数字农科院2.0

Broflanilide induces zebrafish neurobehavioral defects by interfering with synaptic homeostasis

文献类型： 外文期刊

作者： Kai Wang;Xiyan Mu;Xiaoyu Liu;Lu He;Chengju Wang

作者机构：

关键词： broflanilide;Neural behavior changes;Neural cell heterogeneous response;Synaptic injury;Zebrafish

期刊名称： Aquatic Toxicology

ISSN： 0166-445X

年卷期： 2025 年 283 卷

页码：

收录情况： SCIE（2025版）

摘要： Broflanilide is a novel bisamide insecticide that is extensively used. Previous study reported that broflanilide induced neurotoxicity during zebrafish embryonic development, however, its behavior impact and the involved neural cell heterogeneous mechanism remains largely unknown. Here, we performed a series of neuro-behavior test for adult zebrafish that were exposed to 5 and 25 μg/L broflanilide for 30 days. We found that 25 μg/L broflanilide could induce abnormal locomotion and cognitive defect. These changes were accompanied by synaptic homeostasis inference (decreased number of synaptic knobs), and neuron loss. Simultaneously, a targeted metabolomic assay showed that the glutathione metabolism and GABAergic synapses in brain were significantly altered, indicating an altered synaptic transmission, which is consistent with the synaptic injury. Single-cell RNA sequencing of zebrafish brain showed changed cell composition after exposure, including a decreased ratio of neuron and oligodendrocyte, and an increased proportion of astrocytes. Meanwhile, genes involved in synaptic functional pathways were altered in neuron, astrocyte and oligodendrocytes, which partly explained the disruption of synaptic homeostasis. These findings reveal the long-term risk of broflanilide toward neural health of aquatic organisms and suggest an across-cell types transcriptional regulation in mediating the neurotoxicity.

分类号：

  • 相关文献

[1]Simultaneous Determination Of Broflanilide And I.ts Metabolites In Five T ypical Chinese Soils By A Modified Quick, Easy, Cheap, Effective, Rugged, And Safe Method With Ultra High Performance Liquid Chromatography. Liu, Xingang,Wu, Xiaohu,An, Xiaokang,Dong, Fengshou,Xu, Jun,Wang, Ran,Zheng, Yongquan. 2018

[2]Toxic effects of broflanilide exposure on development of zebrafish (Danio rerio) embryos and its potential cardiotoxicity mechanism. Manman Duan,Jie Zhang,Jia Liu,Le Qian,Xiangguang Chen,Feng Zhao,Wentian Zhao,Zhaomin Zhong,Yang Yang,Chengju Wang. 2021

[3]Effect of broflanilide on the phytophagous mite Tetranychus urticae and the predatory mite Typhlodromips swirskii. Ning Shen,Yao Li,Kipchoge Leviticus,Xiao Li Chang,Tao Tang,Li Cui,Zhao Jun Han,Chun Qing Zhao. 2021

[4]Neural System Impairment and Involved Microglia-Neuron Regulation of Broflanilide in Zebrafish Larvae. Mu, Xiyan,Wang, Kai,He, Lu,Liu, Zaiteng,Zhang, Yining,Zhang, Ru,Duan, Manman,Wang, Chengju. 2023

[5]Effects of azocyclotin on gene transcription and steroid metabolome of hypothalamic-pituitary-gonad axis, and their consequences on reproduction in zebrafish (Danio rerio). Ma, You-Ning,Cao, Chu-Yan,Wang, Qiang-Wei,Gui, Wen-Jun,Zhu, Guo-Nian,Ma, You-Ning.

[6]The enantioselective toxicity and oxidative stress of beta-cypermethrin on zebrafish. Mu, Xiyan,Shen, Gongming,Huang, Ying,Luo, Jianbo,Li, Yingren,Zhu, Lizhen,Wang, Chengju,Li, Xuefeng,Qi, Suzhen.

[7]Coordinate involvement of Nodal-dependent inhibition and Wnt-dependent activation in the maintenance of organizer-specific bmp2b in zebrafish. Xue, Yu,Zhang, Wenjuan,Chen, Canbin,Xu, Jingjin,Pan, Yutian,Xing, Cencan,Xing, Cencan,Meng, Anming.

