数字农科院2.0

Combining manipulation of integration loci and secretory pathway on expression of an Aspergillus niger glucose oxidase gene in Trichoderma reesei

文献类型： 外文期刊

作者： Wangli Ji;Xiaolu Wang;Xiaoqing Liu;Yuan Wang;Fangui Liu;Bo Xu;Huiying Luo;Tao Tu;Wei Zhang;Xinxin Xu;Xiaoyun Su

作者机构：

关键词： Aspergillus niger;Glucose oxidase;Integration loci;Secretory pathway;Trichoderma reesei

期刊名称： Microbial cell factories

ISSN： 1475-2859

年卷期： 2023 年 22 卷 1 期

页码：

收录情况： SCIE（2023版）

摘要： Trichoderma reesei (T. reesei) is well-known for its excellent ability to secret a large quantity of cellulase. However, unlike the endogenous proteins, little is known about the molecular mechanisms governing heterologous protein production. Herein, we focused on the integration loci and the secretory pathway, and investigated their combinatorial effects on heterologous gene expression in T. reesei using a glucose oxidase from Aspergillus niger as a model protein. Integration in the cel3c locus was more efficient than the cbh1 locus in expressing the AnGOx by increasing the transcription of AnGOx in the early stage. In addition, we discovered that interruption of the cel3c locus has an additional effect by increasing the expression of the secretory pathway component genes. Accordingly, overexpressing three secretory pathway component genes, that were snc1, sso2, and rho3, increased AnGOx expression in the cbh1 transformant but not in the cel3c transformant.

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