数字农科院2.0

Casein kinase GhCKA1 positively regulates cotton resistance to Verticillium wilt

文献类型： 外文期刊

作者： Luqi Chen;Lihong Zhao;Zili Feng;Feng Wei;Yalin Zhang;Heqin Zhu;Hongjie Feng;Jinglong Zhou

作者机构：

关键词： Casein kinase;Cotton;GhCKA1;Verticillium wilt

期刊名称： Plant Science

ISSN： 0168-9452

年卷期： 2025 年 355 卷

页码：

收录情况： SCIE（2025版）

摘要： Verticillium wilt is an important disease that seriously affects the quality and yield of cotton. Fungal vascular diseases caused by Verticillium dahliae hinders the sustainable development of cotton cultivation. The most effective strategy for managing Verticillium wilt in cotton involves identifying resistance genes, investigating resistance mechanisms, and developing resistant varieties. In the laboratory, in our previous work, V. dahliae strain Vd080 was inoculated into both disease-resistant and disease-susceptible cotton strains, followed by a comprehensive transcriptomic analysis. The findings confirms the correlation between the gene GhCKA1 and disease resistance. In this study, silencing GhCKA1 expression led to reduced levels of reactive oxygen species, callose, and xylem accumulation, thereby inhibiting various defense responses and reducing cotton resistance to V. dahliae. Furthermore, we observed increased resistance to pathogens in Arabidopsis thaliana overexpressing GhCKA1. Subcellular localization experiments in tobacco indicated that GhCKA1 is localized within the nucleus. GUS staining analysis showed that the expression of the GhCKA1 promoter was influenced by pathogenic microorganisms. Additionally, we found that GhCKA1 interacts with aspartic proteases, an important proteolytic enzymes that play significant roles in metabolism and biological regulation. In conclusion, GhCKA1 enhances the resistance of cotton to V. dahliae and interacted with GhAsp1. Therefore, GhCKA1 may be a suitable molecular target to improve the resistance of cotton to Verticillium wilt, and provide a new breeding method for cotton to resist Werticillium wilt.

分类号：

  • 相关文献

[1]Spectrum characteristics of cotton canopy infected with verticillium wilt and inversion of severity level. Wang, Keru,Li, Shaokun,Bai, Junhua,Chen, Bing,Wang, Keru,Li, Shaokun,Bai, Junhua,Xiao, Chunhua,Lai, Junchen,Wang, Jing. 2008

[2]Estimating Severity Level of Cotton Infected Verticillium Wilt Based on Spectral Indices of TM Image. Chen, Bing,Wang, Keru,Li, Shaokun,Xiao, Chunhua,Chen, Jianglu,Jin, Xiulinag,Wang, Keru,Li, Shaokun,Chen, Bing.

[3]Diversity of Endophytic Fungi from Different Verticillium-Wilt-Resistant Gossypium hirsutum and Evaluation of Antifungal Activity Against Verticillium dahliae In Vitro. Wang, Ling-Fei,Feng, Zi-Li,Zhao, Li-Hong,Shi, Yong-Qiang,Zhu, He-Qin.

[4]Genetic analysis of Verticillium wilt resistance in a backcross inbred line population and a meta-analysis of quantitative trait loci for disease resistance in cotton. Jinfa Zhang,Jiwen Yu,Wenfeng Pei,Xingli Li,Joseph Said,Mingzhou Song,Soum Sanogo. 2015

[5]Characterization, Expression, and Functional Analysis of a Novel NAC Gene Associated with Resistance to Verticillium Wilt and Abiotic Stress in Cotton. Wang, Weina,Geng, Shuaipeng,Sun, Quan,Long, Lu,Cai, Chaowei,Liu, Xin,Wang, Guanghao,Miao, Chen,Zhang, Xiao,Cai, Yingfan,Wang, Weina,Yang, Can,Yuan, Youlu,Du, Xiongming,Chu, Zongyan. 2016

[6]Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Xianpeng Meng,Fuguang Li,Chuanliang Liu,Chaojun Zhang,Zhixia Wu,Yajuan Chen.

[7]Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton. Long, Lu,Sun, Quan,Wang, Weina,Gao, Wei,Cai, Chaowei,Mo, Jianchuan,Cheng, Jieru,Zhang, Xiangrui,Liu, Yujia,Miao, Chen,Zhang, Xiao,Cai, Yingfan,Sun, Quan,Wang, Weina,Du, Xiongming,Shi, Yuzhen,Yuan, Youlu,Chu, Zongyan.

