数字农科院2.0

Elimination of Cefquinome Sulfate Residue in Cow’s Milk after Intrauterine Infusion

文献类型： 外文期刊

作者： Chunshuang Liu;Mingyue Han;Honglei Wang;Xiaojie Chen;Yaoxin Tang;Daokang Zhang;Xiubo Li;Yiming Liu

作者机构：

关键词： cefquinome sulfate intrauterine infusion;cow;milk;residue;UPLC-MS/MS

期刊名称： Metabolites

ISSN： 2218-1989

年卷期： 2023 年 13 卷 4 期

页码：

收录情况： SCIE（2023版）

摘要： As set in the maximum residue limit regulations of the European Commission, this study aimed to obtain the residual parameters in milk with optimized UPLC-MS/MS conditions and to determine the conclusive drug withdrawal period to ensure food safety. In this research, an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to study cefquinome sulfate’s residue elimination in milk and to calculate cefquinome’s withdrawal period. Twelve healthy cows free of endometritis were selected for the experiment. Before using the drug, the vaginal orifice and perineum of each cow was disinfected. One dose of intrauterine perfusion was used for each cow, followed by an additional dose after 72 h. Before administration and 12 h, 18 h, 24 h, 36 h, 42 h, 48 h, 60 h, 66 h, 72 h, 84 h, 90 h, and 96 h after the last dose, milk (10 mL) was gathered from each cow’s teat and pooled. For the measurement of cefquinome concentrations in milk, UPLC-MS/MS was performed. A calibration curve was generated using linear regression as follows: Y = 250.86X − 102.29, with a correlation coefficient of 0.9996; the limits of detection and the limits of quantitation were 0.1 μg·kg−1 and 0.2 μg·kg−1, respectively. The average recovery of cefquinome was 88.60 ± 16.33% at 0.2 μg·kg−1, 100.95 ± 2.54% at 10 μg·kg−1, and 97.29 ± 1.77% at 50 μg·kg−1. For 5 consecutive days at the three spiking levels, the intra and inter-day relative standard deviations (RSD) were 1.28%–13.73% and 1.81%–18.44%, respectively; the residual amount of cefquinome was less than the maximum residue limit of 20 μg·kg−1, 36 h after administration; and the residual amount was less than the limit of detection (0.1 μg·kg−1) 48 h after administration. The withdrawal time of cefquinome in cow’s milk was 39.8 h, as calculated using WTM1.4 software. In terms of clinical practical use, the withdrawal period of milk was temporarily set at 48 h after the administration of the cefquinome sulfate uterus injection to cows, in accordance with the recommended dose and course.

分类号：

  • 相关文献

[1]Analysis of 22 Elements in Milk, Feed, and Water of Dairy Cow, Goat, and Buffalo from Different Regions of China. Zhou, Xuewei,Qu, Xueyin,Zhao, Shengguo,Wang, Jiaqi,Li, Songli,Zheng, Nan,Zhou, Xuewei,Qu, Xueyin,Zhao, Shengguo,Wang, Jiaqi,Li, Songli,Zheng, Nan.

[2]Accumulation and Depletion of Cadmium in the Blood, Milk, Hair, Feces, and Urine of Cows During and After Treatment. Su, Chuanyou,Zhang, Junmin,Zhao, Qingyu,Su, Chuanyou,Li, Zhentian,Liu, Kaidong,Sun, Youde,Wang, Jianhua.

[3]Comparative Proteomic Analysis of Milk-Derived Extracellular Vesicles from Dairy Cows with Clinical and Subclinical Mastitis. Mengling Wang,Meng Cai,Xiaoyan Zhu,Xuemei Nan,Benhai Xiong,Liang Yang. 2023

[4]Multi-residue determination of 210 drugs in pork by ultra-high-performance liquid chromatography-tandem mass spectrometry. Yin, Zhiqiang,Chai, Tingting,Mu, Pengqian,Xu, Nana,Song, Yue,Wang, Xinlu,Jia, Qi,Qiu, Jing.

