数字农科院2.0

Identification of miRNAs Involved in Olfactory Regulation in Antennae of Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae)

文献类型： 外文期刊

作者： Yu Zhang;Yanyan Li;Haibin Han;Xiaoling Wang;Shujing Gao;Qing Zhao;Halima Bieerdebieke;Linbo Xu;Qicong Zang;Hui Wang;Penghua Bai;Kejian Lin

作者机构：

关键词： expression levels;Loxostege sticticalis;microRNAs;olfactory-related genes;target prediction

期刊名称： Life

ISSN： 2075-1729

年卷期： 2024 年 14 卷 12 期

页码：

收录情况： SCIE（2024版）

摘要： The beet webworm, Loxostege sticticalis, is a typical migratory pest. Although miRNAs participate in many physiological functions, little is known about the functions of miRNAs in olfactory regulation. In this study, 1120 (869 known and 251 novel) miRNAs were identified in the antennae of L. sticticalis by using high-throughput sequencing technology. Among the known miRNAs, 189 from 49 families were insect-specific, indicating that these miRNAs might play unique roles in insects. Furthermore, based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we found that 3647 and 1393 miRNAs were associated with localization and the regulation of localization, respectively, and 80 miRNAs were enriched in the neuroactive ligand–receptor interaction pathway. These miRNAs might be involved in the olfactory system of L. sticticalis. Notably, qRT-PCR showed that most of the tested miRNAs presented similar expression patterns compared with the RNA-seq data and that miR-87-3, novel-miR-78, and novel-miR-142 were significantly differentially expressed in the antennae of males and females. In addition, 21 miRNAs were predicted to target 23 olfactory genes, including 10 odorant-binding proteins (OBPs), 3 chemosensory proteins (CSPs), 4 odorant receptors (ORs), 1 ionotropic receptor (IR), and 5 gustatory receptors (GRs). The olfactory-related miRNAs exhibited low-abundance transcripts, except undef-miR-55 and undef-miR-523, and gender-biased expression was not observed for olfactory-related miRNAs. Our findings provide an overview of the potential miRNAs involved in olfactory regulation, which may provide important information on the function of miRNAs in the insect olfactory system.

分类号：

  • 相关文献

[1]Genome-wide identification of PME gene family and expression of candidate genes associated with aluminum tolerance in tea plant (Camellia sinensis). Huang, Danjuan,Mao, Yingxin,Guo, Guiyi,Ni, Dejiang,Chen, Liang. 2022

[2]感染日本血吸虫小鼠循环血miRNAs变化分析. 朱丽慧,刀金威,杜晓利,李浩,陆珂,刘金明,程国锋. 2015

[3]microRNAs在植物发育调控中的功能研究进展. 王迪,傅彬英,黎志康,张立军. 2006

[4]MicroRNAs 与脂类代谢. 南雪梅,王加启,陈海燕,胡菡. 2013

[5]MicroRNAs与脂类代谢. 南雪梅,王加启,陈海燕,胡菡. 2013

[6]顶复门原虫诱导宿主microRNAs变化的研究进展. 范艺凡,周东辉,徐民俊,李成琳,朱兴全,蒋文灿. 2012

[7]MicroRNA通路在多房棘球蚴多功能干细胞neoblasts中的调控作用. 郭小腊,郑亚东. 2015

[8]弓形虫感染食蟹猴的实验研究. 李海龙,袁子国,张秀香,宋慧群,周东辉,徐民俊,林瑞庆,宁章勇,黄思扬,朱兴全. 2011

[9]油菜和拟南芥菌核病抗性相关基因的功能研究. 任秋红,黄军艳,田保明,刘胜毅. 2009

[10]Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development. Yang Ya-lan,Li Yan,Liang Ru-yi,Zhou Rong,Ao Hong,Mu Yu-lian,Yang Shu-lin,Li Kui,Tang Zhong-lin,Li Yan. 2014

[11]The microRNAs in the antennae of Apolygus lucorum (Hemiptera: Miridae): Expression properties and targets prediction. Khashaveh A.,An X.,Shan S.,Pang X.,Li Y.,Fu X.,Zhang Y.. 2022

[12]Egg Hatch and Survival and Development of Beet Webworm (Lepidoptera: Crambidae) Larvae at Different Combinations of Temperature and Relative Humidity. Tang, Jihong,Cheng, Yunxia,Jiang, Xingfu,Zhang, Lei,Luo, Lizhi,Sappington, Thomas W..

[13]Three amino acid residues of an odorant-binding protein are involved in binding odours in Loxostege sticticalis L.. Zhuang, X.,Wang, Q.,Cao, Y.,Zhang, S.,Li, K.,Xiao, C..

[14]Complete mitochondrial genome of the meadow moth, Loxostege sticticalis (Lepidoptera: Pyraloidea: Crambidae), compared to other Pyraloidea moths. Zheng, Xi-Xi,Peng, Ming-Hui,Bian, Hai-Xu,Chen, Miao-Miao,Liu, Yan-Qun,Qin, Li,Jiang, Xing-Fu. 2016

[15]JUVENILE HORMONE CHANGES ASSOCIATED WITH DIAPAUSE INDUCTION, MAINTENANCE, AND TERMINATION IN THE BEET WEBWORM, Loxostege sticticalis (LEPIDOPTERA: PYRALIDAE). Jiang, Xingfu,Huang, Shaohong,Luo, Lizhi. 2011

[16]Molecular characters and recombinant expression of the carboxylesterase gene of the meadow moth Loxostege sticticalis L. (Lepidoptera: Pyralidae). Zhong, Tao,Li, Ke-Bin,Cao, Ya-Zhong,Guo, Wei,Wei, Zhao-Jun. 2011

[17]Response of Reproductive Traits and Longevity of Beet Webworm to Temperature, and Implications for Migration. Wang, Kai,Luo, Lizhi,Jiang, Xingfu,Sappington, Thomas W.. 2015

[18]Density-dependent prophylaxis in crowded Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae) larvae to a parasitoid and a fungal pathogen. Kong, Hailong,Cheng, Yunxia,Luo, Lizhi,Jiang, Xingfu,Zhang, Lei,Sappington, Thomas W.. 2013

[19]Comparison of Reproductive and Flight Capacity of Loxostege sticticalis (Lepidoptera: Pyralidae), Developing From Diapause and Non-Diapause Larvae. Luo, Lizhi,Jiang, Xingfu,Zhang, Lei,Xie, Daosong,Sappington, Thomas W.. 2012

[20]Identification and Molecular Characterization of a Chitin-Binding Protein from the Beet Webworm, Loxostege sticticalis L.. Yang, Shuang,Li, Kebin,Cao, Yazhong,Guo, Wei. 2014

作者其他论文 更多>>