[8]Identification and characterization of the Bicupin domain family and functional analysis of GhBCD11 in response to verticillium wilt in cotton. Ying Sun,Yi Wang,Yalin Zhang,Nadeem Hasan,Na Yang,Yijing Xie,Canming Tang. 2023

[9]Defense Mechanisms of Cotton Fusarium and Verticillium Wilt and Comparison of Pathogenic Response in Cotton and Humans. Man M.,Zhu Y.,Liu L.,Luo L.,Han X.,Qiu L.,Li F.,Ren M.,Xing Y.. 2022

[10]Self-assembled thiophanate-methyl/star polycation complex prevents plant cell-wall penetration and fungal carbon utilization during cotton infection by Verticillium dahliae. Xiaofeng Su,Shuo Yan,Weisong Zhao,Haiyang Liu,Qinhong Jiang,Ying Wei,Huiming Guo,Meizhen Yin,Jie Shen,Hongmei Cheng. 2023

[11]Interactions between Verticillium dahliae and cotton: pathogenic mechanism and cotton resistance mechanism to Verticillium wilt. Zhu, Yutao,Zhao, Mei,Li, Taotao,Wang, Lianzhe,Liao, Chunli,Liu, Dongxiao,Zhang, Huamin,Zhao, Yanpeng,Liu, Lisen,Ge, Xiaoyang,Li, Bingbing. 2023

[12]The respiratory burst oxidase homolog protein d (Ghrbohd) positively regulates the cotton resistance to verticillium dahliae. Wanting Huang,Yalin Zhang,Jinglong Zhou,Feng Wei,Zili Feng,Lihong Zhao,Yongqiang Shi,Hongjie Feng,Heqin Zhu. 2021

[13]Integrative Analysis of Expression Profiles of mRNA and MicroRNA Provides Insights of Cotton Response to Verticillium dahliae. Jun Mei,Yuqing Wu,Qingqing Niu,Meng Miao,Diandian Zhang,Yanyan Zhao,Fangfang Cai,Dongliang Yu,Liping Ke,Hongjie Feng,Yuqiang Sun. 2022

[14]Cotton CC-NBS-LRR Gene GbCNL130 Confers Resistance to Verticillium Wilt Across Different Species. Tinggang Li,Qianqian Zhang,Xilong Jiang,Ran Li,Nikhilesh Dhar. 2021

[15]Soil bacterial community response to continuous cropping of cotton. Zheng Ma,Peng Li,Chuanzhen Yang,Zili Feng,Hongjie Feng,Yalin Zhang,Lihong Zhao,Jinglong Zhou,Heqin Zhu,Feng Wei. 2023

[16]Overexpression of cotton Trihelix transcription factor GhGT-3b_A04 enhances resistance to Verticillium dahliae and affects plant growth in Arabidopsis thaliana. Haoming Mao,Wenqing Zhang,Junyuan Lv,Jiaxiang Yang,Shuxian Yang,Bing Jia,Jikun Song,Man Wu,Wenfeng Pei,Jianjiang Ma,Bingbing Zhang,Jinfa Zhang,Li Wang,Jiwen Yu. 2023

[17]Dynamic characteristics and functional analysis provide new insights into the role of GauERF105 for resistance against Verticillium dahliae in cotton. Yanqing Wang,Muhammad Jawad Umer,Xiaoyan Cai,Mengying Yang,Yuqing Hou,Yanchao Xu,Raufa Batool,Teame Gereziher Mehari,Jie Zheng,Yuhong Wang,Heng Wang,Zhikun Li,Zhongli Zhou,Fang Liu. 2023

[18]Genetic control of rhizosphere microbiome of the cotton plants under field conditions. Feng Wei,Zili Feng,Chuanzhen Yang,Lihong Zhao,Yalin Zhang,Jinglong Zhou,Hongjie Feng,Heqin Zhu,Xiangming Xu. 2024

[19]Functional verification of GhAFP8 in upland cotton under verticillium wilt stress. Ting Zhou,Peilin Wang,Jianglin Xu,Dong Liang,Yejun Yang,Yongqiang Wang,Yuanchun Pu,Guoli Zhang,Jinling Huang,Guoqing Sun. 2024

[20]Temperature impacts on cotton yield superposed by effects on plant growth and verticillium wilt infection in China. Tianyi Zhang,Zongming Xie,Jinglong Zhou,Hongjie Feng,Tao Zhang. 2024

作者其他论文 更多>>