, N.,Sun, P.,Cui, Z.,Li, J.,Li, H.,Li, Y..

[8]Construction and immune effect of Haemophilus parasuis DNA vaccine encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in mice. Bei, Weicheng,Fu, Shulin,Ou, Jiwen,Tan, Chen,Chen, Huanchun,Bei, Weicheng,Zhang, Minmin,Liu, Jinlin. 2012

[9]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[10]Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[11]Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation. Wen, Feng,Ma, Ji-Hong,Yu, Hai,Yang, Fu-Ru,Huang, Meng,Zhou, Yan-Jun,Li, Ze-Jun,Tong, Guang-Zhi.

[12]Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Li, Huixin,Wang, Yulong,Han, Zongxi,Wang, Yu,Liang, Shulin,Jiang, Lu,Kong, Xiangang,Liu, Shengwang,Wang, Yulong,Hu, Yonghao.

[13]Protective Efficacy of an H5N1 Inactivated Vaccine Against Challenge with Lethal H5N1, H5N2, H5N6, and H5N8 Influenza Viruses in Chickens. Tian, Guobin,Chen, Hualan.

[14]Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Zhang, Yanping,Gao, Yulong,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[15]Evaluation of two strains of Mareles disease virus serotype 1 for the development of recombinant vaccines against very virulent infectious bursal disease virus. Li, Kai,Liu, Yongzhen,Liu, Changjun,Gao, Li,Gao, Yulong,Zhang, Yanping,Cui, Hongyu,Qi, Xiaole,Zhong, Li,Wang, Xiaomei.

[16]Cross-protective efficacy of engineering serotype A foot-and-mouth disease virus vaccine against the two pandemic strains in swine. Zheng, Haixue,Lian, Kaiqi,Yang, Fan,Jin, Ye,Zhu, Zixiang,Guo, Jianhong,Cao, Weijun,Liu, Huanan,He, Jijun,Zhang, Keshan,Li, Dan,Liu, Xiangtao.

[17]Protective efficacy of a novel recombinant Marek's disease virus vector vaccine against infectious bursal disease in chickens with or without maternal antibodies. Li, Kai,Liu, Yongzhen,Zhang, Yanping,Gao, Li,Liu, Changjun,Cui, Hongyu,Qi, Xiaole,Gao, Yulong,Zhong, Li,Wang, Xiaomei.

[18]Protective Efficacy of the Inactivated H5N1 Influenza Vaccine Re-6 Against Different Clades of H5N1 Viruses Isolated in China and the Democratic People's Republic of Korea. Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Zeng, Xianying,Deng, Guohua,Liu, Liling,Li, Yanbing,Shi, Jianzhong,Chen, Pucheng,Feng, Huapeng,Tian, Guobin,Chen, Hualan,Liu, Jingli,Guo, Xingfu,Mao, Shenggang,Yang, Fan,Chen, Zhiyu.

[19]Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain. Li, Jiangnan,Song, Jie,Zhou, Shijun,Li, Shuai,Liu, Jia,Li, Tingting,Zhang, Zhaoxia,Zhang, Xianfeng,He, Xijun,Chen, Weiye,Zheng, Jun,Zhao, Dongming,Bu, Zhigao,Huang, Li,Weng, Changjiang. 2023

[20]Protection Evaluation of a Five-Gene-Deleted African Swine Fever Virus Vaccine Candidate Against Homologous Challenge. Xie Z.,Liu Y.,Di D.,Liu J.,Gong L.,Chen Z.,Li Y.,Yu W.,Lv L.,Zhong Q.,Song Y.,Liao X.,Song Q.,Wang H.,Chen H.. 2022

作者其他论文 更多>>