数字农科院2.0

Enhancing lactic acid production by organic loading rate regulation during anaerobic co-fermentation: Insights into metabolic and bacterial responses

文献类型： 外文期刊

作者： Tianjing Lian;Fubin Yin;Qitao Cao;Tanlong Zhou;Fangyu Zhang;Jiaxin Sun;Yangyang Cai;Hongmin Dong

作者机构：

关键词： Anaerobic co-fermentation;Apple waste;Lactic acid;Organic loading rate;Swine manure

期刊名称： Environmental Technology and Innovation

ISSN： 2352-1864

年卷期： 2025 年 40 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Anaerobic co-fermentation (AcoF) offers an efficient approach to convert swine manure (SM) and apple waste (AW) into valuable products such as lactic acid (LA), promoting resource recovery and sustainable utilization. This study investigated how stepwise increases in organic loading rate (OLR) influenced LA production at a temperature of 35 ℃, revealing the associated microbial and metabolic responses during semi-continuous AcoF of SM and AW. The results demonstrated that increasing the OLR to 7 g volatile solids (VS)/L/d significantly enhanced LA accumulation to a concentration of 35.70 ± 1.08 g/L, achieved with a hydraulic retention time (HRT) of 10 d and a substrate mixing ratio of SM:AW= 40:60, which was 1.92 times higher than that observed at 5 g VS/L/d. The initial pH declined sharply and then stabilized around 3.80. Moreover, in-depth studies demonstrated that increasing OLR greatly improved SM and AW disintegration, with hydrolysis and acidification rate reaching 72.60 ± 5.62 % and 83.22 ± 2.92 %, respectively, under an OLR of 7 g VS/L/d, thereby enhancing substrate conversion efficiency. Microbial community analysis revealed that functional bacterial (i.e., Clostridium_sensu_stricto_1 and Terrisporobacter) associated with hydrolysis were enriched at high OLR, providing sufficient substrates for Lactobacillus to metabolize and produce LA. Metagenomic analysis revealed that an elevated relative abundance of carbohydrate metabolism and membrane transport functions under an OLR of 7 g VS/L/d promotes LA accumulation. This research could offer a technological foundation for efficient large-scale LA production using agricultural waste.

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