数字农科院2.0

Unveiling alfalfa root rot resistance genes through an integrative GWAS and transcriptome study

文献类型： 外文期刊

作者： He, Fei;Xu, Ming;Liu, Hao;Xu, Yanchao;Long, Ruicai;Kang, Junmei;Yang, Qingchuan;Chen, Lin

作者机构：

关键词： Alfalfa;GWAS;RNA-seq;Root rot

期刊名称： BMC PLANT BIOLOGY

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： BackgroundRoot rot is a major disease affecting alfalfa (Medicago sativa L.), causing significant yield losses and economic damage. The primary pathogens include Fusarium spp., Rhizoctonia spp., Pythium spp., and Phytophthora spp., with Fusarium being particularly severe. Breeding disease-resistant varieties is crucial for mitigating these losses.ResultsUnder conditions of inoculation with Fusarium oxysporum, we conducted a statistical analysis of six phenotypic traits in alfalfa. Significant phenotypic variation was observed among different alfalfa varieties. Correlation analysis revealed significant relationships among traits such as relative yield, relative plant height (PH), and relative root number (NR), indicating potential synergistic roles of these traits in disease resistance. Through GWAS analysis, we identified 41 significant single nucleotide polymorphisms (SNP) associated with root rot resistance across eight chromosomes. The transcriptome analysis identified multiple differentially expressed genes (DEGs) associated with root rot stress, including transcription factors such as WRKY, NAC, AP2, GRAS, HLH, B3, MYB, and ARF. By integrating GWAS and transcriptome data, we identified four key DEGs significantly associated with root rot resistance, offering valuable insights for developing disease-resistant alfalfa varieties and enhancing overall crop resilience.ConclusionOur study identified significant phenotypic variation and key correlations among traits under root rot stress in alfalfa. We pinpointed 41 significant SNPs associated with root rot resistance across eight chromosomes and identified several key DEGs, including WRKY, NAC, and MYB transcription factors. The integration of GWAS and RNA-Seq data identified four key DEGs associated with root rot resistance, providing valuable insights for breeding disease-resistant alfalfa varieties and enhancing crop resilience.

分类号：

  • 相关文献

[1]Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Medicago sativa L.). Fei He,Chunxue Wei,Yunxiu Zhang,Ruicai Long,Mingna Li,Zhen Wang,Qingchuan Yang,Junmei Kang,Lin Chen. 2022

[2]小麦芒基因定位及其与农艺性状的相关性分析. 李玲,刘盼,张蕾,张浩,贾继增,高丽锋. 2021

[3]Screening of Alfalfa Varieties Resistant to Phytophthora cactorum and Related Resistance Mechanism. Menghuan Tao,Yao Zhao,Tianxue Hu,Quan Zhang,Hui Feng,Yiwen Lu,Zhenfei Guo,Bo Yang. 2023

[4]A Genome-Wide Association Study Coupled With a Transcriptomic Analysis Reveals the Genetic Loci and Candidate Genes Governing the Flowering Time in Alfalfa (Medicago sativa L.). He F.,Zhang F.,Jiang X.,Long R.,Wang Z.,Chen Y.,Li M.,Gao T.,Yang T.,Wang C.,Kang J.,Chen L.,Yang Q.. 2022

[5]Transcriptome and GWAS Analyses Reveal Candidate Gene for Root Traits of Alfalfa during Germination under Salt Stress. Fei He,Tianhui Yang,Fan Zhang,Xueqian Jiang,Xianyang Li,Ruicai Long,Xue Wang,Ting Gao,Chuan Wang,Qingchuan Yang,Lin Chen,Junmei Kang. 2023

[6]Genome-Wide Association Study (GWAS) Identifies Key Candidate Genes Associated with Leaf Size in Alfalfa (Medicago sativa L.). Xu M.,Jiang X.,He F.,Sod B.,Yang T.,Zhang F.,Cong L.,Long R.,Li M.,Wang X.,Yang Q.,Zhang T.,Kang J.. 2023

[7]Determining the Genetic Architecture and Breeding Potential of Quality Traits in Alfalfa (Medicago sativa L.) Through Genome-Wide Association Study and Genomic Prediction. Ming Xu,Kai Zhu,Xueqian Jiang,Fan Zhang,Bilig Sod,Huajuan Leng,Tian Zhang,Yanchao Xu,Tianhui Yang,Mingna Li,Xue Wang,Qingchuan Yang,Junmei Kang,Tiejun Zhang,Lin Chen,Ruicai Long,Fei He. 2025

