数字农科院2.0

Occludin and collagen IV degradation mediated by the T9SS effector SspA contributes to blood-brain barrier damage in ducks during Riemerella anatipestifer infection

文献类型： 外文期刊

作者： Chen Z.;Zhu M.;Liu D.;Wu M.;Niu P.;Yu Y.;Ding C.;Yu S.

作者机构：

关键词： Riemerella anatipestifer;blood‒brain barrier;collagen IV;occludin;SspA;T9SS

期刊名称： Veterinary research

ISSN： 1297-9716

年卷期： 2024 年 55 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： Riemerella anatipestifer infection is characterized by meningitis with neurological symptoms in ducklings and has adversely affected the poultry industry. R. anatipestifer strains can invade the duck brain to cause meningitis and neurological symptoms, but the underlying mechanism remains unknown. In this study, we showed that obvious clinical symptoms, an increase in blood‒brain barrier (BBB) permeability, and the accumulation of inflammatory cytokines occurred after intravenous infection with the Yb2 strain but not the mutant strain Yb2ΔsspA, indicating that Yb2 infection can lead to cerebrovascular dysfunction and that the type IX secretion system (T9SS) effector SspA plays a critical role in this pathological process. In addition, we showed that Yb2 infection led to rapid degradation of occludin (a tight junction protein) and collagen IV (a basement membrane protein), which contributed to endothelial barrier disruption. The interaction between SspA and occludin was confirmed by coimmunoprecipitation. Furthermore, we found that SspA was the main enzyme mediating occludin and collagen IV degradation. These data indicate that R. anatipestifer SspA mediates occludin and collagen IV degradation, which functions in BBB disruption in R. anatipestifer-infected ducks. These findings establish the molecular mechanisms by which R. anatipestifer targets duckling endothelial cell junctions and provide new perspectives for the treatment and prevention of R. anatipestifer infection. © 2024. The Author(s).

分类号：

  • 相关文献

[1]Riemerella anatipestifer T9SS Effector SspA Functions in Bacterial Virulence and Defending Natural Host Immunity. Chen, Zongchao,Niu, Pengfei,Ren, Xiaomei,Han, Wenlong,Shen, Ruyu,Zhu, Min,Yu, Yang,Ding, Chan,Yu, Shengqing. 2022

[2]Genome-Wide Analysis and Characterization of the Riemerella anatipestifer Putative T9SS Secretory Proteins with a Conserved C-Terminal Domain. Chen, Zongchao,Niu, Pengfei,Ren, Xiaomei,Han, Wenlong,Shen, Ruyu,Zhu, Min,Yu, Yang,Yu, Shengqing. 2022

[3]Riemerella anatipestifer Endonuclease I displays enzymatic activity and is associated with bacterial virulence. Ruyu Shen,Yang Yu,Zongchao Chen,Min Zhu,Yating Feng,Pengfei Niu,Shengqing Yu. 2023

[4]A Riemerella anatipestifer Metallophosphoesterase That Displays Phosphatase Activity and Is Associated with Virulence. Pengfei Niu,Zongchao Chen,Xiaomei Ren,Wenlong Han,Hongyan Dong,Ruyu Shen,Chan Ding,Shanyuan Zhu,Shengqing Yu. 2021

[5]紧密连接蛋白occludin和claudin-1在猪体内不同组织的分布研究. 骆晓蕾,王玉娥. 2015

[6]紧密连接蛋白occludin和claudin-1在猪机体不同组织的分布研究. 骆晓蕾,郭龙军,谷伟红,冯力,王玉娥. 2016

[7]柔嫩艾美耳球虫感染对雏鸡肠道紧密连接相关蛋白表达的影响. 张艳冰,许笑,古少鹏,蔡建平. 2020

[8]Enhancement of blood-brain barrier permeability is required for intravenously administered virus neutralizing antibodies to clear an established rabies virus infection from the brain and prevent the development of rabies in mice. Huang, Chien-Tsun,Li, Zhenguang,Huang, Ying,Zhang, Guoqing,Zhou, Ming,Chai, Qingqing,Fu, Zhen F.,Li, Zhenguang,Wu, Hua,Fu, Zhen F..

