数字农科院2.0

A real-time visualized TRSV-based gene silencing method using trichome as a selected marker in cucumber

文献类型： 外文期刊

作者： Xu Wang;Chenhao Zhou;Xiaoli Gao;Jia Luo;Yong He;Lihao Wang;Zhi Xu;Zhujun Zhu;Yunmin Xu

作者机构：

关键词： CsGL3;Cucumber;Real-time visualization;Trichome;TRSV;VIGS

期刊名称： Plant Science

ISSN： 0168-9452

年卷期： 2025 年 360 卷

页码：

收录情况： SCIE（2025版）

摘要： Cucumber (Cucumis sativus. L) is economically valuable vegetable crop worldwide. Although cucumber genomic sequence has been completed, the functions of most genes have not yet been characterized. Virus-induced gene silencing (VIGS) is an efficient system for investigating gene function in plants, however, detection of VIGS efficiency by PCR is a time-consuming method. In this study, a vacuum-agroinfiltrated Tobacco ringspot virus (TRSV)-based gene silencing method was developed in cucumber, and CsGLABROUS3 (CsGL3), which functions in initiation of trichome, was cloned into pTRSV2 vector to develop a TRSV-CsGL3 system. Gene silenced cucumbers were visible using trichome as a selected marker, and their glabrous phenotype exhibited throughout the life cycle in TRSV-CsGL3 system. Thereafter, a flower morphogenesis gene (UNUSUAL FLORAL ORGANS, CsUFO) was selected to silence by the TRSV-CsGL3 system, and CsUFO silenced cucumbers produced the flower defect phenotype as expected. In summary, TRSV-CsGL3 is a real-time visualized VIGS system using trichome as a selected marker, which simplify the VIGS identification procedure, and it can be used to investigate gene function throughout the life cycle in cucumber.

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