数字农科院2.0

The characteristics of mRNA m6A methylomes in allopolyploid Brassica napus and its diploid progenitors

文献类型： 外文期刊

作者： Li, Zeyu;Li, Mengdi;Wu, Xiaoming;Wang, Jianbo

作者机构：

期刊名称： HORTICULTURE RESEARCH

ISSN： 2662-6810

年卷期： 2023 年 10 卷

页码：

收录情况： SCIE（2023版） ; ; CSCD（2023-2024年度） ; ; 科技核心（2023版） ; ; 农林核心（2020版）

摘要： Genome duplication events, comprising whole-genome duplication and single-gene duplication, produce a complex genomic context leading tomultiple levels of genetic changes. However, the characteristics of m(6)Amodification, themostwidespread internal eukaryotic mRNA modification, in polyploid species are still poorly understood. This study revealed the characteristics of m(6)A methylomes within the early formation and following the evolution of allopolyploid Brassica napus. We found a complex relationship between m(6)A modification abundance and gene expression level depending on the degree of enrichment or presence/absence of m(6)A modification. Overall, the m(6)A genes had lower gene expression levels than the non-m(6)A genes. Allopolyploidization may change the expression divergence of duplicated gene pairs with identical m(6)A patterns and diverged m(6)A patterns. Compared with duplicated genes, singletons with a higher evolutionary rate exhibited higher m(6)A modification. Five kinds of duplicated genes exhibited distinct distributions of m(6)A modifications in transcripts and gene expression level. In particular, tandem duplication-derived genes showed unique m6A modification enrichment around the transcript start site. Active histone modifications (H3K27ac and H3K4me3) but not DNA methylation were enriched around genes of m6A peaks. These findings provide a new understanding of the features of m (6)A modification and gene expression regulation in allopolyploid plants with sophisticated genomic architecture.

分类号：

  • 相关文献
作者其他论文 更多>>