数字农科院2.0

Royal jelly proteins-derived anti-inflammatory peptides protected inflammation in DSS-induced colitis mice through Src/NF-κB signaling pathway

文献类型： 外文期刊

作者： Jianying Guo;Ruihua Ye;Qingyun Guo;Fei Pan;Zixu Wang;Yaoxing Chen;Wenli Tian;Yulan Dong

作者机构：

关键词： Anti-Inflammatory peptides;Colitis;Hydrolysates;Intestinal mucosal barrier;Macrophages;Major royal jelly proteins

期刊名称： Food Bioscience

ISSN： 2212-4292

年卷期： 2025 年 74 卷

页码：

收录情况： SCIE（2025版） ; ; 农林核心（2024版）

摘要： Royal jelly (RJ) has shown good curative effects in gastrointestinal diseases, diabetes, and other diseases because of its rich active ingredients. The proteolytic reaction hydrolyzes the proteins in RJ into peptides, which improves the biological function of RJ and its proteins. Inflammatory bowel disease (IBD) is a chronic inflammatory disease. With the increasing incidence of ulcerative colitis in Asia, IBD has a huge impact on the world. This study further investigated the protective effect of hydrolysates of RJ and major royal jelly proteins (MRJPs) on Dextran Sulfate Sodium (DSS)-induced colitis and the possible bioactive peptides in hydrolysates. We found that compared to RJ and MRJPs alone, the hydrolysates of RJ (1 g/kg) and MRJPs (200 mg/kg) obtained through alkaline protease and proteinase K further reduced mucosal barrier injury and suppressed inflammation by inhibiting the polarization of M1 type macrophages, the activation of the NF-κB signaling pathway, and the release of inflammatory factors. Among them, the proteinase K hydrolysate of MRJPs showed a better effect in alleviating colitis in mice. LC-MS/MS and PREDAIP were used to screen out four non-toxic anti-inflammatory peptides from the fifteen peptides with higher content in the proteinase K hydrolysis products of RJ and MRJPs. Network pharmacology, molecular docking, and cell experiments revealed that peptide KFFDY may mainly inhibit colitis by interacting with the target Src, influencing its phosphorylation and subsequently regulating the TLR4/NF-κB signaling pathway.

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