数字农科院2.0

Enrichable cross-linkers for mapping direct protein interactions

文献类型： 外文期刊

作者： Ting Wu;Hang Xu Zhou;Jing Tian;Rong Zhou;Shangwei Huangfu;Bi Kun Jin;Anna Sablina;Fangfang Zhou;Hongli Chen;Shibing Tang;Long Zhang;Bing Yang

作者机构：

关键词： Cross-linking mass spectrometry;Direct protein interaction;IMAC;Subcellular organelles;Thioredoxin

期刊名称： Genome Biology

ISSN： 1474-760X

年卷期： 2025 年 26 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Background: It is crucial to investigate protein functions in specific subcellular environments. Cross-linking mass spectrometry is a powerful tool to map the direct interactome of proteins by identifying inter-protein cross-links. However, it is challenging to identify inter-protein cross-linked peptides due to their low abundance. Results: We chemically synthesize the cross-linkers ePDES1 and ePDES2 with an alkyne group and a compound with azide linked to a phosphate group to enrich for cross-linked peptides. Conclusion: Based on the high-quality cross-linking spectra of ePDES1 and ePDES2, our methods achieve the identification of hundreds of directly interacting proteins or substrates of thioredoxins in the nucleus and mitochondria.

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