数字农科院2.0

DMP1-Mediated FAK Activation Contributes to P Utilization of Broiler Osteoblasts by Suppressing FGF23 Expression

文献类型： 外文期刊

作者： Tingting Li;Xinyu Feng;Weiyun Zhang;Jingyi Zhao;Liyang Zhang;Yun Hu;Xiaoyan Cui;Shengchen Wang;Xugang Luo

作者机构：

关键词： broiler;DMP1;FAK;FGF23;P utilization;tibia osteoblasts

期刊名称： Biology

ISSN：

年卷期： 2026 年 15 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： Improving phosphorus (P) utilization in broilers is crucial for reducing feed costs and environmental pollution. Bone mineralization trait is strongly associated with P utilization in poultry and is thus often used as an alternative trait for evaluating P utilization. Dentin matrix protein 1 (DMP1), an essential matrix protein for bone mineralization and P deposition, has been shown to be actively involved in P utilization in broilers, but the underlying mechanisms remain unclear. The current study aimed to investigate the possible mechanisms whereby DMP1 regulates P utilization of poultry by using gene silencing and overexpression technologies, combined with an in vitro model of primary broiler osteoblasts. The results showed that DMP1 overexpression augmented the P utilization of broiler osteoblasts, characterized by significant increases (p < 0.001) in P utilization rate, mineralization formation, alkaline phosphatase activity, and bone gla protein content. Meanwhile, DMP1 overexpression effectively (p < 0.05) activated the focal adhesion kinase (FAK) signaling, along with obvious (p < 0.01) decreases in fibroblast growth factor 23 (FGF23) expression and production. In contrast, DMP1 silencing reversed (p < 0.05) the above effects. Consistently, FAK activation promoted (p < 0.05) P utilization accompanied by remarkable (p < 0.05) decreases in FGF23 expression and production. Furthermore, gain- and loss-of-function assays demonstrated that a high level of FGF23 contributed to impaired P utilization, while a low level was beneficial. Interestingly, blocking FAK signaling not only recovered (p < 0.05) the FGF23 expression and production in DMP1 overexpressed cells but also obviously (p < 0.05) weakened their P utilization. These findings indicate that DMP1 inhibits FGF23 expression by activating FAK, thereby contributing to P utilization in broiler osteoblasts. They reveal a novel DMP1-FAK-FGF23 regulatory axis in broiler osteoblasts and provide a potential target for improving P efficiency in poultry.

分类号：

  • 相关文献

[1]Dentin Matrix Protein 1 Silencing Inhibits Phosphorus Utilization in Primary Cultured Tibial Osteoblasts of Broiler Chicks. Li, Tingting,Geng, Yanqiang,Hu, Yun,Zhang, Liyang,Cui, Xiaoyan,Zhang, Weiyun,Gao, Feiyu,Liu, Zongping,Luo, Xugang. 2022

[2]The Effect of Bone Morphogenetic Protein 2 or Extracellular Signal-Regulated Kinase 1 Silencing on Phosphorus Utilization and Related Parameters in Primary Broiler Osteoblasts. Yanqiang Geng,Tingting Li,Yun Hu,Liyang Zhang,Xiaoyan Cui,Ling Zhu,Bingxin Wu,Xugang Luo. 2022

[3]Effects Of Clostridium Butyricum On G.rowth Performance, Lipid Metabolism A nd The Caecal Microecological Environment Of Broilers. Shi, S. R.,Ding, Xiao,Yang, Z. B.,Zhao, Xu,Shao, Dan,Shen, Yiru,Shi, S. R.. 2017

[4]Effects of two different broiler flooring systems on production performances, welfare, and environment under commercial production conditions. Alphin, R.,Wen, X.,Zhu, Z.,Zhou, Z..

