数字农科院2.0

Dihydrolipoyl Dehydrogenase from Mycoplasma capricolum subsp. capripneumoniae: An Immunogenic Plasminogen-Binding Putative Adhesin

文献类型： 外文期刊

作者： Ziqing Wang;Jiazhen Ge;Guodong Song;Yijian Liu;Qianqian Li;Renge Li;Pengcheng Gao;Fuying Zheng;Yuefeng Chu

作者机构：

关键词： Adhesin Dihydrolipoyl dehydrogenase;Capripneumoniae;Mycoplasma capricolum;Plasminogen;Subcellular localization;subsp

期刊名称： Pakistan Veterinary Journal

ISSN： 2074-7764

年卷期： 2025 年 45 卷 3 期

页码：

收录情况： SCIE（2025版）

摘要： Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum ubsp. capripneumoniae (Mccp), is a severe respiratory disease affecting global goat arming. Mccp infection relies on its adhesion to the host respiratory tract. Dihydrolipoamide dehydrogenase (DLD) is a flavoprotein oxidoreductase that forms disulfide bonds using flavin adenine dinucleotide (FAD). It typically exists as a homodimer, with each 51 kDa subunit covalently linked to one FAD molecule. Although DLD's structure and enzymatic function are well understood, its role in Mccp pathogenicity remains unclear. In our study, rDLD (rMccpDLD) was expressed n Escherichia coli BL21(DE3) pLysS and subsequently purified from the upernatant, and mouse antibodies confirmed its presence on the Mccp cell surface. ndirect immunofluorescence showed rMccpDLD binds to GTEC epithelial cells, ndicating its role in adhesion. Mouse anti-rMccpDLD serum significantly inhibited M1601 strain adhesion to these cells. Additionally, DLD interacts with host plasminogen, with molecular dynamics simulations confirming stable binding. These esults suggest DLD acts as both a virulence factor and adhesin during Mccp nfection, making it a potential target for developing new CCPP treatments and vaccines.

分类号：

  • 相关文献

[1]东方蜜蜂在我国的分布及其亚种分化. 杨冠煌. 1990

[2]土壤湿度对Acidovoraxavenaesubsp.citrulli从种子表面传播到哈密瓜幼苗上的影响. 王建荣,孙福在,回文广,谢扬军. 2003

[3]河南西瓜甜瓜发生细菌性果腐病. 古勤生,徐永阳,彭斌,徐志红,刘丽锋,李莉. 2004

[4]河南省西瓜甜瓜细菌性果腐病病原细菌的分离与鉴定. 古勤生,李鹏,郑文杰,彭斌,唐乐尘,杨渡,NormanW．Schaad. 2006

[5]关于硅酸盐细菌分类研究的历史及现状. 徐玲玫. 2001

[6]哈密瓜果斑病菌血清学快速检测. 赵廷昌,孙福在,王建荣. 2004

[7]Molecular characterization of enolase gene from Taenia multiceps. Li, W. H.,Qu, Z. G.,Zhang, N. Z.,Yue, L.,Jia, W. Z.,Luo, J. X.,Yin, H.,Fu, B. Q.,Jia, W. Z.,Luo, J. X.,Yin, H.,Fu, B. Q..

[8]Mycoplasma synoviae dihydrolipoamide dehydrogenase is an immunogenic fibronectin/plasminogen binding protein and a putative adhesin. Jingjing Qi,Yu Wang,Haoran Li,Yuanbing Shang,Song Gao,Chan Ding,Xiaohan Liu,Shaohui Wang,Tao Li,Mingxing Tian,Shengqing Yu. 2022

[9]LppA is a novel plasminogen receptor of Mycoplasma bovis that contributes to adhesion by binding the host extracellular matrix and Annexin A2. Shuang Liu,Zhangcheng Li,Shimei Lan,Huafang Hao,Xiangrui Jin,Jinjia Liang,Ahmed Adel Baz,Xinmin Yan,Pengcheng Gao,Shengli Chen,Yuefeng Chu. 2023

[10]Mesomycoplasma (Mycoplasma) ovipneumoniae dihydrolipoamide dehydrogenase is an immunogenic plasminogen binding protein and a putative adhesin. Jiazhen Ge,Tongtong Tian,Yijian Liu,Xuerui Li,Qianqian Li,Guodong Song,Pengcheng Gao,Fuying Zheng,Yuefeng Chu. 2024

[11]The chaperone GrpE mediates adhesion in Mycoplasma bovis and interactions with host extracellular matrix components and plasminogen. Jin, Xiangrui,Hao, Huafang,Baz, Ahmed Adel,Jin, Shanyu,Lan, Shimei,Li, Zhangcheng,Zhang, Yifan,Chu, Yuefeng,Chen, Shengli. 2025

[12]FtsZ contributes to cytoadhesion and interaction with host extracellular matrix components and plasminogen in Mycoplasma bovis. Shanyu Jin,Shengli Chen,Huafang Hao,Shimei Lan,Xiangrui Jin,Zhangcheng Li,Xinyi Wang,Yifan Zhang,Xinmin Yan,Yuefeng Chu. 2025

[13]Importance of the rice TCD9 encoding alpha subunit of chaperonin protein 60 (Cpn60 alpha) for the chloroplast development during the early leaf stage. Jiang, Quan,Mei, Jie,Gong, Xiao-Di,Lin, Dong-Zhi,Dong, Yan-Jun,Xu, Jian-Long,Zhang, Jian-Hui,Teng, Sheng. 2014

[14]Long Chain Acyl-Coenzyme A Synthetase 4 (BnLACS4) Gene from Brassica napus Enhances the Yeast Lipid Contents. Tan Xiao-li,Zheng Xiang-feng,Zhang Zhi-yan,Wang Zheng,Xia Heng-chuan,Gu Shou-lai,Lu Chang-ming. 2014

[15]Cloning and analysis of the soybean MEKK gene. Sha, A. -H.,Ba, H. -P.,Shan, Z. -H.,Chen, H. -F.,Chen, S. -L.,Qiu, D. -Z.,Zhou, X. -A.,Sha, A. -H.,Chen, Y. -H.. 2015

[16]An alfalfa (Medicago sativa L.) ethylene response factor gene, MsERF11, enhances salt tolerance in transgenic Arabidopsis. Chen, Tingting,Yang, Qingchuan,Ding, Wang,Chen, Tingting,Zhang, Xinquan,Gruber, Margaret. 2012

[17]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[18]The rice TCD11 encoding plastid ribosomal protein S6 is essential for chloroplast development at low temperature. Wang, Wen-Juan,Zheng, Kai-Lun,Gong, Xiao-Di,Lin, Dong-Zhi,Dong, Yan-Jun,Gong, Xiao-Di,Xu, Jian-Long,Xu, Jian-Long,Huang, Ji-Rong.

[19]Molecular cloning and characterization of a novel gene encoding zinc finger protein from Medicago sativa L.. Chao, Yuehui,Kang, Junmei,Yang, Qingchuan,Wu, Mingsheng,Li, Yan,Chao, Yuehui,Wang, Pingqing,Long, Ruicai,Qin, Zhihui,Sun, Yan. 2009

[20]Stability and localization of 14-3-3 proteins are involved in salt tolerance in Arabidopsis. Tan, Tinghong,Cai, Jingqing,Zhan, Erbao,Zhou, Huapeng,Tan, Tinghong,Cai, Jingqing,Zhan, Erbao,Zhou, Huapeng,Yang, Yongqing,Guo, Yan,Zhao, Jinfeng.

作者其他论文 更多>>