数字农科院2.0

A strain defined as a novel species in the Acinetobacter genus co-harboring chromosomal associated tet(X3) and plasmid associated blaNDM-1 from a beef cattle farm in Hebei, China

文献类型： 外文期刊

作者： Wang, Qing;Wei, Yanming;Shoaib, Muhammad;Qiu, Yanhua;Zhang, Chao;Dai, Guonian;Lin, Honglin;Wang, Weiwei;Zhang, Jiyu

作者机构：

关键词： antibiotic resistance genes (ARGs);tet(X);blaNDM;Acinetobacter;multi-drug resistance;pdif module

期刊名称： FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

ISSN： 2235-2988

年卷期： 2025 年 15 卷

页码：

收录情况： SCIE（2025版）

摘要： Introduction The co-existence phenomenon of antibiotic resistance genes (ARGs), particularly of last-resort antibiotics in multi-drug resistant (MDR) bacteria, is of particular concern in the least studied bacterial species.Methods In 2023, strain M2 was isolated from the sludge sample at a commercial bovine farm in Hebei province, China, using a MacConkey plate containing meropenem. PCR amplification and Sanger sequencing verified it co-carrying bla NDM and tet(X) genes. It was classified within the Acinetobacter genus by MALDI-TOF-MS and 16S rDNA analyses. Whole-genome sequencing (WGS) was performed on the Oxford Nanopore platform, with species-level identification via ANI and dDDH. Antimicrobial susceptibility testing was performed against 20 antibiotics. Conjugation assays employed the filter-mating method using E. coli J53 and Salmonella LGJ2 as recipients.Results This strain was confirmed as a novel species of Acinetobacter genus, showing resistance to meropenem, ampicillin, ceftazidime, cefepime, gentamicin, kanamycin, fosfomycin, imipenem, ertapenem, and tetracycline. Despite carrying tet(X3), it remained susceptible to tigecycline, omadacycline, and doxycycline. The genome carried 11 ARG types, multiple metal resistance genes (MRGs), and virulence factor (VF) genes. The bla NDM-1 was located in a skeleton, ISAba125-bla NDM-1-ble MBL-trpF, which was carried by an ISAba14-mediated rolling-circle-like structure in pM2-2-NDM-1 (rep_cluster_481). Integrative and conjugative element (ICE) and multiple pdif modules (driven by the XerCD site-specific recombination (XerCD SSR) system), which were associated with the mobilization of resistance determinants, were identified in this plasmid. Chromosomal tet(X3) was mediated by ISVsa3, forming a skeleton, ISVsa3-XerD-tet (X3)-res-ISVsa3.Discussion The co-occurrence of bla NDM and tet(X) in a novel species of the Acinetobacter genus hints that substantial undiscovered bacteria co-carrying high-risk ARGs are concealing in the agroecological system, which should cause particular concern.

分类号：

  • 相关文献

[1]The emergence of multi-drug resistant and virulence gene carrying Escherichia coli strains in the dairy environment: a rising threat to the environment, animal, and public health. Shoaib, Muhammad,He, Zhoulin,Geng, Xiang,Tang, Minjia,Hao, Ruochen,Wang, Shengyi,Shang, Ruofeng,Wang, Xuehong,Zhang, Hongjuan,Pu, Wanxia. 2023

[2]一株与奶牛子宫内膜炎相关的鲍曼不动杆菌的分离与鉴定. 王旭荣,王孝武,李建喜,王学智,杨志强. 2014

[3]鱼类消化道微生物——从结构到功能. 周志刚. 2012

[4]New Characterization of Multi-Drug Resistance of Streptococcus suis and Biofilm Formation from Swine in Heilongjiang Province of China. Dong C.-L.,Che R.-X.,Wu T.,Qu Q.-W.,Chen M.,Zheng S.-D.,Cai X.-H.,Wang G.,Li Y.-H.. 2023

