数字农科院2.0

Tissue distribution and properties of glutathione S-transferases in Apis cerana cerana Fabricius and Apis mellifera ligustica Spinola

文献类型： 外文期刊

作者： Diao, Qingyun;Yuan, Kai;Liang, Pei;Gao, Xiwu

作者机构：

关键词： Apis cerana cerana;Apis mellifera ligustica;glutathione S-transferases;kinetic characteristics;developmental stages

期刊名称： JOURNAL OF APICULTURAL RESEARCH

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年卷期：

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收录情况： SCI

摘要： The glutathione S-transferases (GSTs) have been recognized to play an important role in the detoxification of xenobiotics. The tissue distribution and activities of GSTs in Apis cerana cerana Fabricius and Apis mellifera ligustica Spinola were assayedusing 1-chloro-2,4-dinitrobenzene (CDNB) as substrate. GSTs were present at all the developmental stages of honeybee and widely distributed in different organs. GSTs from the midgut of both species showed the highest activity among all the detected organs. The enzymes were preferentially present in cytosol fraction of midgut. GSTs activities of each organ in A. cerana were lower than those in A mellifera. In A. cerana, the activity and Michaelis Menten (K_m) value of GSTs increased along with the development, no significant difference of activity and K_m was observed among different tissues. The enzymes activities increased rapidly and reached a maximum at the pupal stage to 0.081 mu mol.min~(-1).mg~(-1) protein. A. mellifera samples represented a different pattern. Significant differences of activity and K_m value were observed in different tissues. The GSTs in the midgut had the highest K_m value. However, the highest activity was found in the larvae and the lowest in the adult The GSTs activitiesin drone were higher than those in worker bee. During the life span of A mellifera worker bee, enzymes activities fluctuated between 0.2 and 0.4 mu mol.min~(-1).mg~(-1) pro. from the first day to 20th day and reached a peak on day 21 of adulthood. Significant differences of specific activity and kinetic parameters of GSTs were observed among five honeybee subspecies. The highest activity was in A mellifera caucasica and the lowest in A cerana cerana. The GSTs from A mellifera anatolica had the highestK_m and those from caucasica had the lowest. In A mellifera, it was GSTs from anatolica that had the highest V_(max) and those from caucasica had the lowest. The GSTs from A cerana had a higher affinity for CDNB than those from A mellifera.

分类号： S8

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