数字农科院2.0

Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus)

文献类型： 外文期刊

作者： Zhu, Yingchun;Yuan, Gaopeng;Gao, Bowen;An, Guolin;Li, Weihua;Si, Wenjing;Sun, Dexi;Liu, Junpu

作者机构：

关键词： comparative transcriptome;salt tolerance;watermelon;molecular mechanism;TCP

期刊名称： LIFE-BASEL

ISSN：

年卷期： 2022 年 12 卷 7 期

页码：

摘要： Salt stress seriously reduced the yield and quality of watermelon and restricted the sustainable development of the watermelon industry. However, the molecular mechanism of watermelon in response to salt stress is still unclear. In this study, 150 mmol.L-1 NaCl was used to deal with the seedlings of salt-tolerant and salt-sensitive watermelon varieties. Physiological characteristics showed that salt stress significantly reduced the biomass of watermelon seedlings and the accumulation of K+ in roots and leaves and significantly increased the content of Na+, Cl-, and malondialdehyde (MDA). Compared with the salt-sensitive variety, the salt-tolerant variety had higher K+ accumulation, lower Cl-, Cl- accumulation, and MDA content in roots and leaves. Then, RNA-seq was performed on roots and leaves in normal culture and under 150 mmol.L-1 NaCl treatment. A total of 21,069 genes were identified by RNA-seq analysis, of which 1412 were genes encoding transcription factors (TFs). In the comparison groups of roots and leaves, 122 and 123 shared differentially expressed genes (DEGs) were obtained, respectively. Gene ontology (GO) annotation and KEGG enrichment results showed that there were many identical GO terms and KEGG pathways in roots and leaves, especially the pathways that related to sugar or energy (ATP or NADP+/NADPH). In addition, some DEGs related to salt tolerance were identified, such as plant hormone indole-3-acetic acid (IAA) and gibberellin (GA) signal transduction pathway-related genes, K+/Na+/Ca2+-related genes, lignin biosynthesis-related genes, etc. At the same time, we also identified some TFs related to salt tolerance, such as AP2-EREBP, bZIP, bHLH, MYB, NAC, OFP, TCP, and WRKY and found that these TFs had high correlation coefficients with salt tolerance-related genes, indicating that they might have a potential regulatory relationship. Interestingly, one TCP TF (Cla97C09G174040) co-exists both in roots and leaves, and it is speculated that it may be regulated by miR319 to improve the salt tolerance of watermelon.

分类号：

  • 相关文献

[1]Comparative Transcriptome Analysis Reveals Differential Gene Expression in Resistant and Susceptible Watermelon Varieties in Response to Meloidogyne incognita. Yingchun Zhu,Gaopeng Yuan,Renzong Zhao,Guolin An,Weihua Li,Wenjing Si,Junpu Liu,Dexi Sun. 2022

[2]Overexpression of Mtr-miR319a Contributes to Leaf Curl and Salt Stress Adaptation in Arabidopsis thaliana and Medicago truncatula. Mingna Li,Lei Xu,Lixia Zhang,Xiao Li,Chunyu Cao,Lin Chen,Junmei Kang,Qingchuan Yang,Yajiao Liu,Bilig Sod,Ruicai Long. 2022

[3]Transcriptome and Metabolome Analysis Reveals Salt-Tolerance Pathways in the Leaves and Roots of ZM-4 (Malus zumi) in the Early Stages of Salt Stress. Dajiang Wang,Kun Wang,Simiao Sun,Peng Yan,Xiang Lu,Zhao Liu,Qingshan Li,Lianwen Li,Yuan Gao,Jihong Liu. 2023

[4]Progress on Salt Tolerance in Brassica napus. Rui Dai,Na Zhan,Rudan Geng,Kun Xu,Xiangchun Zhou,Lixia Li,Guixin Yan,Fanglin Zhou,Guangqin Cai. 2024

