GmSHMT08-Mediated Soybean Cyst Nematode Resistance Is Negatively Modulated by Two Heat Shock Protein 70s in Soybean
文献类型: 外文期刊
作者: Zhang, Liuping;Xiang, Chao;Zheng, Na;Kang, Houxiang;Kong, Lingan;Huang, Wenkun;Peng, Huan;Lian, Yun;Lu, Weiguo;Gao, Mingjie;Wang, Jiajun;Meksem, Khalid;Peng, Deliang;Yu, Feng;Liu, Shiming
作者机构:
关键词: GmHSP70-13a;GmHSP70-15a;GmSHMT08;resistance;SCN;soybean
期刊名称: PLANT CELL AND ENVIRONMENT
ISSN: 0140-7791
年卷期: 2025 年
页码:
收录情况: SCIE(2025版)
摘要: The soybean GmSHMT08 (Rhg4) is a major gene conferring resistance to soybean cyst nematode (SCN), which is a devastating pathogen in soybean, yet its underlying resistance mechanism remains elusive. Heat shock protein 70 (HSP70) mainly functions in maintaining protein homoeostasis and regulating plant immunity. In this study, CRISPR/Cas9-mediated knockout of two highly similar GmHSP70s [GmHSP70-13a (Glyma.13g254900) and GmHSP70-15a (Glyma.15g059900)] enhanced, whereas overexpressing either of them suppressed, SCN resistance. Both GmHSP70-13a and GmHSP70-15a interacted with the key one-carbon metabolism enzyme GmSHMT08, and promoted GmSHMT08 degradation via 26S proteasome pathway, but neither of them altered GmSHMT08 transcript levels in soybean. Furthermore, both GmHSP70-13a and GmHSP70-15a were strongly induced in susceptible soybeans, while both of them still remained low in resistant soybeans, after SCN infection. Taken together, GmSHMT08-mediated SCN resistance is shown to be negatively modulated by both GmHSP70-13a and GmHSP70-15a. The regulation is facilitated through interacting with and influencing 26S proteasome system-involved degradation of GmSHMT08. This study elucidates a SCN-resistance mechanism of GmSHMT08, expands functions of HSP70s and provides two GmHSP70s gene-editing targets for soybean SCN resistance breeding.
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