[9]Tight Junction Protein Occludin Is a Porcine Epidemic Diarrhea Virus Entry Factor. Luo, Xiaolei,Guo, Longjun,Zhang, Jian,Xu, Yunfei,Gu, Weihong,Feng, Li,Wang, Yue.

[10]Effects of dietary administering chitosan on growth performance, jejunal morphology, jejunal mucosal sIgA, occluding, claudin-1 and TLR4 expression in weaned piglets challenged by enterotoxigenic Escherichia coli. Xiao, Dingfu,Zhang, Bin,Xiao, Dingfu,Yin, Yulong,Feng, Zemeng,Tang, Zhiru,Hu, Xionggui,Wang, Jinquan. 2013

[11]Development of the Blood-Brain Barrier in Ducks. Yang, Sheng,Wu, Jingxian,Shi, Yonghong,Huang, Yufei,Zhang, Yafei,Chen, Qiusheng. 2022

[12]PHD3 inhibits colon cancer cell metastasis through the occludin-p38 pathway. Yuyao Li,Tanglong Yuan,Hongwei Zhang,Shuting Liu,Jie Lun,Jing Guo,Yu Wang,Yuying Zhang,Jing Fang. 2023

[13]Development of multiplex PCR assay for rapid detection of Riemerella anatipestifer, Escherichia coli, and Salmonella enterica simultaneously from ducks. Hu, Qinghai,Tu, Jing,Han, Xiangan,Zhu, Yinyu,Ding, Chan,Yu, Shengqing,Ding, Chan,Yu, Shengqing. 2011

[14]Construction of a shuttle vector for use in Riemerella anatipestifer. Hu, Qinghai,Miao, Shuang,Ni, Xintao,Lu, Fengying,Yu, Hui,Xing, Linlin,Jiang, Pan. 2013

[15]Genome-wide mining of potential virulence-associated genes in Riemerella anatipestifer using random transposon mutagenesis. Ni, Xintao,Jiang, Pan,Xing, Linlin,Ou, Changcan,Yu, Hui,Qi, Jingjing,Sun, Bingqing,Cui, Junsheng,Hu, Qinghai,Wang, Guijun.

[16]The M949_1556 gene plays a role on the bacterial antigenicity and pathogenicity of Riemerella anatipestifer. Zou, Jiechi,Wang, Xiaolan,Tian, Mingxing,Cao, Shoulin,Hou, Wanwan,Wang, Shaohui,Han, Xiangan,Ding, Chan,Yu, Shengqing.

[17]Characterization and cross-protection evaluation of M949_1603 gene deletion Riemerella anatipestifer mutant RA-M1. Zou, Jiechi,Wang, Xiaolan,Ding, Chan,Tian, Mingxing,Han, Xiangan,Wang, Shaohui,Yu, Shengqing.

[18]Effects of two efflux pump inhibitors on the drug susceptibility of Riemerella anatipestifer isolates from China. Li Ya-fei,Jiang Hong-xia,Zhang Ya-nan,Zhao Li-qing,Gu Peng,Wang Li-qiao,Zeng Zhen-ling,Xiang Rong,Sun Na. 2016

[19]Immunoproteomics analysis of whole cell bacterial proteins of Riemerella anatipestifer. Hu, Qinghai,Ding, Chan,Tu, Jing,Wang, Xiaolan,Han, Xiangan,Duan, Yunbing,Yu, Shengqing,Yu, Shengqing.

[20]Levamisole enhances immunity in ducklings vaccinated against Riemerella anatipestifer. Zhang, Yuewei,Zeng, Xiangfan,Wu, Wenxue,Chen, Huiling,Wang, Peng,Li, Jinxiang.

作者其他论文 更多>>