[8]RAD-Seq-Based High-Density Linkage Maps Construction and Quantitative Trait Loci Mapping of Flowering Time Trait in Alfalfa (Medicago sativa L.). Jiang, Xueqian,Yang, Tianhui,Zhang, Fan,Yang, Xijiang,Yang, Changfu,He, Fei,Long, Ruicai,Gao, Ting,Jiang, Yiwei,Yang, Qingchuan,Wang, Zhen,Kang, Junmei. 2022

[9]Combining QTL mapping and RNA-Seq Unravels candidate genes for Alfalfa (Medicago sativa L.) leaf development. Xueqian Jiang,Xijiang Yang,Fan Zhang,Tianhui Yang,Changfu Yang,Fei He,Ting Gao,Chuan Wang,Qingchuan Yang,Zhen Wang,Junmei Kang. 2022

[10]Identification of QTL and candidate genes associated with biomass yield and Feed Quality in response to water deficit in alfalfa (Medicago sativa L.) using linkage mapping and RNA-Seq. Xueqian Jiang,Andong Yu,Fan Zhang,Tianhui Yang,Chuan Wang,Ting Gao,Qingchuan Yang,Long Xi Yu,Zhen Wang,Junmei Kang. 2022

[11]卫星搭载对紫花苜蓿当代植株生物量的影响(英文). 任卫波,赵亮,王蜜,陈立波,郭慧琴. 2008

[12]Dissecting the Genetic Mechanisms of Hemicellulose Content in Rapeseed Stalk. Xu, Yinhai,Yang, Yuting,Yu, Wenkai,Liu, Liezhao,Hu, Qiong,Wei, Wenliang,Liu, Jia. 2022

[13]Genome-wide association and RNA-seq analyses identify loci for pod orientation in rapeseed (Brassica napus). Yang Y.,Wang W.,Hu Q.,Raman H.,Liu J.. 2023

[14]Genome-wide association study for phosphate responsive root hair length and density in bread wheat. Saman Maqbool,Fatima Saeed,Ammarah Maqbool,Muhammad Ibrar Khan,Mohsin Ali,Awais Rasheed,Xianchun Xia,Zhonghu He. 2023

[15]Integrating GWAS and transcriptomics to identify candidate genes conferring relative growth rate trait in white-feathered broiler. Peihao Liu,Na Luo,Dawei Liu,Fan Ying,Dan Zhu,Jie Wen,Guiping Zhao,Bingxing An. 2024

[16]ZmBARK1 as a low-temperature tolerance gene in maize germination. Xu, Qingyu,Song, Yiying,Bao, Di,Meng, Lingzhi,Di, Hong,Zhang, Lin,Dong, Ling,Zeng, Xing,Zhang, Jiayue,Li, Chunxiang,Xing, Jiapeng,Zhang, Naifu,Li, Xin,Weng, Jianfeng,Wang, Zhenhua,Zhou, Yu. 2025

[17]Unraveling key genes and pathways involved in Verticillium wilt resistance by integrative GWAS and transcriptomic approaches in Upland cotton. Khan, Majid,Hu, Daowu,Dai, Shuai,Li, Hongge,Peng, Zhen,He, Shoupu,Awais, Muhammad,Du, Xiongming,Geng, Xiaoli. 2025

[18]Genome-Wide Association Integrating a Transcriptomic Meta-Analysis Suggests That Genes Related to Fat Deposition and Muscle Development Are Closely Associated with Growth in Huaxi Cattle. Cheng Li Liu,Tao Ren,Peng Cheng Ruan,Yong Fu Huang,Simone Ceccobelli,De Jun Huang,Lu Pei Zhang,Guang Xin E. 2025

[19]Domestication of Tartary Buckwheat Shaped a Regulatory Module for Seedling Salt Tolerance by Targeting the Magnesium Transporter Gene FtMGT2. Lu, Xiang,He, Yuqi,Weng, Wenfeng,Liu, Zebin,Gao, Yuanfen,Shi, Yaliang,Li, Wei,Lai, Dili,Zhao, Mengyu,Jha, Rintu,Zhao, Hui,Li, Guangsheng,Guan, Chaonan,Shao, Shuai,Ruan, Jingjun,Woo, Sun Hee,Ouyang, Yinan,Quinet, Muriel,Georgiev, Milen I.,Fernie, Alisdair R.,Hou, Congcong,Zhang, Kaixuan,Liu, Xu,Zhou, Meiliang. 2025

[20]Integrative genome-wide association study and transcriptomic analysis to identify candidate genes and pathways for heterophil and lymphocyte traits in white-feathered broilers. Jumei Zheng,Qiao Wang,John F. O'Grady,Zixuan Wang,Man Deng,Jin Zhang,Xiang Li,Yuqing Zhu,David E. MacHugh,Guiping Zhao. 2026

作者其他论文 更多>>