[5]Antioxidant capacity and meat quality of broilers exposed to different ambient humidity and ammonia concentrations. Wei, F. X.,Liu, F. Z.,Wei, F. X.,Li, S. Y.,Sun, Q. Y.,Hu, X. F.,Sa, R. N.. 2014

[6]Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers: 1. Intestinal structure and digestive function. Hao, Y.,Gu, X. H.,Wang, X. L.. 2012

[7]Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Gao, J.,Zhang, H. J.,Wu, S. G.,Qi, G. H.,Yu, S. H.,Gao, Y. P.,Yoon, I.,Quigley, J.. 2008

[8]An optimal dietary zinc level of broiler chicks fed a corn-soybean meal diet. Huang, Y. L.,Lu, L.,Luo, X. G.,Liu, B.. 2007

[9]Estimation of standardized phosphorus retention for corn, soybean meal, and corn-soybean meal diet in broilers. Liu, S. B.,Li, S. F.,Lu, L.,Xie, J. J.,Zhang, L. Y.,Luo, X. G.,Li, S. F.. 2012

[10]Effect of manganese source on manganese absorption by the intestine of broilers. Ji, F.,Luo, X. G.,Lu, L.,Liu, B.,Yu, S. X.. 2006

[11]Comparative ileal digestibility of amino acids in 00-rapeseed meal and rapeseed meal fed to growing male broilers. Ullah, Zafar,Sarwar, Muhammad,Rehman, Zaib Ur,Yin, Yulong,Stein, Hans H.,Ullah, Zafar,Hayat, Zafar,Ahmed, Gulraiz,Nisa, Mahr Un,Akhtar, Muhammad,Rehman, Zaib Ur.

[12]Bioavailability of organic manganese sources in broilers fed high dietary calcium. Li, SF,Luo, XG,Lu, L,Crenshaw, TD,Bu, YQ,Liu, B,Kuang, X,Shao, GZ,Yu, SX. 2005

[13]Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Song, J.,Xiao, K.,Ke, Y. L.,Jiao, L. F.,Hu, C. H.,Zou, X. T.,Diao, Q. Y.,Shi, B.. 2014

[14]The influences of ambient temperature and crude protein levels on performance and serum biochemical parameters in broilers. Liu, Q. W.,Chao, Z.,Wei, L. M.,Wang, F.,Sun, R. P.,Feng, J. H.,Chen, Y.,Zhang, M. H..

[15]Cholesterol metabolism changes in the early stages of ascites in broiler chickens. Zhang, Shan,Zhao, Xu,Shen, Yiru,Shi, S. R.,Tong, H. B.,Guo, Y. M.,Shi, S. R..

[16]Changes in liver triglyceride metabolism in broiler chickens with cold-induced ascites syndrome. Zhao, Xu,Zhang, Shan,Shen, Yiru,Shi, S. R.,Guo, Y. M.,Shi, S. R..

[17]Ammonia concentration and relative humidity in poultry houses affect the immune response of broilers. Wei, F. X.,Zhang, M. H.,Wei, F. X.,Xu, B.,Li, S. Y.,Sun, Q. Y.,Lin, P.,Hu, X. F.. 2015

[18]Effect of beta-hydroxy-beta-methylbutyrate calcium on growth, blood parameters, and carcass qualities of broiler chickens. Qiao, X.,Zhang, H. J.,Wu, S. G.,Yue, H. Y.,Qi, G. H.,Qiao, X.,Zuo, J. J.,Feng, D. Y.,Qiao, X.,Zhang, H. J.. 2013

[19]Effects of a thermostable phytase on the growth performance and bone mineralization of broilers. Tang, H. O.,Gao, X. H.,Ji, F.,Tong, S.,Li, X. J.. 2012

[20]Effect of Intravenously Injected Manganese on the Gene Expression of Manganese-Containing Superoxide Dismutase in Broilers. Li, S. F.,Luo, X. G.,Lu, L.,Liu, B.,Kuang, X.,Shao, G. Z.,Yu, S. X.,Li, S. F.,Luo, X. G.,Lu, L.,Liu, B.,Shao, G. Z.,Yu, S. X.,Li, S. F.. 2008

作者其他论文 更多>>