数字农科院2.0

Physiological and Differential Proteomic Analysis at Seedling Stage by Induction of Heavy-Ion Beam Radiation in Wheat Seeds

文献类型： 外文期刊

作者： Li, Yuqi;Gu, Jiayu;Irshad, Ahsan;Zhao, Linshu;Guo, Huijun;Xiong, Hongchun;Xie, Yongdun;Zhao, Shirong;Ding, Yuping;Zhou, Libin;Kong, Fuquan;Fang, Zhengwu;Liu, Luxiang

作者机构：

关键词： C-12 ion beam;Li-7 ion beam;proteomics;TMT;wheat

期刊名称： FRONTIERS IN GENETICS

ISSN：

年卷期： 2022 年 13 卷

页码：

摘要： Novel genetic variations can be obtained by inducing mutations in the plant which help to achieve novel traits. The useful mutant can be obtained through radiation mutation in a short period which can be used as a new material to produce new varieties with high yield and good quality wheat. In this paper, the proteomic analysis of wheat treated with different doses of C-12 and Li-7 ion beam radiation at the seedling stage was carried out through a Tandem Mass Tag (TMT) tagging quantitative proteomic analysis platform based on high-resolution liquid chromatography-mass spectrometry, and the traditional Co-60-gamma-ray radiation treatment for reference. A total of 4,764 up-regulated and 5,542 down-regulated differentially expressed proteins were identified. These proteins were mainly enriched in the KEGG pathway associated with amino acid metabolism, fatty acid metabolism, carbon metabolism, photosynthesis, signal transduction, protein synthesis, and DNA replication. Functional analysis of the differentially expressed proteins showed that the oxidative defense system in the plant defense system was fully involved in the defense response after C-12 ion beam and Li-7 ion beam radiation treatments. Photosynthesis and photorespiration were inhibited after C-12 ion beam and Co-60-gamma-ray irradiation treatments, while there was no effect on the plant with Li-7 ion beam treatment. In addition, the synthesis of biomolecules such as proteins, as well as multiple signal transduction pathways also respond to radiations. Some selected differentially expressed proteins were verified by Parallel Reaction Monitoring (PRM) and qPCR, and the experimental results were consistent with the quantitative results of TMT. The present study shows that the physiological effect of C-12 ion beam radiation treatment is different as compared to the Li-7 ion beam, but its similar to the Co-60-gamma ray depicting a significant effect on the plant by using the same dose. The results of this study will provide a theoretical basis for the application of C-12 and Li-7 ion beam radiation in the mutation breeding of wheat and other major crops and promote the development of heavy ion beam radiation mutation breeding technology.

分类号：

  • 相关文献

[1]充分发掘保护利用生物多样性;促进农业可持续发展——《生物多样性与小麦改良》简评. 李祥洲. 1997

[2]iTRAQ-based quantitative proteomic analysis of wheat roots in response to salt stress. Jiang, Qiyan,Niu, Fengjuan,Sun, Xianjun,Hu, Zheng,Zhang, Hui,Li, Xiaojuan.

[3]30个小麦新品系抗条锈病基因分析及成株期抗病性评价. 冯晶,蔺瑞明,林凤,徐世昌. 2013

[4]小麦抗菌蛋白编码基因BS108的克隆及其转基因抗病小麦的创制. 李钊,周淼平,张增艳,庄洪涛,徐惠君,杜丽璞,叶兴国. 2010

[5]渗透调节物质及有机附加物对小麦体细胞胚发生影响的研究(英文). 孙朝霞,侯思宇,王玉国. 2008

[6]普通小麦品种辐射敏感性模糊聚类分析<英文>. 冯志杰,王琳清. 1992

[7]基因枪转化小麦主要轰击参数的优化. 闵东红,何莎,张彦,夏兰琴. 2013

[8]小麦矮缩病毒分子群体遗传结构研究. 刘艳,王锡锋,周广和. 2008

[9]Effects of Different Substrates on Lignocellulosic Enzyme Expression, Enzyme Activity, Substrate Utilization and Biological Efficiency of Pleurotus Eryngii. Xie, Chunliang,Yan, Li,Gong, Wenbing,Zhu, Zuohua,Tan, Senwei,Chen, Du,Hu, Zhenxiu,Peng, Yuande. 2016

[10]Comparative proteomic analysis reveals alterations in development and photosynthesis-related proteins in diploid and triploid rice. Shuzhen Wang (2), Wenyue Chen +, Changdeng Yang , Jian Yao , Wenfei Xiao , Ya Xin , Jieren Qiu , Weimin Hu , Haigen Yao , Wu Ying , Yaping Fu , Jianxin Tong , Zhongzhong Chen , Songlin Ruan *, Huasheng Ma *. 2016

[11]Proteomic Analysis Reveals the Effects of Different Dietary Protein Levels on Growth and Development of Jersey-Yak. Guowu Yang,Rongfeng Dai,Xiaoming Ma,Chun Huang,Xiaoyong Ma,Xinyi Li,Yongfu La,Renqing Dingkao,Ji Renqing,Xian Guo,Ta Zhaxi,Chunnian Liang. 2024

[12]Quantitative phosphoproteomic analysis of chicken DF-1 cellsinfected with Eimeria tenella, using tandem mass tag (TMT) andparallel reaction monitoring (PRM) mass spectrometry. Liu Shu Jia,Zhan Liu,Shun Hai Zhu,Qi Ping Zhao,Hong Yu Han,Huan Zhi Zhao,Yu Yu,Hui Dong. 2024

[13]Comparative proteomics analysis of pomegranate seeds on fruit maturation period (Punica granatum L.). CAO Shang-yin,Niu Juan,Cao Da,Li Haoxian,Xue Hui,Chen Lina,Zhang Fuhong,ZHAO Di-guang. 2015

[14]PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER). Chen, Li-Zhen,Liang, Ge-Mei,Zhang, Jie,Wu, Kong-Ming,Guo, Yu-Yuan,Rector, Brian G.. 2010

[15]Comparative Proteomic Analysis Shows an Elevation of Mdh1 Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats. Dong Shu-wei,Gao Zhao-hui,Li Xia,Shen Xiao-yun,Gao Zhao-hui,Xue Hui-wen. 2014

[16]Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine. Chen, Xiuping,Wang, Jiamei,Zhu, Haojun,Peng, Yufa,Wang, Jiamei,Zhu, Haojun,Ding, Jiatong. 2015

[17]Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains. Zhang, C. X.,Zhao, X.,Han, F.,Yang, M. F.,Chen, H.,Shen, S. H.,Zhang, C. X.,Chida, T.. 2009

[18]Metabolic responses and "omics" technologies for elucidating the effects of heat stress in dairy cows. Min, Li,Zhao, Shengguo,Tian, He,Zhou, Xu,Zhang, Yangdong,Li, Songli,Zheng, Nan,Wang, Jiaqi,Min, Li,Yang, Hongjian.

[19]Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis. Xue, Junxin,Xiao, Yan,Huang, Kehe,Xue, Junxin,Jiang, Wei,Chen, Yongjun,Liu, Yingchun,Zhang, Huajing,Wang, Quan,Qiao, Yuanbiao. 2016

[20]A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat. He, Xue,Fang, Jingjing,Li, Jingjuan,Qu, Baoyuan,Ren, Yongzhe,Ma, Wenying,Zhao, Xueqiang,Li, Bin,Wang, Daowen,Li, Zhensheng,Tong, Yiping,Fang, Jingjing,Li, Jingjuan,Ren, Yongzhe. 2014

作者其他论文 更多>>