[5]Transcriptomic and Metabolomic Analysis of the Effects of Exogenous Trehalose on Salt Tolerance in Watermelon (Citrullus lanatus). Yuan, Gaopeng,Sun, Dexi,An, Guolin,Li, Weihua,Si, Wenjing,Liu, Junpu,Zhu, Yingchun. 2022

[6]棉花TCP家族转录因子基因GhTCP1的克隆与表达分析. 刘伟娜,韩建民,董金皋,李学勇. 2010

[7]菜薹TCP家族基因鉴定及其对腋芽性状的影响. 朱彩烁,李建忠,张倩男,徐丽杰,张淑江,章时蕃,李菲,张慧,徐进,宋子涵,李国亮. 2025

[8]西瓜EST资源的SSR信息分析及标记开发. 赵胜杰,刘文革,阎志红,何楠,包文风. 2009

[9]施用钾肥对西瓜番茄红素含量的影响. 何楠,赵胜杰,刘文革,阎志红,万学闪. 2009

[10]NaCl胁迫对不同西瓜种质发芽的影响. 阎志红,刘文革,赵胜杰,何楠,王俊良. 2009

[11]不同倍性西瓜果实Vc含量比较研究. 程志强,刘文革,刘志敏,阎志红,赵胜杰,何楠,张俊杰. 2009

[12]不同染色体倍性西瓜花粉形态观察比较. 刘文革,王鸣,阎志红. 2009

[13]Leaching behavior of chlorpyrifos and its main metabolite TCP through 5 types of soil columns in laboratory conditions. Sun, Baoli,Shan, Hong,Dong, Yiwei,Huang, Jinli,Tong, Chengfeng. 2014

[14]Characterization of a barley (Hordeum vulgare L.) mutant with multiple stem nodes and spikes and dwarf (msnsd) and fine-mapping of its causal gene. Dandan D. Qin,Rui Liu,Fuchao Xu,Guoqing Dong,Qing Xu,Yanchun Peng,Le Xu,Hongna Cheng,Ganggang Guo,Jing Dong,Chengdao Li. 2023

[15]OsDA1 positively regulates grain width in rice. Li, Cong,Liu, Jun,Zhang, Liya,Li, Tao,Li, Hongyu,Liu, Bin,Zhao, Tao. 2024

[16]The trxG protein ULT1 regulates Arabidopsis organ size by interacting with TCP14/15 to antagonize the LIM peptidase DA1 for H3K4me3 on target genes. Xu, Fan,Dong, Huixue,Guo, Weijun,Le, Liang,Jing, Yexing,Fletcher, Jennifer C.,Sun, Jiaqiang,Pu, Li. 2024

[17]Stress-induced higher vein density in the C-3-C-4 intermediate Moricandia suffruticosa under drought and heat stress. Zhu, Xiaoyi,Liu, Jun,Sun, Xingchao,Kuang, Chen,Liu, Hongfang,Zhang, Liang,Zheng, Qiwei,Liu, Jing,Li, Jun,Wang, Hanzhong,Hua, Wei. 2022

[18]Comparative Transcriptome Analysis Reveals the Mechanisms Underlying Differences in Salt Tolerance Between indica and japonica Rice at Seedling Stage. Weilong Kong,Tong Sun,Chenhao Zhang,Xiaoxiao Deng,Yangsheng Li. 2021

[19]Comparative Transcriptome Analyses of the Developmental Stages of Taenia multiceps. Wen Hui Li,Yang Yang,Nian Zhang Zhang,Jian Kui Wang,Yin Ju Liu,Li Li,Hong Bin Yan,Wan Zhong Jia,Baoquan Fu. 2021

[20]Comparative Transcriptome Analyses of the Developmental Stages of Taenia multiceps. Wen Hui Li,Yang Yang,Nian Zhang Zhang,Jian Kui Wang,Yin Ju Liu,Li Li,Hong Bin Yan,Wan Zhong Jia,Baoquan Fu. 2021

作者其他论文 更多>>