数字农科院2.0

Morphological and molecular identification of Spodoptera frugiperda, an alien lepidopteran pest invaded maize fields in Pakistan

文献类型： 外文期刊

作者： Waqas Wakil;Huiru Jia;Nickolas G. Kavallieratos;Nikoleta Eleftheriadou;Abid Ali;Pasco B. Avery

作者机构：

关键词： Adult;DNA sequencing;Fall armyworm;Larva;Polymorphic loci;Strains

期刊名称： Phytoparasitica

ISSN： 0334-2123

年卷期： 2026 年 54 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： Spodoptera frugiperda, a pest long established in North and South America, was first reported in Africa in 2016 and subsequently detected in several Asian countries, including China and Pakistan. This pest has caused significant losses in maize crops of the Sindh Province (Pakistan), where it was initially reported based on larval morphology. However, the S. frugiperda plant host strains (maize or rice) present in Pakistan have not been verified using molecular techniques. In October 2019, three maize fields in the Okara, Pattoki, and Depalpur regions of Punjab Province, eastern Pakistan, were surveyed. Spodoptera frugiperda individuals initially collected as larvae and later as emerged adults were morphologically identified. Sequence analysis of 60 larvae (20 from each field) targeting the sex-linked Triosephosphate isomerase (Tpi) gene and the mitochondrial Cytochrome c Oxidase Subunit I (COI) gene confirmed that all sampled specimens were S. frugiperda. Further analysis of Tpi gene polymorphisms identified all individuals as the maize strain, with some exhibiting heterozygosity deficiencies at two loci. This study represents the first detection of S. frugiperda in Pakistan using both morphological and molecular techniques, contributing to more accurate identification and enabling timely management interventions.

分类号：

  • 相关文献

[1]Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR. Yan An,Zhou ZhenFeng,Dong Hui,Zhang YaKun,Jin Li,Zhao GuoPing,Xiong Hui,Zhu Lei,He YunGang,Zhao GuoPing,Jin WeiRong,Zhang GuoQing,Li YiXue,Yang Zhong,Zhong Yang,Cao ZhiWei,Jin Li,Wang HongYan,Wang XiaoNing,Yang Zhong,Zhong Yang,Zhao GuoPing,Wang XiaoNing,Dai JianXin,Guo YaJun,Wang Hao,Dai JianXin,Guo YaJun,Wang Hao,Che XiaoYan,Wu Fan,Yuan ZhenGan,Zhang Xi,Cao ZhiWei,Zhou XiaoNong,Zhou JiaHai,Ma ZhiYong,Tong GuangZhi,Ding GuoHui,Li YiXue,Sun Bing,Huang Zhong,Lan Ke,Jin Li,Zhao GuoPing,Ding GuoHui,Li YiXue,Sun Bing,Huang Zhong,Lan Ke,Jin Li,Zhao GuoPing,Ding GuoHui,Li YiXue,Sun Bing,Huang Zhong,Lan Ke,Jin Li,Zhao GuoPing,Ding GuoHui,Li YiXue,Sun Bing,Huang Zhong,Lan Ke,Jin Li,Zhao GuoPing,Ding GuoHui,Li YiXue,Sun Bing,Huang Zhong,Lan Ke,Jin Li,Zhao GuoPing. 2009

[2]Changes of proteome and phosphoproteome trigger embryo-larva transition of honeybee worker (Apis mellifera ligustica). Gala, Alemayehu,Fang, Yu,Woltedji, Dereje,Zhang, Lan,Han, Bin,Feng, Mao,Li, Jianke.

[3]Preliminary observations on the physiology and behavior of Sytretomorpha szaboi Papp. Zhang, Jun,Li, Jiali,Xiong, Jian,Geng, Mingyang,Wu, Ping,Tang, Hong,Li, Xijie,Zhao, Min,Zhao, Yazhou,Guo, Jun. 2024

[4]Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Zhu, S,Wang, HP,Wang, LL,Tang, B,Li, R,Wan, R,Liu, Y,Huang, YH,Zhang, L,Sun, XZ,Li, N.

