数字农科院2.0

Revealing the Mechanism of Protein Degradation in Postmortem Meat: The Role of Phosphorylation and Ubiquitination

文献类型： 外文期刊

作者： Xinran Zhao;Saisai Wu;Chi Ren;Yuqiang Bai;Chengli Hou;Xin Li;Zhenyu Wang;Dequan Zhang

作者机构：

关键词： AMPK;E3 ubiquitin ligase;meat tenderness;protein posttranslational modifications

期刊名称： Foods

ISSN： 2304-8158

年卷期： 2025 年 14 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： The aim of this study was to investigate the possible effects of phosphorylation and ubiquitination on the degradation of myofibrillar proteins in mutton with different tenderness. The longissimus thoracis lumborum muscles were chosen and divided into tender and tough groups (n = 9), and then stored at 4 °C for 1 h, 12 h, 1 d, 3 d, and 5 d postmortem. Shear force, pH, myofibril fragmentation index, AMPK activity, E3 ubiquitin ligase abundance, protein phosphorylation, and the ubiquitination levels of muscle samples were measured. The results demonstrated that the meat of samples in the tender group had a higher degradation of desmin and a lower phosphorylation level of desmin at 1 d compared with the tough group. The ubiquitination level of desmin, AMPK activity, and E3 ubiquitin ligase abundance in the tender group were noticeably higher than those in the tough group at 12 h. There was a negative correlation between the shear force and desmin degradation. The desmin degradation was negatively correlated with desmin phosphorylation and ubiquitination levels. The phosphorylation level of desmin was positively correlated with its ubiquitination. In summary, this study suggests that AMPK and E3 ubiquitin ligase concurrently play significant roles in regulating meat tenderness by regulating phosphorylation and ubiquitination in meat postmortem.

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