[5]Determination of ametoctradin residue in fruits and vegetables by modified quick, easy, cheap, effective, rugged, and safe method using ultra-performance liquid chromatography/tandem mass spectrometry. Liu, Xingang,Dong, Fengshou,Xu, Jun,Li, Shasha,Xu, Hanqing,Zheng, Yongquan. 2015

[6]Simultaneous determination of oxathiapiprolin and two metabolites in fruits, vegetables and cereal using a modified quick, easy, cheap, effective, rugged, and safe method and liquid chromatography coupled to tandem mass spectrometry. Xu, Jun,Dong, Fengshou,Liu, Xingang,Li, Yuanbo,Zheng, Yongquan. 2014

[7]Quantitative determination of pyriproxyfen and its metabolite residues in bee products of China using a modified QuEChERS approach with UPLC-MS/MS. Chi Wu,Xingang Liu,Mingyuan He,Fengshou Dong,Xiaohu Wu,Jun Xu,Yongquan Zheng. 2021

[8]Simultaneous Determination of 5 Peptide Antibiotics in Bovine Milk Samples by Liquid Chromatography-Tandem Mass Spectrometry. Liu Jia-Jia,Jin Fen,She Yong-Xin,Liu Hong-Bin,Shi Xiao-Mei,Wang Miao,Wang Jing,Xu Si-Yuan. 2011

[9]Simultaneous quantification of oxyclozanide and levamisole in milk via ultra-high-performance liquid chromatography tandem mass spectrometry: A validated high-sensitivity approach for veterinary residue surveillance. G. Dai,K. Sun,Y. Zhou,B. Li,Q. Wang,Y. Qiu,X. Peng,X. Zhou,J. Zhang. 2025

[10]QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中5种孕激素. 黄胜广,赵卉,罗婧,王玉方,刘继永,王峰. 2019

[11]人参中28种人参皂苷含量的UPLC-MS/MS同时检测. 赵立春,王玉方,刘继永. 2019

[12]四种除草剂原药有效成分UPLC-MS/MS测定方法研究. 许庆轩,张婷婷,周芹,吴玉梅. 2011

[13]超高效液相色谱-串联质谱法检测棉花和土壤中呋虫胺残留. 徐军,张盈,董丰收,刘新刚,郑永权. 2015

[14]基于广泛靶向代谢组学的不同颜色棉花花瓣中类黄酮成分差异分析. 李秋琳,李燕,陈伟,姚金波,朱守鸿,袁黎,张永山. 2021

[15]甜菜中4种除草剂残留的超高效液相色谱—串联质谱检测方法研究. 王皙玮,刘质静,胡晓航,张福顺,许庆轩,杨晶. 2013

[16]超高效液相色谱串联质谱检测啶菌噁唑等6种农药在我国典型土壤中的残留. 焦斌,徐军,刘新刚,董丰收,吴小虎,郑永权. 2020

[17]QuEChERS结合超高效液相色谱-串联质谱法同时测定梅花鹿鹿茸中18种磺胺. 黄胜广,赵卉,王玉方,金美伶,宋超,王峰. 2019

[18]QuEChERS/UPLC-MS/MS法同时测定梅花鹿鹿茸中7种镇静剂. 黄胜广,王玉方,赵卉,毕融冰,刘炎,王峰. 2020

[19]煮炸鹿茸加工过程中晚期糖基化终产物生成规律及动力学分析. 宫瑞泽,刘松鑫,邵紫君,夏蕴实,张磊,王玉方,孙印石,刘畅. 2020

[20]QuEChERS/UPLC-MS/MS法测定梅花鹿鹿茸中的噻拉嗪及其代谢产物. 黄胜广,王玉方,赵卉,金春爱,毕融冰,王峰. 2020

作者其他论文 更多>>