数字农科院2.0

The Temporal Extracellular Proteomics Analysis Reveals the Expression Patterns of Functional Enzymes Involved in Ramie Degumming by Dickeya dadantii Strain DCE-01

文献类型： 外文期刊

作者： Yuqin Hu;Mingqiang Gao;Xiang Zhou;Lifeng Cheng;Guoguo Xi;Si Tan;Wei Zhou;Zishu Chen;Zhenghong Peng;An Wang;Shengwen Duan;Qi Yang

作者机构：

关键词： Dickeya dadantii strain DCE-01;differential protein expression;microbial degumming of ramie;proteomics

期刊名称： Polymers

ISSN：

年卷期： 2025 年 17 卷 24 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Microbial degumming offers an environmentally sustainable route for the extraction of natural ramie fibers. However, there are currently no genetically engineered bacteria suitable for large-scale industrial production. In this study, Dickeya dadantii strain DCE-01, a dominant ramie-degumming bacterium, was systematically investigated using time-resolved quantitative proteomics. This research revealed a temporally coordinated, multi-enzyme catalytic mechanism, in which pectate lyases were highly expressed at the initial stage to rapidly depolymerize surface pectin, followed by sustained expression of hemicellulases that ensured the complete removal of residual non-cellulosic materials. Quantitative real-time PCR (qRT–PCR) results showed strong concordance with the proteomic data, confirming that transcriptional regulation drives the dynamic enzymatic response. This integrative analysis, linking macroscopic morphological evolution with microscopic molecular changes, elucidates the intrinsic mechanism underlying efficient biological degumming by D. dadantii strain DCE-01 and provides valuable insights for the rational design of high-performance engineered bacteria for industrial fiber processing.

分类号：

  • 相关文献

[1]Two-Component Response Regulators CitT, YvcP, and YycI Differentially Control Pectin and Hemicellulose Degradation in Degumming of Ramie Fibers by Bacillus subtilis Strain 168. Qi Yang,Shihang Ma,Lifeng Cheng,Xiang Zhou,Guoguo Xi,Chen Chen,Zhenghong Peng,Yuqin Hu,Si Tan,Shengwen Duan. 2025

[2]Comparative proteomics analysis of pomegranate seeds on fruit maturation period (Punica granatum L.). CAO Shang-yin,Niu Juan,Cao Da,Li Haoxian,Xue Hui,Chen Lina,Zhang Fuhong,ZHAO Di-guang. 2015

[3]PROTEOMIC ANALYSIS OF NOVEL Cry1Ac BINDING PROTEINS IN Helicoverpa armigera (HUBNER). Chen, Li-Zhen,Liang, Ge-Mei,Zhang, Jie,Wu, Kong-Ming,Guo, Yu-Yuan,Rector, Brian G.. 2010

[4]Comparative Proteomic Analysis Shows an Elevation of Mdh1 Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats. Dong Shu-wei,Gao Zhao-hui,Li Xia,Shen Xiao-yun,Gao Zhao-hui,Xue Hui-wen. 2014

[5]Proteomics analysis of Xenopus laevis gonad tissue following chronic exposure to atrazine. Chen, Xiuping,Wang, Jiamei,Zhu, Haojun,Peng, Yufa,Wang, Jiamei,Zhu, Haojun,Ding, Jiatong. 2015

[6]Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains. Zhang, C. X.,Zhao, X.,Han, F.,Yang, M. F.,Chen, H.,Shen, S. H.,Zhang, C. X.,Chida, T.. 2009

[7]Metabolic responses and "omics" technologies for elucidating the effects of heat stress in dairy cows. Min, Li,Zhao, Shengguo,Tian, He,Zhou, Xu,Zhang, Yangdong,Li, Songli,Zheng, Nan,Wang, Jiaqi,Min, Li,Yang, Hongjian.

