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Recombinant duck enteritis virus harboring the hemagglutinin genesof influenza virus rapidly induces specifie cellular immunity in ducks

文献类型： 外文期刊

作者： 赵玉博;;麻琦;;焦晨晨;;刘静;;张小雨;;陈普成;;温志远;;姜永萍;;曾显营;;邓国华;;施建忠;;李雁冰;;田国彬;;陈化兰;;柳金雄

关键词： Duck enteritis virus; Avian influenza; Vaccine; Innate immunity; Cellular immunity.

期刊名称： Journal of Virology

ISSN： 0022-538X

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Given that ducks serve as critical reservoirs for avian influenza viruses, achieving high immune coverage in duck flocks is essential for preventing the transmission of avian influenza viruses from wild birds to domestic poultry. Duck enteritis is the most important infectious disease that must be prevented with a live-attenuated vaccine in duck breeding. Therefore, we previously constructed a recombinant duck enteritis virus (DEV), rDEV-dH5/H7, which carries the hemagglutinin (HA) genes of two H5 viruses and an H7 influenza virus. It could induce rapid and complete protection against both lethal DEV and H5 and H7 viruses as early as 7 days post-prime vaccination, although almost no antibodies were detected in ducks at this time. In the present study, we demonstrated that rDEV-dH5/H7 immediately initiated innate immune responses and T-cell proliferation in ducks. The expression levels of IFN-γ and granzyme A were markedly upregulated at 5 to 7 days post-prime vaccination. The percentages of CD3+ and CD3+CD8+ T cells in peripheral blood mononuclear cells (PBMCs) significantly increased from 7 days post-prime vaccination. Moreover, HA-specific CD8+ and CD4+ T cells, as well as those induced by DEV virion were significantly higher than those in control animals from 7 days post-prime vaccination. Together, the quick response of specific T cells and the innate immune response induced by rDEV-dH5/H7 may confer rapid immune protection against lethal influenza virus and DEV.

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