数字农科院2.0

First Detection of NADC34-like PRRSV as a Main Epidemic Strain on a Large Farm in China

文献类型： 外文期刊

作者： Li, Chao;Gong, Bangjun;Sun, Qi;Xu, Hu;Zhao, Jing;Xiang, Lirun;Tang, Yan-Dong;Leng, Chaoliang;Li, Wansheng;Guo, Zhenyang;Fu, Jun;Peng, Jinmei;Wang, Qian;Zhou, Guohui;Yu, Ying;Meng, Fandan;An, Tongqing;Cai, Xuehui;Tian, Zhi-Jun;Zhang, Hongliang

作者机构：

关键词： NADC34-like PRRSV;main epidemic strains;100 aa deletion;RFLP

期刊名称： PATHOGENS

ISSN：

年卷期： 2022 年 11 卷 1 期

页码：

收录情况： JCR（2021版）

摘要： The newly emerged sublineage 1.5 (NADC34-like) porcine reproductive and respiratory syndrome virus (PRRSV) has posed a direct threat to the Chinese pig industry since 2018. However, the prevalence and impact of NADC34-like PRRSV on Chinese pig farms is unclear. In the present study, we continuously monitored pathogens-including PRRSV, African swine fever virus (ASFV), classical swine fever virus (CSFV), pseudorabies virus (PRV), and porcine circovirus 2 (PCV2)-on a fattening pig farm with strict biosecurity practices located in Heilongjiang Province, China, from 2020 to 2021. The results showed that multiple types of PRRSV coexisted on a single pig farm. NADC30-like and NADC34-like PRRSVs were the predominant strains on this pig farm. Importantly, NADC34-like PRRSV-detected during the period of peak mortality-was one of the predominant strains on this pig farm. Sequence alignment suggested that these strains shared the same 100 aa deletion in the NSP2 protein as IA/2014/NADC34 isolated from the United States (U.S.) in 2014. Phylogenetic analysis based on open reading frame 5 (ORF5) showed that the genetic diversity of NADC34-like PRRSV on this farm was relatively singular, but it had a relatively high rate of evolution. Restriction fragment length polymorphism (RFLP) pattern analysis showed that almost all ORF5 RFLPs were 1-7-4, with one 1-4-4. In addition, two complete genomes of NADC34-like PRRSVs were sequenced. Recombination analysis and sequence alignment demonstrated that both viruses, with 98.9% nucleotide similarity, were non-recombinant viruses. This study reports the prevalence and characteristics of NADC34-like PRRSVs on a large-scale breeding farm in northern China for the first time. These results will help to reveal the impact of NADC34-like PRRSVs on Chinese pig farms, and provide a reference for the detection and further prevention and control of NADC34-like PRRSVs.

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