[8]Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis. Xue, Junxin,Xiao, Yan,Huang, Kehe,Xue, Junxin,Jiang, Wei,Chen, Yongjun,Liu, Yingchun,Zhang, Huajing,Wang, Quan,Qiao, Yuanbiao. 2016

[9]A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat. He, Xue,Fang, Jingjing,Li, Jingjuan,Qu, Baoyuan,Ren, Yongzhe,Ma, Wenying,Zhao, Xueqiang,Li, Bin,Wang, Daowen,Li, Zhensheng,Tong, Yiping,Fang, Jingjing,Li, Jingjuan,Ren, Yongzhe. 2014

[10]iTRAQ-based quantitative proteomic analysis of longissimus muscle from growing pigs with dietary supplementation of non-starch polysaccharide enzymes. Zhang, Ji-ze,Gao, Yang,Lu, Qing-ping,Sa, Ren-na,Zhang, Hong-fu,Gao, Yang. 2015

[11]Proteomic Analysis of the Secretome of Porcine Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus. Li, Yuming,Wu, Zhuanchang,Liu, Ke,Qi, Pengfei,Xu, Jinpeng,Wei, Jianchao,Li, Beibei,Shao, Donghua,Shi, Yuanyuan,Qiu, Yafeng,Ma, Zhiyong.

[12]Proteomic analysis of castor bean tick Ixodes ricinus: a focus on chemosensory organs. Dani, Francesca Romana,Ban, Liping,Song, Limei,Pelosi, Paolo,Dani, Francesca Romana.

[13]Global iTRAQ-based proteomic profiling of Toxoplasma gondii oocysts during sporulation. Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Zhu, Xing-Quan,He, Shuai,Zhou, Dong-Hui,Elsheikha, Hany M.,He, Shuai,Li, Qian.

[14]Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus. Deng, Xiaoyu,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Yang, Guilian,Wang, Chunfeng,Ding, Chan,Yu, Shengqing,Liu, Xiufan,Wang, Chunfeng.

[15]PROTEOMICS ANALYSIS OF OVEREXPRESSED PLASMA PROTEINS IN RESPONSE TO COLD ACCLIMATION IN Ostrinia furnacalis. Shang, Qingli,Pan, Yiou,Peng, Tianfei,Yang, Shuang,Xi, Jinghui,Lu, Xin,Wang, Zhenying.

[16]Comparative proteomics analysis of pomegranate seeds on fruit maturation period (Punica granatum L.). Cao Shang-yin,Niu Juan,Li Hao-xian,Xue Hui,Chen Li-na,Zhang Fu-hong,Zhao Di-guang,Cao Da. 2015

[17]Hypothalamic protein profiles associated with inhibited feed intake of ducks fed with insufficient dietary arginine. Wang, C.,Xie, M.,Huang, W.,Xie, J. J.,Hou, S. S.,Wang, C.,Zheng, A. J..

[18]Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination. Ma, Qiaoli,Kang, Junmei,Zhang, Kun,Wang, Tenghua,Sun, Yan,Kang, Junmei,Long, Ruicai,Zhang, Tiejun,Yang, Qingchuan,Ma, Qiaoli,Xiong, Junbo.

[19]Comparative proteomic analyses reveal the changes of metabolic features in soybean (Glycine max) pistils upon pollination. Li, Ming,Yang, Pingfang,Li, Ming,Sha, Aihua,Zhou, Xinan.

[20]Proteomic Identification of Differentially Expressed Proteins in the Ligon lintless Mutant of Upland Cotton (Gossypium hirsutum L.). Zhao, Pi-Ming,Wang, Li-Li,Han, Li-Bo,Wang, Juan,Yao, Yuan,Wang, Hai-Yun,Luo, Yuan-Ming,Xia, Gui-Xian,Zhao, Pi-Ming,Wang, Li-Li,Han, Li-Bo,Wang, Juan,Yao, Yuan,Wang, Hai-Yun,Xia, Gui-Xian,Zhao, Pi-Ming,Wang, Li-Li,Han, Li-Bo,Wang, Juan,Yao, Yuan,Wang, Hai-Yun,Xia, Gui-Xian,Du, Xiong-Ming.

作者其他论文 更多>>