数字农科院2.0

Nucleic acid-based reference materials: A “ruler” for precision molecular diagnostics

文献类型: 外文期刊

作者: Kai Li;Yaning Guo;Xin Qi;Fukai Li;Xinran Li;Mengrui Yang;Jian Zhou;Haiwei Zhou;Gang Liu;Liang Li

作者机构:

关键词: Analytical chemistry;COVID-19;Molecular detection;Reference materials

期刊名称: TrAC - Trends in Analytical Chemistry

ISSN: 0165-9936

年卷期: 2025 年 189 卷

页码:

收录情况: SCIE(2025版) ; ; EI(2025版)

摘要: Analytical chemistry is experiencing a growing demand for reliable and traceable results, which have significant implications for public health and food safety. The COVID-19 pandemic further underscores the need for accurate and dependable molecular diagnostics. Nucleic acid-based reference materials (NARMs) play a crucial role in ensuring the quality of results. Notably, NARMs have gained increased attention following the outbreak of COVID-19. While previous reviews introduced NARMs but overlooked their applications in different fields. Moreover, the integration of multiple methods represents a new trend in the preparation of NARMs, but these have not appeared in previous reviews. Therefore, this review delineates NARMs classification, synthesizes characterization techniques, reviews their applications in virus diagnosis, animal diseases, pathogenic microorganisms, genetic diseases and genetically modified organism detection, and provides a perspective on NARMs’ development and current challenges. In-depth study of NARMs will be significant for precise molecular diagnostics in biology, agriculture and public health.

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