数字农科院2.0

Genome-wide association studies identified OsTMF as a gene regulating rice seed germination under salt stress

文献类型: 外文期刊

作者: Lifeng Liu;Yanling Ma;Heng Zhao;Lin Guo;Yan Guo;Chun Ming Liu

作者机构:

关键词: GWAS;OsTMF;rice;salt stress;seed germination

期刊名称: Frontiers in Plant Science

ISSN: 1664-462X

年卷期: 2024 年 15 卷

页码:

收录情况: SCIE(2024版)

摘要: Introduction: Salt tolerance during seed germination is an important trait for direct seeding and low-cost rice production. Nevertheless, it is still not clear how seed germination under salt stress is regulated genetically. Methods: In this study, genome-wide association studies (GWAS) were performed to decipher the genetic basis of seed germination under salt stress using 541 rice varieties collected worldwide. Results and discussion: Three quantitative trait loci (QTLs) were identified including qGRG3-1 on chromosome 3, qGRG3-2 on chromosome 5, and qGRG4 on chromosome 4. Assessment of candidate genes in these loci for their responses to salt stress identified a TATA modulatory factor (OsTMF) in qGRG3-2. The expression of OsTMF was up-regulated in both roots and shoots after exposure to salt stress, and OsTMF knockout mutants exhibited delayed seed germination under salt stress. Haplotype analysis showed that rice varieties carrying OsTMF-Hap2 displayed elevated salt tolerance during seed germination. These results provide important knowledge and resources to improve rice seed germination under salt stress in the future.

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