[5]Proteomic analysis of differentially expressed proteins in the three developmental stages of Trichinella spiralis. Liu, J. Y.,Zhang, N. Z.,Li, W. H.,Li, L.,Yan, H. B.,Qu, Z. G.,Li, T. T.,Cui, J. M.,Yang, Y.,Jia, W. Z.,Fu, B. Q.,Jia, W. Z.,Fu, B. Q..

[6]Progress in Genomic Mating in Domestic Animals. Zhang P., Qiu X., Wang L., Zhao F.. 2022

[7]Sex and Body Colour Affect the Variation in Internal Body Temperature of Oedaleus decorus asiaticus in Natural Habitats in Inner Mongolia, China. Cheng, Yumeng,Li, Hongmei,Liu, Lulu,Wang, Guangjun,Gu, Haojing,Luke, Belinda. 2022

[8]Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer. Hu, Ben-Jin,Xu, Li-Na,Wang, Yue-Qin,Wang, Zhen-Ying,He, Kang-Lai,Ling, Ying-Hui. 2015

[9]SSR marker-based mapping and linkage analysis of Bombyx mori thermotolerance gene. Zhao, Yuan,Wu, Yang-Chun,Zhu, Ya-Hong,Zhao, Yuan,Zhang, Jiang.

[10]Identification of crucial residues of conformational epitopes on VP2 protein of infectious bursal disease virus by phage display. Cui, XL,Nagesha, HS,Holmes, IH.

[11]Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. Liang, Gemei,Li, Haitao,He, Kanglai,Song, Fuping,Feng, Xue,Zhang, Jie,Crickmore, Neil,Huang, Dafang.

[12]Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests. Wang, Guangjun,Zhang, Jie,Song, Fuping,Wu, Jun,Feng, Shuliang,Huang, Dafang.

[13]Quality assessment of Telenomus remus successively reared on Spodoptera litura eggs for 30 generations. Chen, Wanbin,Liu, Huan,Chen, Bin,Chen, Junjie,Wang, Mengqing,Shen, Zhongjian,Li, Yuyan,Mao, Jianjun,Zhang, Lisheng. 2023

[14]Global genomic signature reveals the evolution of fall armyworm in the Eastern hemisphere. Zhang, Lei,Li, Zaiyuan,Peng, Yan,Liang, Xinyue,Wilson, Kenneth,Chipabika, Gilson,Karangwa, Patrick,Uzayisenga, Bellancile,Mensah, Benjamin A.,Kachigamba, Donald L.,Xiao, Yutao. 2023

[15]Management of Spodoptera frugiperda J.E. Smith Using Recycled Virus Inoculum from Larvae Treated with Baculovirus under Field Conditions. Allan Mweke,Ivan Rwomushana,Arthur Okello,Duncan Chacha,Jingfei Guo,Belinda Luke. 2023

[16]Cold storage effects on biological parameters of Telenomus remus, a promising egg parasitoid of Spodoptera frugiperda, reared on Spodoptera litura eggs. Chen, Wanbin,Li, Yuyan,Zhang, Changhua,Jia, Fangzhao,Zhang, Maosen,Wang, Mengqing,Mao, Jianjun,Zhang, Lisheng. 2022

[17]Push-pull plants in wheat intercropping system to manage Spodoptera frugiperda. Liu, Huan,Cheng, Yumeng,Wang, Qian,Liu, Xiaobei,Fu, Yu,Zhang, Yong,Chen, Julian. 2022

[18]Population occurrence of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), in the winter season of China. . 2020

[19]Management of Spodoptera frugiperda JE Smith Using Recycled Virus Inoculum from Larvae Treated with Baculovirus under Field Conditions. Mweke, Allan,Rwomushana, Ivan,Okello, Arthur,Chacha, Duncan,Guo, Jingfei,Luke, Belinda. 2023

[20]Morphological defense of the egg mass of Spodoptera frugiperda (Lepidoptera: Noctuidae) affects parasitic capacity and alters behaviors of egg parasitoid wasps. Hui Dong,Kai hui Zhu,Qian Zhao,Xue ping Bai,Jin cheng Zhou,Li sheng Zhang. 2021

作者其他论文 更多>>