[5]Transferability of tigecycline resistance: Characterization of the expanding Tet(X) family. Cui, Chao-Yue,Chen, Qiwei,He, Qian,Chen, Chong,Zhang, Rong-Min,Feng, Youjun,Sun, Jian. 2021

[6]Effect of Bacterial Resistance of Escherichia coli From Swine in Large-Scale Pig Farms in Beijing. Liu X.,Liu Q.,Cheng Y.,Liu R.,Zhao R.,Wang J.,Wang Y.,Yang S.,Chen A.. 2022

[7]Novel ST3871 Escherichia coli exhibits diverse transmission modes, carrying the tet(X4) resistance gene. Wang, Qing,Wang, Chengye,Shoaib, Muhammad,Yao, Xiaohui,Chen, Xi,Qiu, Yanhua,Dai, Guonian,Lin, Honglin,Wang, Weiwei,Zhang, Jiyu. 2026

[8]Diversity of degradation pathways of some aromatic compounds by phenotype and genotype testing in Acinetobacter strains. Yu, XQ,Belhaj, A,Elmerich, C,Lin, M. 2004

[9]Indole Degradation In A Model S.ystem And In Poultry M anure By Acinetobacter Spp.. Tesso, TA, Liu, GH. 2019

[10]Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.. T. A. Tesso,G. Liu. 2023

[11]A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism. Shuanghu Fan,Jingjing Guo,Shaoyan Han,Haina Du,Zimeng Wang,Yajuan Fu,Hui Han,Xiaoqiang Hou,Weixuan Wang. 2023

[12]The Roles of Plant-Growth-Promoting Rhizobacteria (PGPR)-Based Biostimulants for Agricultural Production Systems. Wenli Sun,Mohamad Hesam Shahrajabian,Ali Soleymani. 2024

[13]Gut Microbe-Driven Resistance Mechanisms in Propylea Japonica: Insights from Horizontal Gene Transfer and Oxidative Phosphorylation. Huangfu, Ningbo,Zhu, Xiangzhen,Tang, Zhijuan,Wang, Li,Zhang, Kaixin,Li, Dongyang,Ji, Jichao,Cui, Jinjie,Guo, Zhaojiang,Luo, Junyu,Gao, Xueke. 2025

[14]Impact of different manure-derived dissolved organic matters on the fate of arsenic-antibiotic in co-contaminated paddy soils. Mengmeng Yan,Changxiong Zhu,Tingting Song,Binxu Li,Shiming Su,Hongna Li. 2022

[15]Manure application led to higher antibiotic resistance risk in red soil compared with black soil and fluvo-aquic soil. Tingting Song,Changxiong Zhu,Binxu Li,Mengmeng Yan,Hongna Li. 2023

[16]Insights into antibiotic resistance-related changes in microbial communities, resistome and mobilome in paddy irrigated with reclaimed wastewater. Qing Yan,Yufeng Xu,Zhengzheng Zhong,Yuan Xu,Xiaoyan Lin,Zhaoyun Cao,Guozhong Feng. 2023

[17]Overlooked interconversion between tetracyclines and their 4-epimers in soil and effects on soil resistome and bacterial community. Chenxi Lu,Cheng Qin,Lixia Zhao,Huike Ye,Mohan Bai,Yang Sun,Xiaojing Li,Liping Weng,Yongtao Li. 2024

[18]Polyvinyl chloride microplastics disseminate antibiotic resistance genes in Chinese soil: A metagenomic analysis. Shuwen Zhao,Qianru Zhang,Qilan Huang,Chuchen Zhang,Hongna Li,Kadambot H.M. Siddique. 2024

[19]Unveiling the sustained effects of plant root exudates on soil microbiome and resistome and the related functional traits. Lu Tan,Jiayin Liang,Ziyi Qin,Tianyang Ning,Xiaocheng Wei,Bo Yang,Qiang Wang,Yan Xu,Feng Shen. 2025

作者其他论文 更多>>