数字农科院2.0

Simultaneous production of algal biomass and lipid by heterotrophic cultivation of linoleic acid-rich oleaginous microalga Chlorella sorokiniana using high acetate dosage

文献类型： 外文期刊

作者： Guiping Gong;Linpei Liu;Bo Wu;Jianting Li;Mingxiong He;Guoquan Hu

作者机构：

关键词： Acetate bioconversion;Heterotrophic repeat fed-batch;Microalgal biomass;Microbial lipids;Trophic mode

期刊名称： Bioresource Technology

ISSN： 0960-8524

年卷期： 2024 年 399 卷

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： The low-cost carbon source, acetate, was utilized to feed a linoleic acid-rich Chlorella sorokiniana for microalgal biomass and lipid accumulation. Remarkably high tolerance capability to high acetate dosage up to 30 g/L was observed, with heterotrophy being the preferred trophic mode for algal growth and lipogenesis when supplemented 20 g/L acetate. Transcriptome analysis revealed a marked activation of pathways involved in acetate bioconversion and lipogenesis upon exposure to high-level of acetate. However, the enhancement of photorespiration inhibited photosynthesis, which ultimately led to a decrease in biomass and lipid under mixotrophy. Heterotrophic acetate-feeding generated more superior amino acid profiling of algal biomass and a predominant linoleic acid content (50 %). Heterotrophic repeat fed-batch strategy in 5 L fermenter significantly increased the growth performance and lipid titer, with the highest levels achieved being 23.4 g/L and 7.0 g/L, respectively. This work provides a viable approach for bio-products production through acetate-based heterotrophic algal cultivation.

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[1]Valorization of waste streams and C1 gases for sustainable food nutrients and value-added compounds production: Acetate as a promising intermediate. Guiping Gong,Bo Wu,Linpei Liu,Jianting Li,Qili Zhu,Mingxiong He,Guoquan Hu. 2023

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