数字农科院2.0

The deletion of the EP402R and MGF505/360 genes attenuates a genotype I/II recombinant ASFV but fails to confer complete protection against homologous or genotype II challenge in pigs

文献类型： 外文期刊

作者： Yao Li;Yingnan Liu;Zhuyun Sun;Zhenhua Xie;Chuanwen Tian;Rongrong Wang;Jun Gao;Maomao Wang;Jingyi Liu;Heng Wang;Guihong Zhang;Jie Li;Dongdong Di;Lang Gong;Hongjun Chen

作者机构：

关键词： African swine fever virus;cross-protection;EP402R;gene deletion;genotype I and II

期刊名称： Emerging Microbes and Infections

ISSN：

年卷期： 2026 年 15 卷 1 期

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收录情况： SCIE（2025版）

摘要： African swine fever (ASF), a pig disease caused by ASFV, is highly contagious and often lethal. The recent emergence of novel ASFV with I/II genomic recombination has posed significant challenges to global ASF prevention and control. In this study, we used the Chinese I/II genomic recombinant virulent strain ASFV-HN10005 (ASFV-HN) to construct two gene-deleted viruses. ASFV-HNΔMGF lacks the MGF505-1R-MGF360-14L genes cluster (including MGF505-1R, -2R, -3R, and MGF360-12L, -13L, -14L), and ASFV-HNΔCD2vΔMGF lacks both EP402R and the MGF505-1R-MGF360-14L genes cluster. ASFV-HNΔMGF still showed pathogenicity in domestic pigs, while ASFV-HNΔCD2vΔMGF showed markedly attenuated virulence, with all inoculated pigs surviving. However, these pigs did not gain complete protection against subsequent lethal challenge from the parental ASFV-HN strain or the virulent II-type strain ASFV-GZ. This implies that I/II genomic recombinant ASFV may have unique biological traits and immune evasion mechanisms. Our findings indicate the need to reassess current ASFV control strategies and provide a basis for future vaccine target selection.

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