[8]Subcellular localization, expression patterns, SNPs and association analyses of the porcine HUMMLC2B gene. Wang, Huan L.,Wang, Heng,Zhu, Zheng M.,Wang, Chen F.,Zhu, Meng J.,Mo, De L.,Yang, Shu L.,Li, Kui.

[9]Chiral bioaccumulation behavior of tebuconazole in the zebrafish (Danio rerio). Liu, Na,Dong, Fengshou,Xu, Jun,Liu, Xingang,Zheng, Yangquan.

[10]Stereoselective induction by 2,2 ',3,4 ',6-pentachlorobiphenyl in adult zebrafish (Danio rerio): Implication of chirality in oxidative stress and bioaccumulation. Chai, Tingting,Cui, Feng,Mu, Xiyan,Yang, Yang,Qi, Suzhen,Zhu, Lizhen,Wang, Chengju,Chai, Tingting,Qiu, Jing.

[11]Chlorpyrifos is estrogenic and alters embryonic hatching, cell proliferation and apoptosis in zebrafish. Yu, Kaimin,Li, Guochao,Feng, Weimin,Liu, Lili,Zhang, Jiayu,Wu, Wei,Xu, Lei,Yan, Yanchun.

[12]Stereoselective Determination of Tebuconazole in Water and Zebrafish by Supercritical Fluid Chromatography Tandem Mass Spectrometry. Liu, Na,Dong, Fengshou,Xu, Jun,Liu, Xingang,Chen, Zenglong,Tao, Yan,Pan, Xinglu,Chen, XiXi,Zheng, Yongquan.

[13]The effect of stabilized culture of Lactobacillus rhamnosus GCC-3 on gut and liver health, and anti-viral immunity of zebrafish. Hui Liang,Yadong Xie,Ming Li,Jie Chen,Wenhao Zhou,Rui Xia,Qianwen Ding,Yuanyuan Yao,Zhen Zhang,Yalin Yang,Chao Ran,Zhigang Zhou. 2023

[14]Toxic Effects Of Boscalid In A.dult Zebrafish (Danio Rerio) O n Carbohydrate And Lipid Metabolism. Qian, L, Zhang, J, Chen, XG, Qi, SZ, Wu, PZ, Wang, C, Wang, CJ. 2019

[15]Novel And Rapid Osteoporosis Model E.stablished In Zebrafish Using H igh Iron Stress. Zhang, WJ, Xu, JJ, Qiu, JH, Xing, CC, Li, XM, Leng, B, Su, Y, Lin, JM, Lin, JF, Mei, XQ, Huang, YQ, Pan, YT, Xue, Y. 2018

[16]The Enantioselective Toxicity And Oxidative S.tress Of Beta-Cypermethrin On Z ebrafish. Mu, XY, Shen, GM, Huang, Y, Luo, JB, Zhu, LZ, Qi, SZ, Li, YR, Wang, CJ, Li, XF. 2017

[17]Short-term exposure of Cannabidiol on Zebrafish (Danio Rerio): Reproductive Toxicity. Li, Lin,Fan, Bei,Kong, Zhiqiang,Zhang, Yifan,Zhao, Mengying,Simal-Gandara, Jesus,Wang, Fengzhong,Li, Minmin. 2023

[18]The role of miRNA-26a-5p and target gene socs1a in flutolanil induced hepatotoxicity of zebrafish at environmental relevant levels. Yang Yang,Xiyan Xu,Bin He,Jinhe Chang,Yongquan Zheng,Yuanbo Li. 2023

[19]Microplastics reduce the bioaccumulation and oxidative stress damage of triazole fungicides in fish. Changsheng Li,Shankui Yuan,Yanming Zhou,Xuefeng Li,Liusheng Duan,Lan Huang,周晓今,Yongqiang Ma,Sen Pang. 2022

[20]Lactobacillus plantarum WCFS1 alleviates Aeromonas hydrophila NJ-1-induced inflammation and muscle loss in zebrafish (Danio rerio). Senlin Li,Lina Guo,Xuemeng Si,Zhaolai Dai,Zhigang Zhou,Zhenlong Wu. 2022

作者其他论文 更多>>