数字农科院2.0

Unveiling side-chain architectures of corn husk hemicellulose to drive enzyme cocktail design for degradation of biomass

文献类型： 外文期刊

作者： Yingjie Guo;Zheng Zhang;Xuezhi Li;Jian Zhao

作者机构：

关键词： Corn husk hemicellulose;One-step enzymatic hydrolysis;Structural characterization

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年 310 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： By analyzing residual hemicellulose after enzymatic hydrolysis of corn husks, we identified previously unreported side-chain architectures in glucuronoarabinoxylan and xyloglucan of corn husk hemicellulose, including α-galactosyl-mannose and α-xylosyl-arabinose linkages, which shield glycosidic bonds from enzymatic cleavage. These discoveries unveil novel structural complexes in hemicellulose and broaden the understanding of hemicellulose recalcitrance beyond existing literature. Further, we designed a tailored enzyme cocktail by heterologously expressing three targeted hemicellulases (GH51 α-L-arabinofuranosidase, GH27 α-D-galactosidase, and GH31 α-D-xylosidase) based on hemicellulose structural information, and synergistic integration of these enzymes with Penicillium oxalicum cellulase via response surface optimization. This enzyme system achieved unprecedented yields of 81.82 % glucose and 54.89 % arabinose through one-step hydrolysis without chemical pretreatment, surpassing previous benchmarks by over 30 %. This study advances the understanding of hemicellulose's intricate structural complexity and enhances insights into multi-enzyme synergies during biomass degradation, offering a pivotal framework for developing pretreatment-free, eco-friendly enzymatic hydrolysis processes and enabling high-value utilization of corn husks.

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